Evaluation of parenteral vaccination methods with glycoproteins against Aujeszky's disease in pigs

Vanderpooten, Anita; Goddeeris, Bruno; Roose, P. de; Hendrickx, Louise Antonia; Biront, P.; Desmettre, P

doi:10.1016/s0378-1135(96)01300-4

Cited by 12 publications

(11 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason is not clear, but the i.m. injection occurred at a different place (musculus gastrocnemius compared to musculus gluteobiceps) and it has been observed in previous experiments with pigs comparing immunizations in the back versus the neck that the injection site can influence the isotype profile of the antibody response [43]. Therefore, in contrast to the serum IgA response, the steroid hormone does not enhance consistently the serum IgM response in pigs.…”

Section: Discussionmentioning

confidence: 99%

1α, 25-dihydroxyvitamin D3 increases IgA serum antibody responses and IgA antibody-secreting cell numbers in the Peyer's patches of pigs after intramuscular immunization

Stede

Verfaillie

Cox

et al. 2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARY Pigs were injected intramuscularly (i.m.) twice with human serum albumin (HSA) with or without 1α,25‐dihydroxyvitamin D3[1α,25(OH)2D3] with a 5‐week interval. The supplementation of 1α,25(OH)2D3 enhanced the HSA‐specific IgA serum antibody response but decreased the IgM, IgG, IgG1 and IgG2 responses. Furthermore, higher numbers of HSA‐specific IgA antibody‐secreting cells were obtained in systemic lymphoid tissues (local draining lymph node, spleen and bone marrow) as well as in Peyer's patches and lamina propria of the gut (GALT). In addition, the in vivo mRNA expression for Th1 [interferon (IFN)‐γ, interleukin (IL‐2)], Th2 (IL‐4, IL‐6 and IL‐10) and Th3 [transforming growth factor (TGF)‐β] cytokines as well as the percentage of different cell subsets (CD2+, CD4+, CD8+, IgM+, MHC II+, CD25+) of monomorphonuclear cells from the local draining lymph node were determined at different time‐points after the i.m. immunizations. Cytokine profiles did not resemble a typical Th‐cytokine profile using 1α,25(OH)2D3: higher levels of IL‐10 and significantly lower levels of IL‐2 were observed the first day after the primary immunization. However, significantly higher levels of IL‐2 and significantly lower levels of IFN‐γ were observed the first day after the second immunization. Furthermore, after the second immunization TGF‐β mRNA expression decreased more quickly in the 1α,25(OH)2D3 group. This difference became significant 7 days after the second immunization. One week later a significantly higher percentage of CD25+ cells was observed in this group, indicating more activated T and B cells using the steroid hormone. These results suggest that in pigs the addition of 1α,25(OH)2D3 to an intramuscularly injected antigen can enhance the antigen‐specific IgA‐response and prime GALT tissues, but the relation with cytokines and cell phenotype in the local draining lymph node needs further clarification.

show abstract

Section: Discussionmentioning

confidence: 99%

1α, 25-dihydroxyvitamin D3 increases IgA serum antibody responses and IgA antibody-secreting cell numbers in the Peyer's patches of pigs after intramuscular immunization

Stede

Verfaillie

Cox

et al. 2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…Similarly, immunization with a subunit vaccine based on the essential major envelope glycoprotein, gB, was shown to induce protective immunity in pigs [35]. Likewise, more recent studies have verified the ability of purified ADV glycoproteins to induce protective immunity [18,49]. Thus, utilization of one or more of these three ADV envelope glycoproteins for the development and testing of novel vaccines for pigs is warranted.…”

Section: Adv Target Antigens and Protective Immunitymentioning

confidence: 99%

Aujeszky's disease virus: opportunities and challenges

Zuckermann¹

2000

Vet. Res.

