Evaluation of phytochemical and <i>In vitro</i> anti-inflammatory activity of leaf and fruit extracts of <i>Casuarina equisetifolia</i>

Mamillapalli, Vani; Chapala, Ratna Harika; Sareddu, Tejaswi Komal Sai; Kondaveeti, Latha Sri; Pattipati, Santhi; Khantamneni, Padmalatha

doi:10.5958/2231-5713.2020.00025.2

Cited by 6 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the ethanolic extract demonstrated a significant dose-dependent anti-diarrheal activity by reducing the total number of faeces, weight, and volume of the intestinal contents ( Al-Snafi, 2016 ; Al-Snafi, 2018 ). Additionally, these species have demonstrated numerous biological activities, including antidiabetic, cardioprotective, antihyperlipidemic, nephroprotective, cytotoxic, hepatoprotective, anti-inflammatory, antioxidant, antimicrobial, and anti-influenza A virus (IAV) activities ( Zhang et al, 2010 ; Sriram, 2011 ; El-Tantawy and Saied, 2012 ; El-Tantawy et al, 2013b ; Salem et al, 2013 ; Hossen et al, 2014 ; Kantheti et al, 2014 ; Pawar and Nasreen, 2018 ; Mamillapalli et al, 2020 ; Muthuraj et al, 2020 ; Abd Al Haleem et al, 2022 ; Aboulthana et al, 2022 ; Al-Hadad and AL-Zubaidy, 2022 ; Xu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Casuarina glauca branchlets’ extract as a potential treatment for ulcerative colitis: chemical composition, in silico and in vivo studies

Mohamed,

El-Shafae,

Fikry

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Ulcerative colitis (UC) is an inflammatory bowel disease that is often resistant to current treatment options, leading to a need for alternative therapies. Herbal products have shown promise in managing various conditions, including UC. However, the potential of Casuarina glauca branchlets ethanolic extract (CGBRE) in treating UC has not been explored. This study aimed to analyze the chemical composition of CGBRE and evaluate its efficacy in UC treatment through in silico and in vivo experiments. LC-ESI-MS/MS was used to identify 86 compounds in CGBRE, with 21 potential bioactive compounds determined through pharmacokinetic analysis. Network pharmacology analysis revealed 171 potential UC targets for the bioactive compounds, including EGFR, LRRK2, and HSP90 as top targets, which were found to bind to key CGBRE compounds through molecular docking. Molecular docking findings suggested that CGBRE may be effective in the prevention or treatment of ulcerative colitis mediated by these proteins, where key CGBRE compounds exhibited good binding affinities through formation of numerous interactions. In vivo studies in rats with acetic acid-induced UC demonstrated that oral administration of 300 mg/kg CGBRE for 6 days reduced UC symptoms and colonic expression of EGFR, LRRK2, and HSP90. These findings supported the therapeutic potential of CGBRE in UC and suggested the need for further preclinical and clinical investigation.

show abstract

Section: Introductionmentioning

confidence: 99%

Casuarina glauca branchlets’ extract as a potential treatment for ulcerative colitis: chemical composition, in silico and in vivo studies

Mohamed,

El-Shafae,

Fikry

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

Krishnaveni

Samuel

2021

RJPT

View full text Add to dashboard Cite

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

show abstract

Phytochemical Analysis and Evaluation of the Antioxidant and Anti-Inflammatory activity of Canavalia gladiata

Rokkam,

Pinipay,

Kumar Paidi

et al. 2023

RJPT

View full text Add to dashboard Cite

The present study was done to determine whether scimitar bean or sword bean (Canavalia gladiata) seeds that had been stored for a year contained any proximate phytochemical equivalents and to assess their antioxidant and anti-inflammatory properties. In order of increasing polarity, hexane, ethyl acetate, and methanol were employed to extract phytochemicals from sword bean seeds. Hexane, ethyl acetate, and methanol extract each had a phytochemical yield of 0.23%, 0.26%, and 1.46%, respectively. Quantitative analysis of the extracts revealed that methanol extract had the highest levels of total polyphenolic contents (TPC) (17.74±1.929mg of gallic acid equivalents/g DM) and total tannin contents (TTC) (49.94±1.94mg of tannic acid equivalents/g DM), while hexane extract had the highest levels of total flavonoid contents (TFC) (9.06±1.197mg of quercetin equivalents/g DM). In C. gladiata seed extracts, tannins made up the majority of all phytochemicals. The solvent extract from C. gladiata seeds also showed strong antioxidant activity as measured by the total antioxidant and DPPH assays. Based on the inhibitory concentration (IC50) value of the DPPH assay, the ethyl acetate extract was shown to be the most effective antioxidant of all extracts (12.68±0.027µg/ml). Anti-inflammatory properties of extracts were evaluated by the egg albumin denaturation method, heat-induced and hypo-tonicityinduced HRBC membrane stabilization methods. The results of the heat-induced HRBC membrane stabilization method with an IC50of 613.39±0.975 µg/ml and hypo-tonicity induced HRBC membrane stabilization method with an IC50of 185.91±11.008 µg/ml revealed that ethyl acetate extract has significantly higher anti-inflammatory activity whereas methanol extract has shown higher anti-inflammatory activity as per egg albumin denaturation method with an IC50of 636±3.51µg/ml. Results showed that C. gladiata seed contains varying levels of phytochemical equivalents and indicate that the antioxidant and anti-inflammatory potential varied significantly and have a potential comparable to the standard drugs, ascorbic acid and Butylated Hydroxytoluene (BHT). Hence, C. gladiata seeds that have been stored for a year could be a good source of phytochemicalsand they can be used in pharmaceutical, cosmetic, and other formulations.

show abstract

Evaluation of phytochemical and In vitro anti-inflammatory activity of leaf and fruit extracts of Casuarina equisetifolia

Cited by 6 publications

References 0 publications

Casuarina glauca branchlets’ extract as a potential treatment for ulcerative colitis: chemical composition, in silico and in vivo studies

Casuarina glauca branchlets’ extract as a potential treatment for ulcerative colitis: chemical composition, in silico and in vivo studies

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

Phytochemical Analysis and Evaluation of the Antioxidant and Anti-Inflammatory activity of Canavalia gladiata

Contact Info

Product

Resources

About