Evaluation of phenotypic diversity of the endangered orchid (Orchis mascula): Emphasizing on breeding, conservation and development

Ebrahimi, Asa; Asadi, AliAkbar; Monfared, Sajad Rashidi; Sahebi, Mohammad; Rezaee, Sadra; Khaledian, Yasin

doi:10.1016/j.sajb.2020.05.013

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…These traits mentioned above also had the highest variation percentage under the effects of land cover and population (Table 1). A phenotypic study on O. mascula populations by 26 also indicated high variation for tuber characteristics, BFW & BDW in particular. Additionally, marker-assisted phenotypic trait selection may provide heritable traits with high economic value.…”

Section: Discussionmentioning

confidence: 93%

“…The results of the current study were, to a large extent, consistent with these studies. In the comprehensive phenotypic study of Ebrahimi, et al 26 . quantitative floral traits showed considerably higher variation than the results reported here; however, the number of flowers had the highest coefficient of variation (41.34%), similar to our results (62.07%, Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Besides the ongoing over-exploitation of the wild population of tuberous orchids, climate change may also hasten the extinction of orchid species 24,25 . Agricultural expansion, urbanization, and silviculture have exacerbated the loss of genetic diversity in orchid populations 26,27 . Such problematic circumstances have already taken their toll on orchids in Iran, and some orchid species, including Orchis mascula L., have been classified as endangered 26 .…”

mentioning

confidence: 99%

“…Agricultural expansion, urbanization, and silviculture have exacerbated the loss of genetic diversity in orchid populations 26,27 . Such problematic circumstances have already taken their toll on orchids in Iran, and some orchid species, including Orchis mascula L., have been classified as endangered 26 . Therefore, conservation strategies are needed to maintain the genetic diversity in the remaining populations and assure the species' long-term viability 28,29 .…”

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confidence: 99%

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The impact of environmental and climatic variables on genetic diversity and plant functional traits of the endangered tuberous orchid (Orchis mascula L.)

Mafakheri

Bakhshipour

Omrani

et al. 2022

Sci Rep

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Understanding how environmental factors shape patterns of genetic and phenotypic variations in a species is necessary for conservation and plant breeding. However, these factors have not yet been completely understood in tuberous orchid species used to make ‘Salep’, an important ingredient in traditional medicine and beverages in middle eastern countries and India. In many areas, increasing demand has pushed species to the brink of extinction. In this study, 198 genotypes from 18 populations of the endangered species Orchis mascula L. spanning a large-scale climatic gradient in northern Iran were used to investigate patterns of genetic diversity and plant functional traits. Populations were sampled from three land cover types (woodland, shrubland, and pastureland/grassland). Plant height, stem length, number of flowers, bulb fresh and dry weight, glucomannan, and starch concentrations showed high variation among populations and were significantly related to land cover type. In general, genetic diversity was high, particularly in those from eastern Hyrcanian; additionally, populations showed a high level of genetic differentiation (G'st = 0.35) with low gene flow (Nm = 0.46). The majority of genetic differentiation occurred within populations (49%) and land cover types (20%). The population structural analysis using the AFLP marker data in K = 4 showed a high geographical affinity for 198 O. mascula genotypes, with some genotypes having mixed ancestry. Temperature and precipitation were found to shape genetic and phenotypic variation profoundly. Significant isolation by the environment was observed, confirming the strong effect of environmental variables on phenotypic and genetic variation. Marker-trait association studies based on MLM1 and MLM2 models revealed significant associations of P-TGG + M-CTT-33 and E-AGG + M-CGT-22 markers with plant height and glucomannan content. Overall, a combination of large-scale climatic variables and land cover types significantly shaped genetic diversity and functional trait variation in O. mascula populations.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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The impact of environmental and climatic variables on genetic diversity and plant functional traits of the endangered tuberous orchid (Orchis mascula L.)

Mafakheri

Bakhshipour

Omrani

et al. 2022

Sci Rep

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“…Consequently, combining phenotypic and SSR marker analysis to a certain extent can effectively reveal the genetic variation of A. cremastogyne . In comparison to other tree species, A. cremastogyne has a lower phenotypic differentiation coefficient than P. bournei (70.83%) [ 49 ], while this is higher than Orchis mascula (20.00%) [ 50 ]. It is also similar to Juglans mandshurica (50.31%) [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Variation Analysis and Excellent Clone Selection of Alnus cremastogyne from Different Provenances

Zheng,

Feng,

et al. 2023

Plants

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Alnus cremastogyne is a rapidly growing broad-leaved tree species that is widely distributed in southwest China. It has a significant economic and ecological value. However, with the expansion of the planting area, the influence of phenotypic variation and differentiation on Alnus cremastogyne has increased, resulting in a continuous decline in its genetic quality. Therefore, it is crucial to investigate the phenotypic variation of Alnus cremastogyne and select excellent breeding materials for genetic improvement. Herein, four growth-related phenotypic traits (diameter at breast height, the height of trees, volume, height under the branches) and twelve reproductive-related phenotypic traits (fresh weight of single cone, dry weight of single cone, seed weight per plant, thousand kernel weight, cone length, cone width, cone length × cone width, fruit shape index, seed rate, germination rate, germination potential, germination index) of 40 clones from four provenances were measured and analyzed. The phenotypic variation was comprehensively evaluated by correlation analysis, principal component analysis and cluster analysis, and excellent clones were selected as breeding materials. The results revealed that there were abundant phenotypic traits variations among and within provenances. Most of the phenotypic traits were highly significant differences (p < 0.01) among provenances. The phenotypic variation among provenances (26.36%) was greater than that of within provenances clones (24.80%). The average phenotypic differentiation coefficient was accounted for 52.61% among provenances, indicating that the phenotypic variation mainly came from among provenances. The coefficient of variation ranged from 9.41% (fruit shape index) to 97.19% (seed weight per plant), and the repeatability ranged from 0.36 (volume) to 0.77 (cone width). Correlation analysis revealed a significantly positive correlation among most phenotypic traits. In principal component analysis, the cumulative contribution rate of the first three principal components was 79.18%, representing the main information on the measured phenotypic traits. The cluster analysis revealed four groups for the 40 clones. Group I and group II exhibited better performance phenotypic traits as compared with group III and group IV. In addition, the four groups are not clearly clustered following the distance from the provenance. Employing the multi-trait comprehensive evaluation method, 12 excellent clones were selected, and the average genetic gain for each phenotypic trait ranged from 4.78% (diameter at breast height) to 32.05% (dry weight of single cone). These selected excellent clones can serve as candidate materials for the improvement and transformation of Alnus cremastogyne seed orchards. In addition, this study can also provide a theoretical foundation for the genetic improvement, breeding, and clone selection of Alnus cremastogyne.

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Population and Individual Multivariate Analysis of Barberry Genotypes: Implications for Breeding, Development, and Food Security

et al. 2022

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Evaluation of phenotypic diversity of the endangered orchid (Orchis mascula): Emphasizing on breeding, conservation and development

Cited by 6 publications

References 23 publications

The impact of environmental and climatic variables on genetic diversity and plant functional traits of the endangered tuberous orchid (Orchis mascula L.)

The impact of environmental and climatic variables on genetic diversity and plant functional traits of the endangered tuberous orchid (Orchis mascula L.)

Phenotypic Variation Analysis and Excellent Clone Selection of Alnus cremastogyne from Different Provenances

Population and Individual Multivariate Analysis of Barberry Genotypes: Implications for Breeding, Development, and Food Security

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