View full text Add to dashboard Cite

-Since its description in 1902, Aujeszky's disease (AD) has become one of the most thoroughly examined viral diseases of swine. The causative agent, Aujeszky's disease virus (ADV), is a neurotropic alphaherpesvirus that produces fatal encephalitis in newborn pigs and a milder syndrome in older animals. In several instances this virus has been used as a test case to examine novel vaccine concepts in swine, including the honor of being the first genetically modified vaccine used in the field. Furthermore, the examination of the immune response to infection or vaccination with this virus has revealed important information about the function of the porcine immune system, including evidence on the existence of a dichotomy between the humoral and cellular immune response in swine. This review presents a summary of research where ADV has been a valuable tool for the development of novel vaccines and has provided information to better understand the immune response of swine to infectious agents. Aujeszky's disease virus / immunity / vaccine / T cells / pigRésumé -Virus de la maladie d'Aujeszky : perspectives et défis. Depuis sa description en 1902, la maladie d'Aujeszky est devenue l'une des maladies virales du porc les plus minutieusement étu-diées. L'agent pathogène responsable de la maladie, le virus de la maladie d'Aujeszky, est un alphaherpesvirus neurotrope qui provoque des encéphalites fatales chez le porc nouveau-né, et un syndrome plus modéré chez les animaux plus âgés. À plusieurs reprises, ce virus a été utilisé expérimentalement pour l'étude de nouveaux concepts vaccinaux chez le porc, et a eu l'honneur d'être le premier vaccin génétiquement modifié utilisé sur le terrain. De plus, l'examen de la réponse immunitaire à l'infection ou à la vaccination par ce virus a révélé des informations importantes concernant le fonctionnement du système immunitaire porcin. En particulier, des preuves de l'existence d'une dichotomie entre les réponses immunitaires humorale et cellulaire chez le porc ont été mises en évidence. Cet article de synthèse résume la recherche pour laquelle ADV a été un outil précieux pour le déve-loppement de nouveaux vaccins, et a apporté des informations pour une meilleure compréhension de la réponse immunitaire du porc aux agents infectieux. Vet. Res. 31 (2000) 121-131 121 virus de la maladie d'Aujeszky / immunité / vaccin / cellules T / porc

show abstract

“…In a few studies, minipigs were used as they are regarded as a suitable model when it comes to vaccination through the skin [ 83 ]. It is noted that almost half of the identified papers (n = 57), do not name the pig breed, often referring to it only as commercial breed [ 49 , 51 , 76 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 ,…”

Section: Resultsmentioning

confidence: 99%

Skin-Based Vaccination: A Systematic Mapping Review of the Types of Vaccines and Methods Used and Immunity and Protection Elicited in Pigs

2023

View full text Add to dashboard Cite

The advantages of skin-based vaccination include induction of strong immunity, dose-sparing, and ease of administration. Several technologies for skin-based immunisation in humans are being developed to maximise these key advantages. This route is more conventionally used in veterinary medicine. Skin-based vaccination of pigs is of high relevance due to their anatomical, physiological, and immunological similarities to humans, as well as being a source of zoonotic diseases and their livestock value. We conducted a systematic mapping review, focusing on vaccine-induced immunity and safety after the skin immunisation of pigs. Veterinary vaccines, specifically anti-viral vaccines, predominated in the literature. The safe and potent skin administration to pigs of adjuvanted vaccines, particularly emulsions, are frequently documented. Multiple methods of skin immunisation exist; however, there is a lack of consistent terminology and accurate descriptions of the route and device. Antibody responses, compared to other immune correlates, are most frequently reported. There is a lack of research on the underlying mechanisms of action and breadth of responses. Nevertheless, encouraging results, both in safety and immunogenicity, were observed after skin vaccination that were often comparable to or superior the intramuscular route. Further research in this area will underlie the development of enhanced skin vaccine strategies for pigs, other animals and humans.

show abstract

Evaluation of parenteral vaccination methods with glycoproteins against Aujeszky's disease in pigs

Cited by 12 publications

References 5 publications

1α, 25-dihydroxyvitamin D3 increases IgA serum antibody responses and IgA antibody-secreting cell numbers in the Peyer's patches of pigs after intramuscular immunization

1α, 25-dihydroxyvitamin D3 increases IgA serum antibody responses and IgA antibody-secreting cell numbers in the Peyer's patches of pigs after intramuscular immunization

Aujeszky's disease virus: opportunities and challenges

Skin-Based Vaccination: A Systematic Mapping Review of the Types of Vaccines and Methods Used and Immunity and Protection Elicited in Pigs

Contact Info

Product

Resources

About