Evaluation of Oxidative Stress, Antioxidant and Thyroid Hormone Status in Patients with Diabetes Mellitus

Pasupathi, Palanisamy; Chandrasekar, Viveksandeep Thoguluva; Kumar, Upendra

doi:10.3329/jom.v10i2.2816

Cited by 26 publications

(37 citation statements)

References 28 publications

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“…According to Raza et al, [5] oxidative stress is an important factor in the etiology and pathogenesis of diabetes mellitus. Furthermore, Pasupathi et al, [3] had observed a significant (p < 0.001) decrease in reduced glutathione (GSH) concentration in diabetic erythrocytes compared to control participants. They further averred that a decreased level of GSH was an after effect of increased utilization of the coenzyme for scavenging ROS due to elevated oxidative stress associated with diabetes.…”

Section: Discussionmentioning

confidence: 99%

“…Hyperglycemia is the earliest and primary clinical presentation in diabetic states. [3,44] Studies on the application of nutraceuticals, sourced from spices and other edible plants and their products, for the treatment and management of diabetes mellitus have received attention of several research endeavours. [45] The present study showed evidence of the capacity of ethanol/water extract of A. sativa to reduce serum level of FBS in hyperglycemic rats, which compared reasonably with the standard anti-diabetic drugglibenclamide ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Erythrocyte glutathione S-transferase: GST activity was measured by previously described methods [3,32,3] with minor modifications. [26] The reaction mixture contained 1.0 mL of 0.3 mM phosphate buffer (pH = 6.5), 0.1 mL of 30 mM 1-chloro-2, 4-dinitrobenzene (CDNB) and 1.7 mL of distilled water.…”

Section: Erythrocyte Haemolysate Haemoglobin Concentrationmentioning

confidence: 99%

“…Primarily, overall physiologic distortions prompted by poor control of metabolism in the absence or insufficiency of insulin engender hyperglycemia and associated metabolic disorders. [1,2] Striking consequential effects of prolonged hyperglycemia include changes in the structure and function of macromolecules, [3,4] auto-oxidation of glycated proteins, increased production of reactive oxygen species (ROS), decreased antioxidant defense, increased lipid peroxidation, and associated apoptosis or necrosis occasioned by membrane degeneration. [4,5] Notably, alterations/adjustments in most glycolytic and tricarboxylic acid cycle (TCA) enzyme activities are associated with diabetic states.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

Chikezie¹,

Uwakwe²

2014

Phcog Commn.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Erythrocyte Haemolysate Haemoglobin Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

Chikezie¹,

Uwakwe²

2014

Phcog Commn.

View full text Add to dashboard Cite

“…It contributes for macrovascular and microvascular complications in diabetes [11]. Prolonged exposure to hyperglycemia leads to increased oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

A Study on Antioxidants status in Type 2 Diabetes Mellitus Patients

G.K

Rahman

Patil

2016

Int J of Biomed & Adv Res

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Background: Diabetes mellitus is an endocrinal disorder. Over production of reactive oxygen and nitrogen species thought to be involved in the initiation and development of vascular complications in diabetes. Increased levels of MDA, Vitamin E and Ceruloplasmin are the markers for oxidative stress in type 2 diabetes mellitus. The aim of the present study is to study the Antioxidant status in Type 2 Diabetes patients. Materials & Methods:This, case control study was conducted on 40 subjects. Among them 20 were controls, 20 were known Type 2 Diabetes patients. Patients with T2DM with other endocrinal disorders, patients on antioxidants, minerals and multivitamins as supplement form are excluded from the study. A laboratory investigations consisting of glucose, total cholesterol, triglycerides, HDLC, LDLC Vitamin E, MDA and Ceruloplasmin were carried out on fasting blood sample of subjects selected for this study. Results: In the present study, FBS, PPBS, MDA, Ceruloplasmin, total cholesterol and LDLC were significantly increased in T2DM cases and HDLC, Vitamin E was significantly decreased in cases when compared to controls. (p value <0.05). Conclusion:The findings of this study is in accordance to earlier studies, that there is an increased oxidative stress in diabetics compared to controls; and the oxidative stress further increases as diabetes to cardiovascular diseases. This study emphasizes the importance of assessing the antioxidant status in diabetes in addition to the markers of oxidative stress and lipid profile to formulate the specific therapies for early therapeutic intervention and better treatment of diabetes mellitus.

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Comparative Evaluation of Cardiac Markers in Differentiated Cells from Menstrual Blood and Bone Marrow-Derived Stem Cells In Vitro

Rahimi

Zarnani

Mohseni-Kouchesfehani

et al. 2014

Mol Biotechnol

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In recent years, menstrual blood-derived stem cells (MenSCs) have been introduced as easily accessible and refreshing stem cell source without ethical considerations in the field of regenerative medicine. The aim of this study was to investigate in vitro cardiac differentiation capacity of MenSCs compared to bone marrow-derived stem cells (BMSCs) under two protocols using 5-aza-2'-deoxycytidine (5-aza) and basic fibroblast growth factor (bFGF). Our data revealed that differentiated MenSCs and BMSCs acquired some features of cardiomyocytes; however, degree of differentiation was dependent on the protocol. In a similar manner with BMSCs, differentiated MenSCs showed upper levels of mRNA/protein of late-stage cardiac markers under 5-aza stimulation and continuous treatment with bFGF (protocol 2) compared to those induced by 5-aza alone (protocol 1) evidencing the key role of bFGF in cardiac development of stem cells. Compared to corresponding undifferentiated cells differentiated MenSCs under protocol 2 showed remarkable expression of connexin-43 and TNNT2 at both gene and protein levels, whereas developed BMSCs under the same condition only expressed connextin-43 at the higher level. Superiority of protocol 2 over protocol 1 was confirmed by assessment of LDH and cTnI production by differentiated cells. Based on the accumulative data, our study provided convincing evidence that MenSCs have relatively higher capability to be differentiated toward cardiomyocyte compared with BMSCs. Furthermore, usage of bFGF and 5-aza to induce in vitro cardiac differentiation of MenSCs is highly recommended.

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Evaluation of Oxidative Stress, Antioxidant and Thyroid Hormone Status in Patients with Diabetes Mellitus

Cited by 26 publications

References 28 publications

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

A Study on Antioxidants status in Type 2 Diabetes Mellitus Patients

Comparative Evaluation of Cardiac Markers in Differentiated Cells from Menstrual Blood and Bone Marrow-Derived Stem Cells In Vitro

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