Evaluation of Patient-Administered Tampon Specimens for Chlamydia trachomatis and Neisseria gonorrhoeae

Tabrizi, S N; Fairley, Christopher K; Chen, Shujun; Giouzeppos, Olivia; Paterson, Barbara; Bowden, Francis J.; Garland, Suzanne M.

doi:10.1097/00007435-200003000-00002

Cited by 26 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The three COBAS Amplicor-positive and omp1 PCR-negative anal samples all contained adequate amplifiable DNA and a lack of inhibitors, as shown by ␤-globin detection (14,21,22). It is most probable that these samples were not false positives, but rather contained bacterial levels that were below the detection level of the omp1 PCR assay.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Validation of Roche COBAS Amplicor Assay for Detection of Chlamydia trachomatis in Rectal and Pharyngeal Specimens by an omp1 PCR Assay

et al. 2004

View full text Add to dashboard Cite

Screening guidelines for men who have sex with men (MSM) recommend testing of extragenital sites (pharyngeal and rectal) for gonorrhoea and chlamydia. Testing of specimens from these sites is not validated by most commercial nucleic amplification tests, such as the COBAS Amplicor assay. To investigate the utility of the COBAS Amplicor assay for detection of Chlamydia trachomatis in extragenital specimens, this study developed and evaluated confirmatory tests using the omp1 gene as an alternative target for amplification by PCR. Of anal and throat swabs collected from men in male-only saunas, 52 swabs that tested C. trachomatis positive by COBAS Amplicor and 30 swabs that tested as negative were included for confirmatory omp1 PCR testing. A total of 49 (94%) COBAS Amplicor-positive samples were confirmed by the omp1 PCR. A substantial proportion of specimens were confirmed by using a nested omp1 PCR (27%). Not confirmed by any omp1 PCR were three anal swabs (6%). It is most probable that these samples contained lower bacterial levels that were near or below the detection level of the omp1 PCR assays. The findings of this study support the confident reporting of C. trachomatis detected by COBAS Amplicor in extragenital specimens and support the utility of this assay as a screening test for MSM.

show abstract

Section: Discussionmentioning

confidence: 95%

“…DNA was isolated directly from the clinical samples by using the automated MagNA Pure LC (Roche Diagnostics), and the COBAS Amplicor PCR assay was performed as previously described (21). An additional PCR for amplification of ␤-globin sequence was conducted for detection of inhibition and sample adequacy (22).…”

Section: Methodsmentioning

confidence: 99%

Validation of Roche COBAS Amplicor Assay for Detection of Chlamydia trachomatis in Rectal and Pharyngeal Specimens by an omp1 PCR Assay

et al. 2004

View full text Add to dashboard Cite

show abstract

“…por VR typing is not designed to be a diagnostic test per se but rather a method that can be used in conjunction with NAAT to characterize strains in individuals who have been identified as having gonococcal infections. Several studies have shown that endocervical and vaginal samples are more sensitive than urine for detecting gonococci in women (6,15,17,23,26), and the use of self-collected vaginal swabs might be a viable alternative (17,26). In studies where urine samples are collected, centrifuging the samples and storing the pellets alone may improve subsequent genotypic analyses.…”

Section: Vol 43 2005mentioning

confidence: 99%

“…NAAT are more sensitive than culture (32) and are used to test noncultured samples such as urine, vaginal swabs, and tampons (15,23,26). Therefore, NAAT can be used to easily screen individuals, including those who are asymptomatic, without the need for a physical exam.…”

mentioning

confidence: 99%

Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections

et al. 2005

View full text Add to dashboard Cite

Molecular methods that characterize the Neisseria gonorrhoeae porin protein Por are needed to study gonococcal pathogenesis in the natural host and to classify strains from direct clinical samples used with nucleic acid amplification-based tests. We have defined the capabilities of por variable region (VR) typing and determined suitable conditions to apply the method to direct clinical specimens. Nested PCR from spiked urine samples detected 1 to 10 copies of template DNA; freezing spiked whole urine greatly reduced the ability to amplify porB. In a laboratory model of mixed gonococcal infections, the por type of one strain could be determined in the presence of a 100-fold excess of another. por VR typing was used to examine clinical samples from women enrolled in studies conducted in Baltimore, Md., and Madagascar. por type was determined from 100% of paired cervical swab and wick samples from 20 culture-positive women from Baltimore; results for eight individuals (40%) suggested infection with more than one strain. In frozen urine samples from Madagascar, porB was amplified and typed from 60 of 126 samples from ligase chain reaction (LCR)-positive women and 3 samples from LCR-negative women. The por VR types of 13 samples (21%) suggested the presence of more than one gonococcal strain. Five por types, identified in >45% of women with typed samples, were common to both geographic areas. Molecular typing is an important adjunct to nucleic acid amplificationbased diagnostics. Methods that utilize direct clinical samples and can identify mixed infections may contribute significantly to studies of host immunity, gonococcal epidemiology, and pathogenesis.

