Evaluation of phylogenetic relationships among fission yeast by nDNA/nDNA reassociation and conventional taxonomic criteria

Martini, Ann Vaughan

doi:10.1002/yea.320070108

Cited by 25 publications

(11 citation statements)

References 26 publications

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“…malidevorans (Rankine and Fornachon 1964 ). Before its merging under the species S. pombe (Vaughan Martini 1991 ; Vaughan-Martini and Martini 1998 ), the defining taxonomic feature of S. malidevorans is its inability to utilize maltose (Yarrow 1984 ; Kocková-Kratochvílová et al 1985 ; Yamada et al 1987 ). Thus, we reasoned that if there are Mal - S. pombe strains different from CBS5557, they might have also been deposited in microbial strain collections under the species name S. malidevorans .…”

Section: Resultsmentioning

confidence: 99%

Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast

Suo

2015

Genome Biol Evol

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Although the fission yeast Schizosaccharomyces pombe is a well-established model organism, studies of natural trait variations in this species remain limited. To assess the feasibility of segregant-pool-based mapping of phenotype-causing genes in natural strains of fission yeast, we investigated the cause of a maltose utilization defect (Mal-) of the S. pombe strain CBS5557 (originally known as Schizosaccharomyces malidevorans). Analyzing the genome sequence of CBS5557 revealed 955 nonconservative missense substitutions, and 61 potential loss-of-function variants including 47 frameshift indels, 13 early stop codons, and 1 splice site mutation. As a side benefit, our analysis confirmed 146 sequence errors in the reference genome and improved annotations of 27 genes. We applied bulk segregant analysis to map the causal locus of the Mal- phenotype. Through sequencing the segregant pools derived from a cross between CBS5557 and the laboratory strain, we located the locus to within a 2.23-Mb chromosome I inversion found in most S. pombe isolates including CBS5557. To map genes within the inversion region that occupies 18% of the genome, we created a laboratory strain containing the same inversion. Analyzing segregants from a cross between CBS5557 and the inversion-containing laboratory strain narrowed down the locus to a 200-kb interval and led us to identify agl1, which suffers a 5-bp deletion in CBS5557, as the causal gene. Interestingly, loss of agl1 through a 34-kb deletion underlies the Mal- phenotype of another S. pombe strain CGMCC2.1628. This work adapts and validates the bulk segregant analysis method for uncovering trait-gene relationship in natural fission yeast strains.

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Section: Resultsmentioning

confidence: 99%

Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast

Suo

2015

Genome Biol Evol

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“…Since the initial discovery of S. pombe (Linder, 1893), several other species have been described in this genus including Schizosaccharomyces liquefaciens (Osterwalder, 1924) and Schizosaccharomyces slooffiae (Kumbhojkar, 1972), but a closer examination of nuclear DNA/nuclear DNA reassociation kinetics (Martini, 1991) revealed them to be conspecific with one of the three aforementioned species. Here, we describe a new species of fission yeast on the basis of phenotypic differences, genome sequencing and phylogenetic analyses.…”

Section: Introductionmentioning

confidence: 99%

Schizosaccharomyces cryophilus sp. nov., a new species of fission yeast

Helston¹,

Box²,

Tang³

et al. 2010

FEMS Yeast Research

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The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the other fission yeast species. Although morphologically similar to S. octosporus, analysis of several rapidly evolving sequences, including the D1/D2 divergent domain of the large subunit (LSU) rRNA gene, the RNA subunit of RNAse P and the internal transcribed spacer elements, revealed significant divergence from any previously characterized Schizosaccharomyces strain. Based on phylogenetic analysis of the D1/D2 domain of the LSU rRNA gene, S. octosporus is the closest known relative of S. cryophilus, with the sequences of the two species differing by 25 nucleotide substitutions (>4%). Sequencing of the S. cryophilus genome and phylogenetic analysis of all 1 : 1 protein orthologs confirmed this observation, and together with morphological and physiological characterization, supports the assignment of S. cryophilus as a new species within the genus Schizosaccharomyces. The type strain of the new species is NRRL Y-48691(T) (=NBRC 106824(T)=CBS 11777(T)).

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“…However, immediately after cell division, new cells formed have a more rounded shape due to the turgor pressure [2]. It has a peculiar mode of vegetative reproduction by fission (cross-wall formation) instead of budding, which is more common among yeasts [3]. Cells are separated by the formation of a transverse septum.…”

Section: Origin and Features Of Schizosaccharomyces Pombementioning

confidence: 99%

Schizosaccharomyces pombe: A Promising Biotechnology for Modulating Wine Composition

et al. 2018

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There are numerous yeast species related to wine making, particularly non-Saccharomyces, that deserve special attention due to the great potential they have when it comes to making certain changes in the composition of the wine. Among them, Schizosaccharomyces pombe stands out for its particular metabolism that gives it certain abilities such as regulating the acidity of wine through maloalcoholic fermentation. In addition, this species is characterized by favouring the formation of stable pigments in wine and releasing large quantities of polysaccharides during ageing on lees. Moreover, its urease activity and its competition for malic acid with lactic acid bacteria make it a safety tool by limiting the formation of ethyl carbamate and biogenic amines in wine. However, it also has certain disadvantages such as its low fermentation speed or the development of undesirable flavours and aromas. In this chapter, the main oenological uses of Schizosaccharomyces pombe that have been proposed in recent years will be reviewed and discussed.

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Evaluation of phylogenetic relationships among fission yeast by nDNA/nDNA reassociation and conventional taxonomic criteria

Cited by 25 publications

References 26 publications

Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast

Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast

Schizosaccharomyces cryophilus sp. nov., a new species of fission yeast

Schizosaccharomyces pombe: A Promising Biotechnology for Modulating Wine Composition

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