Evaluation of periodontal ligament cell viability in different storage media based on human <scp>PDL</scp> cell culture experiments—A systematic review

Osmanovic, Ahmed; Halilovic, Sabina; Kurtovic-Kozaric, Amina; Hadziabdic, Naida

doi:10.1111/edt.12437

Cited by 40 publications

(58 citation statements)

References 26 publications

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“…To date, various tooth‐storage media have been tested in order to find one that can preserve the vitality and functions of PDLFs, promote PDL regeneration, and prevent post‐replantation complications. So far, HBSS and milk are still the tooth‐preserving solutions recommended as a result of their characteristics and ability to maintain the viability of PDLFs . Nevertheless, each has some disadvantages: HBSS is not easily accessible at the scene of accidents; and milk has been shown to have some negative effects on cells and on the formation of PDL tissue .…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, the efficacy of various types of storage media in maintaining PDLF viability in vitro was mainly determined either by testing the storage solution on cultured PDL cells or by direct immersion of the extracted tooth in the test media followed by quantification of viable cells . Discrepancies between different studies in the performance of various types of storage media may arise from the storage method chosen and other experimental factors, including duration of exposure to the medium, temperature, the source of storage media, and the assessment methods chosen . In our study, we investigated the ability of almond milk and cow's milk to maintain the viability and functions of cultured PDLFs following simulated tooth avulsion in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Cow's milk is recommended for storage of avulsed teeth owing to its neutral pH, physiological osmolality, abundant nutrients, and growth factors. Besides, it has gained wide acceptance because it is inexpensive and, most importantly, easily accessible at the scene of an accident . In addition, long‐shelf‐life Ultra‐High Temperature (UHT) processed milk has been shown to have comparable efficiency to pasteurized milk in maintaining PDL cell viability .…”

mentioning

confidence: 99%

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Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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Milk remains one of the most frequently recommended solutions for storage of avulsed teeth because it can maintain cell viability and is easily accessible. However, some negative effects of milk on avulsed teeth have been reported, just as the effects of milk on the long‐term functions of cells are not clear. This study aimed to evaluate the effects of different types of milk on the viability, proliferation, and functions of periodontal ligament fibroblasts (PDLF)s in vitro. Human PDLFs were culture‐medium depleted for 5 min and stored in Hanks’ balanced salt solution (HBSS), whole cow's milk, low‐fat cow's milk, or almond milk for 1 h at 25°C. Cell viability and proliferation were assessed using MTT assays. Expression of the genes encoding type I collagen and its modifying enzymes were analyzed using real‐time PCR. Collagen matrix production was evaluated using Picrosirius red polarization. Our results showed the overall efficiency of low‐fat cow's milk in maintaining the viability and proliferation of PDLFs, and in enhancing the process of collagen production. Almond milk storage resulted in the highest rate of PDLF proliferation, and comparable collagen biosynthesis ability to the control. Therefore, besides low‐fat cow's milk, almond milk may potentially be an alternative tooth‐storage medium for PDLF preservation and PDL tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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“…Most complications following traumatic tooth avulsion and subsequent replantation are related to compromised healing of the pulp and periodontal ligament (PDL) . If the initial trauma caused minor damage to the PDL and cementum, a mild osteoclastic activity on the root surface will occur.…”

Section: Introductionmentioning

confidence: 99%

“…Most complications following traumatic tooth avulsion and subsequent replantation are related to compromised healing of the pulp and periodontal ligament (PDL). [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] If the initial trauma caused minor damage to the PDL and cementum, a mild osteoclastic activity on the root surface will occur. This process has been termed repair-related or surface resorption 19 and is transient, not progressive.…”

Section: Introductionmentioning

confidence: 99%

Risk of ankylosis of 400 avulsed and replanted human teeth in relation to length of dry storage: A re‐evaluation of a long‐term clinical study