show abstract

“…More recently, detection of chlamydial infection by these techniques has also been assessed on secretions recovered from vaginal introitus collected by clinicians or patients themselves (2,5,9,15,16,22,26,31,33) or from vaginal tampons (13,(23)(24)(25). Urine and self-collected samples represent easy and noninvasive techniques compared to the usual cervical swabbing, which necessitates vaginal speculum insertion and a gynecologic examination.…”

mentioning

confidence: 99%

Evaluation of a Modified Sanitary Napkin as a Sample Self-Collection Device for the Detection of Genital Chlamydial Infection in Women

Alary¹,

Poulin²,

Bouchard³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

A modified sanitary napkin was compared with endocervical swab and urine specimens for the detection of urogenital Chlamydia trachomatis infection. Endocervical swabs and/or first-catch urine were collected from 510 women at medical or community settings in Quebec City. Participants were also asked to wear a modified sanitary napkin (Ezy-Detek) during 4 consecutive hours and to bring it back to the clinic or mail it to the laboratory. Endocervical and urine specimens were tested using the Cobas Amplicor CT/NG assay (Roche Diagnostic Systems) according to the manufacturer's instructions, as were specimens collected with the napkin after adequate preparation. If the PCR test result was positive on the endocervical sample or on any two samples, a woman was considered to be infected. PCR testing results on paired samples were identical for 493 Chlamydia trachomatis infection is the most frequently reported sexually transmitted disease in the industrialized countries. The association of this infection with transmission of human immunodeficiency virus infection, pelvic inflammatory disease, infertility, ectopic pregnancy, and chronic pelvic pain (10, 20, 30) constitutes a major public health concern. Because most infected women have no specific symptoms or are asymptomatic, screening programs represent one of the main approaches to control this infection (20,21).The current literature provides very good information on the value of gene amplification techniques, such as PCR or ligase chain reaction, for the detection of C. trachomatis in the lower genital tracts of women. Both technologies have been evaluated in depth using endocervical swab or urine samples. With a specificity higher than 98%, the sensitivity of PCR on either cervical specimens or urine samples varies from 80.0 to 100% (4,12,18,19,27,28).More recently, detection of chlamydial infection by these techniques has also been assessed on secretions recovered from vaginal introitus collected by clinicians or patients themselves (2,5,9,15,16,22,26,31,33) or from vaginal tampons (13,(23)(24)(25). Urine and self-collected samples represent easy and noninvasive techniques compared to the usual cervical swabbing, which necessitates vaginal speculum insertion and a gynecologic examination. In a study, as many as 60% of chlamydia-infected adolescents would have been missed if the urine screening program had not been in place, because they refused the gynecologic examination (14). In addition, specimens may be collected in nontraditional medical settings, such as community centers and schools, and eventually at home (6,11,15,17). Although these methods have been shown to be relatively easy to use and sensitive for the detection of C. trachomatis, some women failed to collect adequate specimens or did not feel at ease with the technique (2,16,31). The objective of the present study was to evaluate a modified sanitary napkin (Ezy-Detek [EDI] Inc., Sillery, Canada) as a specimen self-collection device for detection of chlamydial infection in women. Results of PCR tests ...

show abstract

Evaluation of Patient-Administered Tampon Specimens for Chlamydia trachomatis and Neisseria gonorrhoeae

Cited by 26 publications

References 17 publications

Validation of Roche COBAS Amplicor Assay for Detection of Chlamydia trachomatis in Rectal and Pharyngeal Specimens by an omp1 PCR Assay

Validation of Roche COBAS Amplicor Assay for Detection of Chlamydia trachomatis in Rectal and Pharyngeal Specimens by an omp1 PCR Assay

Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections

Evaluation of a Modified Sanitary Napkin as a Sample Self-Collection Device for the Detection of Genital Chlamydial Infection in Women

Contact Info

Product

Resources

About