Evaluation of Permeate Flux Rate and Membrane Fouling in Dead-End Microfiltration of Primary Sewage Effluent

Modise, C. M.; Shan, Hongwei; Neufeld, Ronald D.; Vidic, Radisav D.

doi:10.1089/ees.2005.22.427

Cited by 6 publications

(4 citation statements)

References 16 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The membrane resistance can also be estimated using the Kozeny‐Carman equation (Eq. 2) under the assumption that each membrane consists of closely packed spheres 24 …”

Section: Resultsmentioning

confidence: 99%

Evaluation of cell disruption effects on primary recovery of antibody fragments using microscale bioprocessing techniques

Rayat

Micheletti

Lye

2010

Biotechnology Progress

View full text Add to dashboard Cite

Intracellular antibody Fab' fragments periplasmically expressed in Escherichia coli require the release of Fab' from the cells before initial product recovery. This work demonstrates the utility of microscale bioprocessing techniques to evaluate the influence of different cell disruption operations on subsequent solid-liquid separation and product recovery. Initially, the industrial method of Fab' release by thermochemical extraction was established experimentally at the microwell scale and was observed to yield Fab' release consistent with the larger scale process. The influence of two further cell disruption operations, homogenization and sonication, on subsequent Fab' recovery by microfiltration was also examined. The results showed that the heat-extracted cells give better dead-end microfiltration performance in terms of permeate flux and specific cake resistance. In contrast, the cell suspensions prepared by homogenization and sonication showed more efficient product release but with lower product purity and poorer microfiltration performance. Having established the various microscale methods the linked sequence was automated on the deck of a laboratory robotic platform and used to show how different conditions during thermochemical extraction impacted on the optimal performance of the linked unit operations. The results illustrate the power of microscale techniques to evaluate crucial unit operation interactions in a bioprocess sequence using only microliter volumes of feed.

show abstract

“…The membrane resistance can also be estimated using the Kozeny‐Carman equation (Eq. 2) under the assumption that each membrane consists of closely packed spheres 24 …”

Section: Resultsmentioning

confidence: 99%

Evaluation of cell disruption effects on primary recovery of antibody fragments using microscale bioprocessing techniques

Rayat

Micheletti

Lye

2010

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…FASP would be considered a form of dead-end filtration in which the feedstock is perpendicular to the membrane . In such a strategy, membrane fouling can be an issue, giving rise to reduced analyte recovery. , Electrofiltration can circumvent this by applying an electric field to oppose the motion of charged particles away from the filter . Cross-flow filtration is another strategy for sample purification wherein the fluid flows tangential to the dialysis membrane .…”

Section: Introductionmentioning

confidence: 99%

Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins though Transmembrane Electrophoresis

et al. 2016

View full text Add to dashboard Cite

Membrane proteins are underrepresented in proteome analysis platforms because of their hydrophobic character, contributing to decreased solubility. Sodium dodecyl sulfate is a favored denaturant in proteomic workflows, facilitating cell lysis and protein dissolution; however, SDS impedes MS detection and therefore must be removed prior to analysis. Although strategies exist for SDS removal, they provide low recovery, purity, or reproducibility. Here we present a simple automated device, termed transmembrane electrophoresis (TME), incorporating the principles of membrane filtration, but with an applied electric current to ensure near-complete (99.9%) removal of the surfactant, including protein-bound SDS. Intact proteins are recovered in solution phase in high yield (90-100%) within 1 h of operation. The strategy is applied to protein standards and proteome mixtures, including an enriched membrane fraction from E. coli, resulting in quality MS spectra free of SDS adducts. The TME platform is applicable to both bottom-up MS/MS as well as LC-ESI-MS analysis of intact proteins. SDS-depleted fractions reveal a similar number of protein identifications (285) compared wit a non-SDS control (280), being highly correlated in terms of protein spectral counts. This fully automated approach to SDS removal presents a viable tool for proteome sample processing ahead of MS analysis. Data are available via ProteomeXchange, identifier PXD003941.

show abstract

“…This study is an extension of previous research on the application of microfiltration in treating combined sewer overflows (CSOs) (Bendick et al, 2004;Modise et al, 2005), which demonstrated that a 0.2-lm membrane would be the most appropriate membrane for the treatment of CSO wastewater. This study focuses on the fouling pattern of microfiltration of primary and secondary effluents from municipal wastewater treatment plants on polymeric microfiltration membranes.…”

Section: Introductionmentioning

confidence: 85%

Irreversible Fouling During Multicycle Microfiltration of Wastewater Effluent

Shan¹,

Neufeld²

2007

Water Environment Research

View full text Add to dashboard Cite

This study focused on irreversible fouling during microfiltration of primary and secondary effluents from municipal wastewater treatment plants. Flow resistances were calculated from the sum of clean membrane resistances, resultant cake layer resistances, and consequent irreversible fouling resistances. Results from a dead‐end cell experimental system showed that the accumulated cake resistance was dominating for microfiltration of primary/secondary effluents. Suspended solids in the primary and secondary effluents had a similar compressibility index, n, with a value of approximately 0.5, indicating that they were moderately compressible particles. The value of irreversible resistance is dependent on the intensity of membrane cleaning; however, for a given membrane cleaning strategy, this value steadily increased and reached a maximum after approximately 6 cycles of filtration and cleaning. This study provided an explanation for the significant drop of throughput flux in the early application of membrane processes, and a plateau flux approached correspondingly.

show abstract

Evaluation of Permeate Flux Rate and Membrane Fouling in Dead-End Microfiltration of Primary Sewage Effluent

Cited by 6 publications

References 16 publications

Evaluation of cell disruption effects on primary recovery of antibody fragments using microscale bioprocessing techniques

Evaluation of cell disruption effects on primary recovery of antibody fragments using microscale bioprocessing techniques

Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins though Transmembrane Electrophoresis

Irreversible Fouling During Multicycle Microfiltration of Wastewater Effluent

Contact Info

Product

Resources

About