Evaluation of Physicochemical Activity of Anticancer Fusion Proteins; Enterocin A- R type pyocin-Lactocin-Ligand Against Gastric Cancer Cell Line by Real-Time RT PCR Technique

Jalalvand, Neda; Esmaeili, Davoud; Bashi, Moha mmad Mehdi Moghani; Raiszadeh, Mohammad; Naeimi, Sirous

doi:10.1007/s10989-021-10158-3

Cited by 9 publications

(4 citation statements)

References 14 publications

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“…When this recombinant protein was exposed to AGS cell line, it resulted in a signi cant increase in the expression of Bax and caspase 3 genes and a signi cant decrease in Bcl2 gene expression compared to the GAPDH reference gene. 27 In our study, the anticancer effect of peptide fusion, bacteriocins and speci c ligand of AGS cell line were investigated. In general, the recombinant protein induced apoptosis in the AGS cell line, and it can be suggest that this recombinant protein activated apoptosis internally, leading to cell death.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of recombinant protein was then evaluated using polyclonal antibodies. 27 Cell treatment and study of apoptosis each cavity of 96-well plate, 100,000 AGS cells from the cell bank of the Genetic and Biological Resources Center of Iran (IBRC C10071) were poured and incubated for 24 hours in a CO2-containing incubator at 37°C to allow the cells to adhere to the bottom of the plate. Then, the proliferation and adhesion of cells to the bottom of the cavities were examined, and cases where the cell size was much lower or much higher were removed.…”

Section: Methodsmentioning

confidence: 99%

“…Initially, a recombinant gene cassette containing anticancer sequences, lactocin-enterocin, pyocin and speci c ligand genes was designed and their sequences were prepared for synthesis and cloning in pET22b expression vector from Biomatik Corporation. 27 Transformation of the generated vector into susceptible cells was performed by heat shock method.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Apoptotic Activity of Protein Fusion Enterocin A- Pyocin R-Lactocin, Bacteriocins and Specific Ligand of Gastric Cancer Cell Line (AGS)

Jalalvand

Bashi

Raiszadeh

et al. 2021

Preprint

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Conventional antimicrobial and anti-cancer treatments, including chemotherapy, surgery, radiation therapy, etc., have all been associated with antimicrobial resistance (AMR) and side effects on healthy cell damage. Recently, bacteriocins have emerged as a promising option in antimicrobial and anticancer therapies.In this study, EntA-PynR-Lac gene sequence was obtained using bioinformatics software. The designed fusion gene was synthesized and cloned into the pET22b expression vector and transferred to the engineered E. coli BL21 bacterium for expression of the recombinant protein. The three-dimensional structures and stability of the designed recombinant protein were evaluated. Confirmation and purification of the recombinant protein was performed by Western blotting and nickel column chromatography and determination of cell lethality of different concentrations of the recombinant protein against AGS cell line by MTT technique was conducted. Treatment of cells with different concentrations of protein to evaluate the apoptosis of AGS cells was performed by flow cytometry. The results showed that the percentage of cells treated with recombinant protein at a concentration of 80µg/ml with total apoptosis was 46.91% and increased by 36.25% compared to untreated cells. Due to the anti-apoptotic properties of fusion protein, it can be used to inhibit cancer cells as a therapeutic supplement and prevention.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Evaluation of Apoptotic Activity of Protein Fusion Enterocin A- Pyocin R-Lactocin, Bacteriocins and Specific Ligand of Gastric Cancer Cell Line (AGS)

Jalalvand

Bashi

Raiszadeh

et al. 2021

Preprint

Self Cite

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“…Furthermore, some bacteriocins exhibited antifungal and/or antiviral activity with an inhibitor potential of sporulating bacteria such as Clostridium botulinum and Bacillus cereus as well as an anti-endospores potency [261]. Outstandingly, these enterocins also displayed anti-cancer activities [262]. These attributes provide the justification to nominate bacteriocinogenic Enterococcus strains as relevant candidates for food, feed, human and animal health applications.…”

Section: Concluding Remarks and Future Outlookmentioning

confidence: 99%

Enterococcus spp.: Is It a Bad Choice for a Good Use—A Conundrum to Solve?

et al. 2021

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Since antiquity, the ubiquitous lactic acid bacteria (LAB) Enterococci, which are just as predominant in both human and animal intestinal commensal flora, have been used (and still are) as probiotics in food and feed production. Their qualities encounter several hurdles, particularly in terms of the array of virulence determinants, reflecting a notorious reputation that nearly prevents their use as probiotics. Additionally, representatives of the Enterococcus spp. genus showed intrinsic resistance to several antimicrobial agents, and flexibility to acquire resistance determinants encoded on a broad array of conjugative plasmids, transposons, and bacteriophages. The presence of such pathogenic aspects among some species represents a critical barrier compromising their use as probiotics in food. Thus, the genus neither has Generally Recognized as Safe (GRAS) status nor has it been included in the Qualified Presumption of Safety (QPS) list implying drastic legislation towards these microorganisms. To date, the knowledge of the virulence factors and the genetic structure of foodborne enterococcal strains is rather limited. Although enterococcal infections originating from food have never been reported, the consumption of food carrying virulence enterococci seems to be a risky path of transfer, and hence, it renders them poor choices as probiotics. Auspiciously, enterococcal virulence factors seem to be strain specific suggesting that clinical isolates carry much more determinants that food isolates. The latter remain widely susceptible to clinically relevant antibiotics and subsequently, have a lower potential for pathogenicity. In terms of the ideal enterococcal candidate, selected strains deemed for use in foods should not possess any virulence genes and should be susceptible to clinically relevant antibiotics. Overall, implementation of an appropriate risk/benefit analysis, in addition to the case-by-case assessment, the establishment of a strain’s innocuity, and consideration for relevant guidelines, legislation, and regulatory aspects surrounding functional food development seem to be the crucial elements for industries, health-staff and consumers to accept enterococci, like other LAB, as important candidates for useful and beneficial applications in food industry and food biotechnology. The present review aims at shedding light on the world of hurdles and limitations that hampers the Enterococcus spp. genus and its representatives from being used or proposed for use as probiotics. The future of enterococci use as probiotics and legislation in this field are also discussed.

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Evaluation and Efficacy Modified Carvacrol and Anti-cancer Peptide Against Cell Line Gastric AGS

Elahi

Hekmati

Esmaeili

et al. 2022

Int J Pept Res Ther

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Evaluation of Physicochemical Activity of Anticancer Fusion Proteins; Enterocin A- R type pyocin-Lactocin-Ligand Against Gastric Cancer Cell Line by Real-Time RT PCR Technique

Cited by 9 publications

References 14 publications

Evaluation of Apoptotic Activity of Protein Fusion Enterocin A- Pyocin R-Lactocin, Bacteriocins and Specific Ligand of Gastric Cancer Cell Line (AGS)

Evaluation of Apoptotic Activity of Protein Fusion Enterocin A- Pyocin R-Lactocin, Bacteriocins and Specific Ligand of Gastric Cancer Cell Line (AGS)

Enterococcus spp.: Is It a Bad Choice for a Good Use—A Conundrum to Solve?

Evaluation and Efficacy Modified Carvacrol and Anti-cancer Peptide Against Cell Line Gastric AGS

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