Evaluation of Periodontal Ligament Cell Viability in Honey as a Storage Media at Different Time Intervals: An In Vitro Study

Storage Media to Preserve Avulsioned Teeth Maintaining the Viability of Periodontal Cells: A Review

“…(1)(2)(3)(4) This trauma is very common among children aged 7-9 years old, and the maxillary central incisors are the most commonly affected teeth; additionally, dental avulsion causes functional, psychological and aesthetic problems. (5)(6)(7)(8) The ideal management of dental avulsion consists of immediate reimplantation within the socket in a time interval of 5 minutes, which improves the repair of the periodontal ligament (LPD) and significantly reduces root resorption and ankylosis. (2)(3)(4)(5)(6)9) The success of reimplantation of a dental organ depends largely on the presence of viable cells (fibroblasts) on the surface of the root, and an appropriate storage medium ensures the viability of these cells, thus improving the prognosis of the affected piece.…”

“…(5)(6)(7)(8) The ideal management of dental avulsion consists of immediate reimplantation within the socket in a time interval of 5 minutes, which improves the repair of the periodontal ligament (LPD) and significantly reduces root resorption and ankylosis. (2)(3)(4)(5)(6)9) The success of reimplantation of a dental organ depends largely on the presence of viable cells (fibroblasts) on the surface of the root, and an appropriate storage medium ensures the viability of these cells, thus improving the prognosis of the affected piece. (6.10) There is little knowledge regarding how to treat dental avulsions.…”

“…(1,9) It has been shown that the capacity of the storage medium to maintain the viability of the LPD cells is more important than the duration of time that the piece is outside the alveolus. (2,6,11) The use of an inadequate transport medium can cause necrosis of the periodontal ligament, root resorption and tooth loss. (1,9,12) It has been reported that the periodontal ligament has a constant blood supply, a pH of 7.2 and an osmolarity of 280 to 300 mOsm/L.…”

“…An ideal storage and transport medium must meet the following characteristics: neutral pH between 7.2 and 7.4, osmolality between 230 and 400 mOsm/kg, temperature of 9.5 °C, sterile, contain nutrients, available at the time of injury, long useful life and low cost. (1)(2)(3)(4)(5)(6)(7)(11)(12)(13)(14) In the search for an ideal storage medium, these characteristics of a wide variety of products have been studied as possible media of transport for avulsed teeth that are divided into three groups: milk and its derivatives; natural products; and rehydration solutions. (5)(6)(7)(8)15,16) The purpose of this research is to identify the most effective media for the storage and transport of avulsed permanent teeth, according to the viability of the periodontal ligament cells.…”

“…(1)(2)(3)(4)(5)(6)(7)(11)(12)(13)(14) In the search for an ideal storage medium, these characteristics of a wide variety of products have been studied as possible media of transport for avulsed teeth that are divided into three groups: milk and its derivatives; natural products; and rehydration solutions. (5)(6)(7)(8)15,16) The purpose of this research is to identify the most effective media for the storage and transport of avulsed permanent teeth, according to the viability of the periodontal ligament cells.…”