Lauridsen

Andreasen

Bouaziz

et al. 2019

Dental Traumatology

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Background/Aims Ankylosis and subsequent tooth loss is a serious complication following tooth avulsion and replantation. The aim of the study was to evaluate the risk of ankylosis of human teeth replanted after various periods of dry storage prior to replantation. The material originates from a previously reported clinical study of 400 replanted teeth. Methods Initial treatment, clinical and radiological examination and follow‐up were conducted according to a standardized protocol. PDL was not removed. Follow‐up periods ranged from 0.2 to 20 years (mean 5.1 years). The risk of ankylosis was analysed using the Cox regression model including stage of root development, length of dry time and age > 20 years. Results The risk of ankylosis significantly increased with increasing length of dry time (hazard ratio: 1.22, P = .002) and was significantly higher for teeth with mature root development than for teeth with immature root development (hazard ratio: 2.1 P < .0001). The estimated risks of ankylosis after 900 days for teeth with immature root development were: dry time < 20 minutes: 33.9% (95% confidence interval (CI): 25.4‐42.7), dry time 20‐40 minutes: 49.6% (95% CI: 37.5‐64.8), dry time 40‐60 minutes: 54.4% (95% CI: 37.1‐71.3) and dry time > 60 minutes: 60.0% (95% CI: 45.0‐75.8). The risks for teeth with mature root development were: dry time < 20 minutes: 59.4% (95% CI: 51.6‐66.5), dry time 20‐40 minutes: 78.3% (95% CI: 69.5‐86.2), dry time 40‐60 minutes: 81.9% (95% CI: 67.2‐92.4) and dry time > 60 minutes: 86.4% (95% CI: 79.7; 92.9). Conclusion The risk of ankylosis rose with increasing length of dry time. However, some teeth may heal without ankylosis even after 60 minutes of dry time. Immature teeth have a lower risk of developing ankylosis. Replantation should therefore always be considered for avulsed teeth.

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Critical appraisal tools used in systematic reviews of in vitro cell culture studies: A methodological study

Barbosa

Silveira

Santos

et al. 2023

Research Synthesis Methods

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Systematic reviews (SRs) of preclinical studies are marked with poor methodological quality. In vitro studies lack assessment tools to improve the quality of preclinical research. This methodological study aimed to identify, collect, and analyze SRs based on cell culture studies to highlight the current appraisal tools utilized to support the development of a validated critical appraisal tool for cell culture in vitro research. SRs, scoping reviews, and meta‐analyses that included cell culture studies and used any type of critical appraisal tool were included. Electronic search, study selection, data collection and methodological quality (MQ) assessment tool were realized. Further, statistical analyses regarding possible associations and correlations between MQ and collected data were performed. After the screening process, 82 studies remained for subsequent analysis. A total of 32 different appraisal tools were identified. Approximately 60% of studies adopted pre‐structured tools not designed for cell culture studies. The most frequent instruments were SYRCLE (n = 14), OHAT (n = 9), Cochrane Collaboration's tool (n = 7), GRADE (n = 6), CONSORT (n = 5), and ToxRTool (n = 5). The studies were divided into subgroups to perform statistical analyses. A significant association (OR = 5.00, 95% CI = 1.54–16.20, p = 0.008) was found between low MQ and chronic degenerative disorders as topic of SR. Several challenges in collecting information from the included studies led to some modifications related to the previously registered protocol. These results may serve as a basis for further development of a critical appraisal tool for cell culture studies capable of capturing all the essential factors related to preclinical research, therefore enhancing the practice of evidence‐based.

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Evaluation of periodontal ligament cell viability in different storage media based on human PDL cell culture experiments—A systematic review

Abstract: Milk remains the most convenient, cheapest, and readily available solution in most situations while also being capable of keeping PDL cells alive. Further studies are required to evaluate the efficacy of more commonly found storage media besides milk.

Cited by 40 publications

References 26 publications

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Risk of ankylosis of 400 avulsed and replanted human teeth in relation to length of dry storage: A re‐evaluation of a long‐term clinical study

Critical appraisal tools used in systematic reviews of in vitro cell culture studies: A methodological study

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