Evaluation of parameters obtained from the Sysmex XN‐2000 for predicting the recovery of the absolute neutrophil count and platelets after hematopoietic stem cell transplantation

Park, S.-H.; Park, C.-J.; Kim, M.-J.; Han, Min-Young; Lee, B.-R.; Cho, Y.-U.; Jang, Seongsoo; Kwon, Seog Woon; Kim, D.-Y.; Lee, J.-H.; Lee, K.-H.

doi:10.1111/ijlh.12470

Cited by 8 publications

(9 citation statements)

References 6 publications

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“…Percentage of IPF (IPF%) values correlate well with RP% values obtained by thiazole orange staining (8). It has been demonstrated that IPF% values, especially when measured using the XN series, as well as RP% values, are useful to determine platelet turnover in thrombocytopenic disorders, and for predicting platelet recovery after stem cell transplantation (8)(9)(10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice

et al. 2018

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Ultrastructure analysis of immature platelets is difficult because of the lack of a suitable marker and their relatively low concentration in total platelets. We investigated the morphological and optical properties of human immature platelets produced and enriched in immunodeficient mice via human CD34-positive cell administration. Immunodeficient mice were injected with human CD34-positive cells and administered eltrombopag orally for 14 days (eltro-mice). Some of these mice were maintained for 2-3 months (steady-state-mice). Platelets were double-stained with a human CD41 antibody and a nuclear staining dye (Sysmex hematology analyzer XN series reagent), and then analyzed by flowcytometry FCM to identify human immature platelets. Human CD41-positive cells were isolated from citrated blood by magnetic cell sorting with human CD41 antibody, and examined using electron microscopy. Flow cytometric analysis with the XN reagent demonstrated that peripheral blood from eltro-mice had a higher percentage of immature platelet fraction in human platelets than that from steady-state-mice. The geometric mean of XN reagent fluorescence for human platelets, divided with that for mouse platelets, revealed that the ratios in eltro-mice were significantly higher than those in steady-state-mice, thus indicating that immature platelets were highly enriched in eltro-mice. Scanning and transmission electron microscopy revealed that human citrated platelets isolated from eltro-mice tended to be larger (n = 15, p = 0.276) and contained more mitochondria than those isolated from steady-state-mice (n = 10, p = 0.0002). Therefore, an increased number of mitochondria, rather than platelet size, is a distinctive feature of immature platelets.

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Section: Introductionmentioning

confidence: 99%

Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice

et al. 2018

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“…the time between first rise of the prediction parameter and platelet recovery) of 4.0, 4.5 and 5.0 days for PDW, MPV and P-LCR, respectively, was also significantly longer than for IPF (1.5 days) [74]. However, the authors noted a large inter-individual variation between the rise of the aforementioned parameters and platelet recovery and concluded, that IPF was not clinically meaningful to predict platelet recovery to >20.0 × 10 9 /L, which is the critical decision-making level for platelet transfusion [74]. Although earlier studies suggested that reticulated platelets may be used as a parameter to optimize transfusion policies for platelet concentrates in patients undergoing intensive chemotherapy or HPSCT [20,55,71,75], appropriate prospective trials have not been set-up yet.…”

Section: Recovery Of Thrombopoiesis After Cytostatic Therapy and Tranmentioning

confidence: 83%

“…The median time benefit (i.e. the time between first rise of the prediction parameter and platelet recovery) of 4.0, 4.5 and 5.0 days for PDW, MPV and P-LCR, respectively, was also significantly longer than for IPF (1.5 days) [74]. However, the authors noted a large inter-individual variation between the rise of the aforementioned parameters and platelet recovery and concluded, that IPF was not clinically meaningful to predict platelet recovery to >20.0 × 10 9 /L, which is the critical decision-making level for platelet transfusion [74].…”

Section: Recovery Of Thrombopoiesis After Cytostatic Therapy and Tranmentioning

confidence: 92%

“…as well as for absolute IPF (AUC = 0.68, p < 0.0001) and absolute retPLT (AUC = 0.67, p = 0.0002) to predict platelet recovery within the following 2 days [56]. Another recent analysis of 44 allogeneic HPSCT patients demonstrated comparably poor prediction rates of 68.2% for platelet recovery (≥20 × 10 9 /L) by IPF, whereas other platelet parameters as PDW, MPV and platelet lager cell ratio (P-LCR) showed better prediction rates of 88.6%, 93.2% and 93.2%, respectively [74]. The median time benefit (i.e.…”

