Evaluation of physiological risk factors, oxidant-antioxidant imbalance, proteolytic and genetic variations of matrix metalloproteinase-9 in patients with pressure ulcer

Abstract

Pressure ulcer (PU) remains a common worldwide problem in all health care settings, it is synonymous with suffering. PU is a complex disease that is dependent on a number of interrelated factors. It involves multiple mechanisms such as physiological risk factors, chronic inflammation, oxidant–antioxidant imbalance and proteolytic attack on extracellular matrix by matrix metalloproteinases (MMP). Therefore, we propose that these wounds lead to molecular variations that can be detected by assessing biomarkers. In this study, we aimed to evaluate the major clinical elements and biological scars in Tunisian patients suffering from PU. Consistently, non-healing wound remains a challenging clinical problem. The complex challenges of the wound environment, involving nutrient deficiencies, bacterial infection, as well as the critical role played by inflammatory cells, should be considered because of their negative impact on wound healing. In addition, an imbalance between pro-oxidants and antioxidant systems seems to be more aggravated in patients with PU compared to healthy subjects. Of interest, this study provides further evidence to support a core role of the biological activity of MMP-9 in the pathogenesis of PU and indicates that the MMP9-1562 C/T (rs 3918242) functional polymorphism is associated with protection against this disease.

Curcumin accelerates cutaneous wound healing via multiple biological actions: The involvement of TNF‐α, MMP‐9, α‐SMA, and collagen

“…Collagen expression was increased by curcumin treatment in TNF‐α‐treated Hs68 cells in a dose‐dependent manner. A previous study reported that MMP‐9 alters the extracellular matrix (ECM) via the cleavage of matrix components as well as collagens and has detrimental effects on wound healing . Therefore, the levels of collagen secreted from TNF‐α‐treated Hs68 cells were determined in the presence of the indicated concentrations of curcumin (0‐2.5 μM) with or without an MMP‐9 inhibitor (CAS 1177749584) using the Sircol Collagen Assay (Figure D).…”

“…A previous study reported that MMP-9 alters the extracellular matrix (ECM) via the cleavage of matrix components as well as collagens and has detrimental effects on wound healing. 24 Therefore, the levels of collagen secreted from TNF-α-treated Hs68 cells were determined in the presence of the indicated concentrations of curcumin (0-2.5 μM) with or without an MMP-9 inhibitor (CAS 1177749584) using the Sircol Collagen Assay ( Figure 5D). The levels of collagen were significantly increased by curcumin treatment in TNF-α-treated fibroblasts.…”

Impaired wound healing: facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine

“…Different FXIII polymorphisms have also been shown to modulate and even have protective effects against ulcers progression [25]. Similarly, the SNP-1562C/T detected in the MMP-9 gene, which downregulates MMP-9 expression, has been shown to have a protective effect against pressure ulcers [28].…”

A functional polymorphism at miR‑491‑5p binding site in the 3'UTR of MMP9 gene confers increased risk for pressure ulcers after hip fracture

“…This evidence indicated that this protease could destruct the extracellular proteins, receptors and growth factors critical for PU healing (7). It has been shown that the activity of serum pro-MMP9 was elevated more in those with PU than the healthy people (34).…”