Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area

Siqueira, Liliane Maria Vidal; Gomes, Luciana I.; Oliveira, Edward; Oliveira, Eduardo Ribeiro de; Oliveira, Áureo Almeida de; Enk, Martin Johannes; Carneiro, Nídia Figueiredo; Rabello, Ana; Coelho, Paulo Marcos Zech

doi:10.1590/0074-02760140375

Cited by 25 publications

(52 citation statements)

References 17 publications

(28 reference statements)

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“…Due to its specificity, convenience, and low cost, the Kato-Katz technique (3) is the most commonly used tool in the parasitological survey for the diagnosis of schistosomiasis and soil-transmitted helminths in stool samples from individuals living in endemic areas (4) . However, in low-endemicity areas, where most individuals excrete fewer eggs in their feces, the limited sensitivity of the Kato-Katz technique compromises accurate estimation of the prevalence of the disease (2) (5) (6) (7) . Therefore, the development of improved diagnostic methodologies is essential for overcoming the limitations of the Kato-Katz technique.…”

Section: Introductionmentioning

confidence: 99%

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Siqueira

Couto

Taboada

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Schistosomiasis, caused by Schistosoma mansoni, is a public health concern in Brazil. However, the most popular diagnostic method, the Kato-Katz technique, exhibits low sensitivity in low-endemicity areas. We aimed to compare the performance of an immunological assay, the point-of-care circulating cathodic antigen (POC-CCA®) test, with that of two parasitological techniques in a low-endemicity population. Methods: Our study included 141 individuals living in Estreito de Miralta, Minas Gerais, Brazil. Fecal samples were obtained from all participants and analyzed for schistosomiasis using two parasitological techniques: the Kato-Katz technique and the saline gradient technique. Additionally, POC-CCA® strips were utilized for testing urine samples. The results obtained by the different techniques were compared. Results: Analysis of two or 24 slides using the Kato-Katz technique resulted in a positivity rate of 10.6% (15/141) or 19.1% (27/141), respectively. The saline gradient technique yielded a positivity rate of 17.0% (24/141). The prevalence according to both parasitological techniques was 24.1% (34/141). The POC-CCA® test yielded a positivity rate of 22.7% (32/141); however, the positivity rate was merely 2.1% if trace results were considered negative. The agreements observed between POC-CCA® and the parasitological techniques were good (Kappa indexes > 0.64). The POC-CCA® test was more sensitive than the two-slide Kato-Katz technique (p < 0.05) in detecting cases of S. mansoni infection when trace results were considered positive. Conclusions: These findings reinforce the importance of using multiple diagnostic techniques in low-endemicity areas for effective control of disease.

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Section: Introductionmentioning

confidence: 99%

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Siqueira

Couto

Taboada

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…In the present work, 2 slides of one stool sample were prepared for microscopic analysis, as currently recommended by the Brazilian Ministry of Health for schistosomiasis control. Siqueira et al 15 compared Kato-Katz and a molecular method for the diagnosis of schistosomiasis in a low transmission area. The authors performed Kato-Katz with 12 slides per sample as compared to 2 slides per sample.…”

Section: Discussionmentioning

confidence: 99%

Morbidity of schistosomiasis mansoni in a low endemic setting in Ouro Preto, Minas Gerais, Brazil

Marinho

Grobério

Silva

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Despite the advances of disease control programs, severe forms of schistosomiasis are prevalent. The prevalence of the disease in areas frequented by tourists urges for permanent prevention and control. The aim of this study was to describe the morbidity of schistosomiasis in the district of Antônio Pereira, Ouro Preto, Minas Gerais, Brazil. Methods: The proportion of positives was defined by Kato-Katz coproscopy and urinary POC-CCA rapid test. Hepatosplenic form was diagnosed using abdominal ultrasound. Results: Out of 180 participants,97 were examined by Kato-Katz, with 4 (4.1%) being positive. Thirtyfour (22.1%) out of 154 were positive by POC-CCA. Five (2.8%) of 177 examined by ultrasound had hepatosplenic form. One of them had undergone splenectomy. One (0.6%)participant had myeloradiculopathy. Conclusions: Severe forms of schistosomiasis are still prevalent in low endemic areas and should be thoroughly investigated.

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“…A predominância da esquistossomose na população masculina foi observada neste estudo e corrobora com dados publicados por outros autores 13,14,15 . Embora ambos os sexos estejam submetidos às mesmas condições de saneamento básico, os homens geralmente estão ligados às atividades de pesca e agricultura, em contato direto com água, muitas vezes contaminada, sem nenhum tipo de proteção 16 .…”

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Esquistossomose Na Região Norte Do Brasil

Rodrigues¹,

Dias²,

Rosa³

et al. 2017

Rev Pat Tocantins

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Direitos Autorais: © 2017 Rodrigues Junior et al. Este é um artigo de acesso aberto que permite o uso, a distribuição e a reprodução sem restrições em qualquer meio, desde que o autor original e a fonte sejam creditados. Conflito de interesses ABSTRACTSchistosomiasis is an endemic disease in Brazil. It is caused by the worm Schistosoma mansoni, presenting itself as a serious public health problem. However, despite the relevance of the disease, there are few studies about the disease in the northern region of Brazil, so the present study aimed to evaluate the epidemiological characteristics of schistosomiasis in the region between 2012 and 2015. A cross-sectional study of the information collected in the bank of the national health information system, of the Ministry of Health. During this period, there was a trend in the growth of reported cases in the region, with 65,09% observed in males and 34, 91% of the female sex, 71,60% of the brown race, 17,16% of the white race, 71,01% were urban dwellers and 26,63% were rural. In addition, the majority of the population studied was between 20 and 59 years old. According to previously published studies, there is a trend of increasing incidence of human infection by Schistosoma sp. This perspective of growth of reported cases can be observed in the data found by this study, relevant data for providing information for the construction of control and prevention policies.

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Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area

Cited by 25 publications

References 17 publications

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Morbidity of schistosomiasis mansoni in a low endemic setting in Ouro Preto, Minas Gerais, Brazil

Esquistossomose Na Região Norte Do Brasil

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