Section: Recovery Of Thrombopoiesis After Cytostatic Therapy and Tranmentioning

confidence: 97%

“…Intensive cytostatic therapy to treat hematological malignancies and several types of solid tumors as well as hematopoietic progenitor stem cell transplantation (HPSCT) can induce pronounced transient cytopenia and necessitate prophylactic transfusion support. In order to predict recovery of thrombopoiesis and reduce unnecessary platelet transfusions several studies investigated the usefulness of reticulated platelets determined by custom flow cytometry [64][65][66][67] and automated analyzers [20,55,56,[68][69][70][71][72][73][74]. In general, these studies observed a rise of reticulated platelets several days before platelet recovery.…”

Section: Recovery Of Thrombopoiesis After Cytostatic Therapy and Tranmentioning

confidence: 99%

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Reticulated platelets – clinical application and future perspectives

Meintker

Krause

2020

Journal of Laboratory Medicine

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Reticulated platelets are immature platelets freshly released from the bone marrow into the circulation and contain vestigial amounts of ribonucleic acid. Thus, they can serve as an indicator for the activity of thrombopoiesis. Despite the current lack of a standardized reference method, two types of hematology analyzers have incorporated a fully automated measurement of reticulated platelets. The “immature platelet fraction” (IPF; Sysmex XE-/XN-series) has some clinical utility in the differential diagnosis of thrombocytopenia. This is less clear for “reticulated platelets” (retPLT; Abbott CELL-DYN Sapphire/Alinity HQ). The usefulness of these parameters in the prediction of platelet recovery after chemotherapy or stem cell transplantation and as a decision aid for platelet transfusions has not been unequivocally confirmed. Recent findings have shown an association of reticulated platelets with an adverse risk in patients with coronary artery disease and stroke as well as resistance to anti-platelet therapy. Furthermore, a role of reticulated platelets for the prediction of sepsis was indicated. However, validation in larger prospective trials is necessary to establish the clinical benefit of reticulated platelets in these conditions. This review gives an overview of the available analytical methods and summarizes the current knowledge regarding the clinical application of reticulated platelets.

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Evaluation of immature granulocyte parameters in myeloid neoplasms assayed by Sysmex XN hematology analyzer

Liu

Tian

et al. 2022

J Hematopathol

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Immature granulocytes (IGs) have significance for the diagnosis of myeloid neoplasms (MNs). The current study aims to use a hematology analyzer to evaluate the accuracy of IG parameters in MNs. Blood specimens from 388 patients with MN, 524 with non-hematological neoplasms (non-HNs), including 109 patients with inflammation and 68 undergoing G-CSF administration, and 500 healthy control subjects were analyzed. IG parameters was assayed by Sysmex XN-9000 (XN) and compared with manual assessments. A high level of agreement between IG% derived from XN and manual measurements for MN patients (r = 0.828, p < 0.0001) was revealed but only a moderate correlation for acute myeloid leukemia patients (AML; r = 0.597; p < 0.0001). Bland–Altman bias analysis was conducted, and the results showed that differences in IG% from XN and manual analysis for MN patients were considered clinically insignificant. ROC analysis demonstrated a good performance of IG# (AUC = 0.842) and IG% (AUC = 0.885) assessed by XN for MN patients with cut-off values of 0.200 × 109/L and 1.95%, respectively. IG parameters from Sysmex XN analyzer are helpful for screening of MNs even though granulocyte morphological abnormalities may interfere with IG parameter accuracy.

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Evaluation of parameters obtained from the Sysmex XN‐2000 for predicting the recovery of the absolute neutrophil count and platelets after hematopoietic stem cell transplantation

Cited by 8 publications

References 6 publications

Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice

Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice

Reticulated platelets – clinical application and future perspectives

Evaluation of immature granulocyte parameters in myeloid neoplasms assayed by Sysmex XN hematology analyzer

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