Evaluation of Oxidative Species in Gaseous and Liquid Phase Generated by Mini-Gliding Arc Discharge

Pawłat, Joanna; Terebun, Piotr; Kwiatkowski, Michał; Tarabová, Barbora; Kovaľová, Zuzana; Kučerová, Katarína; Machala, Zdenko; Janda, M.; Hensel, Karol

doi:10.1007/s11090-019-09974-9

Cited by 71 publications

(45 citation statements)

References 46 publications

(61 reference statements)

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“…The observed concentrations were two orders of magnitude higher than those of 5 mL DI water treated for 1 min with commercially available NEAPPJ operated with He (0.37 mg/L of H2O2, 0.18 mg/L of NO2 − , and 0.06 mg/L of NO3 − ). Additionally, considerably small concentrations of RONS in water treated using NEAPPJ (up to 890 µg/L of H2O2, up to 180 µg/L of NO2 − , and up to 410 µg/L of NO3 − ) [20][21][22]45,46] and custom plasma jets operated with air or addition of air (up to 9.3 mg/L of H2O2, up to 4 mg/L of NO2 − , and up to 5.5 mg/L of NO3 − ) [14,28,47,48] were reported elsewhere, confirming the high efficiency of the developed AALCA as compared with commonly used nonequilibrium plasma jets.…”

Section: Inactivation Of E Colimentioning

confidence: 99%

Direct Treatment of Liquids Using Low-Current Arc in Ambient Air for Biomedical Applications

et al. 2019

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In this work, we developed a portable device with low production and operation costs for generating an ambient air low-current arc (AALCA) that is transferred to the surface of a treated liquid. It was possible to generate a stable discharge, irrespective of the conductivity of the treated liquid, as a sequence of corona, repeating spark, and low-current arc discharges. The estimated concentration of reactive oxygen and nitrogen species (RONS) in plasma-treated water (PTW) produced using AALCA treatment was two orders of magnitude higher than that of PTW produced using conventional He nonequilibrium atmospheric pressure plasma jets or dielectric barrier discharges. The strong bactericidal effect of the treatment using AALCA and the water treated using AALCA was confirmed by survival tests of Escherichia coli. Further, the possibility of treating a continuous flow of liquid using AALCA was demonstrated.

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Section: Inactivation Of E Colimentioning

confidence: 99%

Direct Treatment of Liquids Using Low-Current Arc in Ambient Air for Biomedical Applications

et al. 2019

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“…Atmospheric pressure plasma was generated in GAD reactor presented in Figure 9 developed at Lublin University of Technology (LUT), consisting of two copper electrodes (1.5 mm thick, and 10 cm long with 12° angle between them), which has been described in detail in other related publications [ 15 , 48 ]. The smallest inter-electrode distance was 3 mm.…”

Section: Methodsmentioning

confidence: 99%

Positive Effect of Cold Atmospheric Nitrogen Plasma on the Behavior of Mesenchymal Stem Cells Cultured on a Bone Scaffold Containing Iron Oxide-Loaded Silica Nanoparticles Catalyst

Przekora

Audemar

Pawłat

et al. 2020

IJMS

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Low-temperature atmospheric pressure plasma was demonstrated to have an ability to generate different reactive oxygen and nitrogen species (RONS), showing wide biological actions. Within this study, mesoporous silica nanoparticles (NPs) and FexOy/NPs catalysts were produced and embedded in the polysaccharide matrix of chitosan/curdlan/hydroxyapatite biomaterial. Then, basic physicochemical and structural characterization of the NPs and biomaterials was performed. The primary aim of this work was to evaluate the impact of the combined action of cold nitrogen plasma and the materials produced on proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (ADSCs), which were seeded onto the bone scaffolds containing NPs or FexOy/NPs catalysts. Incorporation of catalysts into the structure of the biomaterial was expected to enhance the formation of plasma-induced RONS, thereby improving stem cell behavior. The results obtained clearly demonstrated that short-time (16s) exposure of ADSCs to nitrogen plasma accelerated proliferation of cells grown on the biomaterial containing FexOy/NPs catalysts and increased osteocalcin production by the cells cultured on the scaffold containing pure NPs. Plasma activation of FexOy/NPs-loaded biomaterial resulted in the formation of appropriate amounts of oxygen-based reactive species that had positive impact on stem cell proliferation and at the same time did not negatively affect their osteogenic differentiation. Therefore, plasma-activated FexOy/NPs-loaded biomaterial is characterized by improved biocompatibility and has great clinical potential to be used in regenerative medicine applications to improve bone healing process.

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“…In order to establish the experimental conditions, preliminary studies were carried out to assess the efficacy of cold plasma inactivation of L. hilgardii cells using various critical plasma parameters such as the type of plasma generator (with different configurations and power supplies) and the type of gas used. For the verification tests, two electrode gliding arc (GAD) reactor 27 , 28 , radio-frequency (RF) plasma jet 29 , 30 , dielectric barrier discharge (DBD) plasma jet with two ring electrodes on the ceramic tube 31 , 32 and DBD plasma jet with one ring electrode on the ceramic tube and grounded electrode below the container were used. After the exposure for 2.5 min, the most effective gas mixture was selected and the operating time was extended to 5 min only for this single gas mixture and the selected reactor.…”

Section: Methodsmentioning

confidence: 99%

Morphological and physiological changes in Lentilactobacillus hilgardii cells after cold plasma treatment

Niedźwiedź

Juzwa

Skrzypiec

et al. 2020

Sci Rep

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Atmospheric cold plasma (ACP) inactivation of Lentilactobacillus hilgardii was investigated. Bacteria were exposed to ACP dielectric barrier discharge with helium and oxygen as working gases for 5, 10, and 15 min. The innovative approach in our work for evaluation of bacterial survival was the use in addition to the classical plate culture method also flow cytometry which allowed the cells to be sorted and revealed different physiological states after the plasma treatment. Results showed total inhibition of bacterial growth after 10-min of ACP exposure. However, the analysis of flow cytometry demonstrated the presence of 14.4% of active cells 77.5% of cells in the mid-active state and 8.1% of dead cells after 10 min. In addition, some of the cells in the mid-active state showed the ability to grow again on culture medium, thus confirming the hypothesis of induction of VBNC state in L .hilgardii cells by cold plasma. In turn, atomic force microscopy (AFM) which was used to study morphological changes in L. hilgardii after plasma treatment at particular physiological states (active, mid-active, dead), showed that the surface roughness of the mid-active cell (2.70 ± 0.75 nm) was similar to that of the control sample (2.04 ± 0.55 nm). The lack of considerable changes on the cell surface additionally explains the effective cell resuscitation. To the best of our knowledge, AFM was used for the first time in this work to analyze cells which have been sorted into subpopulations after cold plasma treatment and this is the first work indicating the induction of VBNC state in L. hilgardii cells after exposure to cold plasma.

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Evaluation of Oxidative Species in Gaseous and Liquid Phase Generated by Mini-Gliding Arc Discharge

Cited by 71 publications

References 46 publications

Direct Treatment of Liquids Using Low-Current Arc in Ambient Air for Biomedical Applications

Direct Treatment of Liquids Using Low-Current Arc in Ambient Air for Biomedical Applications

Positive Effect of Cold Atmospheric Nitrogen Plasma on the Behavior of Mesenchymal Stem Cells Cultured on a Bone Scaffold Containing Iron Oxide-Loaded Silica Nanoparticles Catalyst

Morphological and physiological changes in Lentilactobacillus hilgardii cells after cold plasma treatment

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