Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1

Alharbi, Mona G.; Al-Hindi, Rashad R.; Alotibi, Ibrahim; Azhari, Sheren; Farsi, Reem M.

doi:10.1016/j.heliyon.2023.e13077

Cited by 2 publications

(1 citation statement)

References 68 publications

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“…Phage L223 exhibited a short latent period (30 min) and a large burst size of L223 (515 PFU/cell) in one step growth curve. This latent time was similar to phage phiPT1, STGO-35-1, AUFM_Sc3 but shorter than a cocktail of four phages including vB_SnwM_CGG4-1, vB_SnwM_CGG4-2, vB_SnwM_CGG3-1 and vB_SnwM_CGG3-2 (53 to 65 min), P22 (45 min) and st104a (60 min) 38 – 42 . Moreover, the burst size of L223 was comparable to BPSELC-1 (500 PFU/cell) but larger than vB_SalP_TR2 (211 PFU/cell), OSY-STA (176 pfu/cell), OSY-SHC (256 PFU/cell), SHWT1 (150 PFU/cell) and others 43 – 45 .…”

Section: Discussionmentioning

confidence: 66%

Phenotypic characterization and genomic analysis of a Salmonella phage L223 for biocontrol of Salmonella spp. in poultry

Khan,

Islam,

Barua

et al. 2024

Sci Rep

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The escalating incidence of foodborne salmonellosis poses a significant global threat to food safety and public health. As antibiotic resistance in Salmonella continues to rise, there is growing interest in bacteriophages as potential alternatives. In this study, we isolated, characterized, and evaluated the biocontrol efficacy of lytic phage L223 in chicken meat. Phage L223 demonstrated robust stability across a broad range of temperatures (20–70 °C) and pH levels (2–11) and exhibited a restricted host range targeting Salmonella spp., notably Salmonella Typhimurium and Salmonella Enteritidis. Characterization of L223 revealed a short latent period of 30 min and a substantial burst size of 515 PFU/cell. Genomic analysis classified L223 within the Caudoviricetes class, Guernseyvirinae subfamily and Jerseyvirus genus, with a dsDNA genome size of 44,321 bp and 47.9% GC content, featuring 72 coding sequences devoid of antimicrobial resistance, virulence factors, toxins, and tRNA genes. Application of L223 significantly (p < 0.005) reduced Salmonella Typhimurium ATCC 14,028 counts by 1.24, 2.17, and 1.55 log CFU/piece after 2, 4, and 6 h of incubation, respectively, in experimentally contaminated chicken breast samples. These findings highlight the potential of Salmonella phage L223 as a promising biocontrol agent for mitigating Salmonella contamination in food products, emphasizing its relevance for enhancing food safety protocols.

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