Evaluation of oxidant and antioxidant status in patients with vitamin B12 deficiency

Guney, Tekin; Alışık, Murat; Akıncı, Sema; Neşeli̇oğlu, Salim; Dılek, İmdat; Erel, Özcan

doi:10.3906/sag-1407-81

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…Other disease phenotypes under investigation in CC designs included cardiovascular disease [55], schizophrenia [56], and chronic myeloproliferative disorder [57]. Otherwise healthy B12-deficient patients were investigated in five studies, consisting of two CC [58,59], one CS [49], and two RS studies [50,52]. Finally, the RCT by Muss et al [48] focused solely on healthy volunteers.…”

Section: Resultsmentioning

confidence: 99%

“…If the median, range, and sample size were reported, mean ± SD were estimated according to Hozo et al [63]. Three studies supported the antioxidant properties of B12 [53,55,59], one does not [58], and one presented unclear results [51]. Due to heterogeneity in the study populations and oxidative stress markers, grouping of absolute results was not possible.…”

Section: Resultsmentioning

confidence: 99%

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Vitamin B12 in Relation to Oxidative Stress: A Systematic Review

2019

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The triage theory posits that modest micronutrient deficiencies may induce reallocation of nutrients to processes necessary for immediate survival at the expense of long-term health. Neglected processes could in time contribute to the onset of age-related diseases, in which oxidative stress is believed to be a major factor. Vitamin B12 (B12) appears to possess antioxidant properties. This review aims to summarise the potential antioxidant mechanisms of B12 and investigate B12 status in relation to oxidative stress markers. A systematic query-based search of PubMed was performed to identify eligible publications. The potential antioxidant properties of B12 include: (1) direct scavenging of reactive oxygen species (ROS), particularly superoxide; (2) indirect stimulation of ROS scavenging by preservation of glutathione; (3) modulation of cytokine and growth factor production to offer protection from immune response-induced oxidative stress; (4) reduction of homocysteine-induced oxidative stress; and (5) reduction of oxidative stress caused by advanced glycation end products. Some evidence appears to suggest that lower B12 status is related to increased pro-oxidant and decreased antioxidant status, both overall and for subclinically deficient individuals compared to those with normal B12 status. However, there is a lack of randomised controlled trials and prospective studies focusing specifically on the relation between B12 and oxidative stress in humans, resulting in a low strength of evidence. Further work is warranted.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Vitamin B12 in Relation to Oxidative Stress: A Systematic Review

2019

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“…Other studies support the hypothesis that Hcy is a prooxydant marker. In fact, the sulfhydryl groups in Hcy were oxidized to a disulfide, catalyzed by the transition metals which product several Oxygen free radicals (OFR) such as hydroperoxides (H 2 O 2 ), and initiates lipid peroxidation responsible of endothelial injury 19,20 .…”

Section: Fig 3: Positive Correlations Among Ihd Patients (Sod-cat Anmentioning

confidence: 99%

“…Positive correlation between Hcy and TBARS may be explained by the adverse effects of hcy witch involve oxidative damage to vascular endothelial cells and oxidative modification of low density lipoprotein, all there lead to atherosclerosis 19,20 .…”

Section: Fig 3: Positive Correlations Among Ihd Patients (Sod-cat Anmentioning

confidence: 99%

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2016

IJPSR

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Ischemic heart disease (IHD) is the major cause of morbid-mortality in most countries. The involvement of oxidative stress (OS) in the development of atherosclerosis, ischemic cardiopathy triggering phenomen, has been investigated. Serum total homocysteine (Hcy) concentration was measured by fluorescence polarization immunoessay. Plasma levels of substances reacting with thiobarbituric acid (TBARS) were carried out by fluorimetric method (Yagi). Erythrocyte activity of superoxide dismutase (SOD) and plasma total antioxidant status (TAS) were determined by a colorimetric method at 505nm and 600nm, respectively. Erythrocyte activity of catalase (CAT) was measured by colorimetric assay (Góth). Glutathione peroxidase (GPx) and the glutathione reductase (GR) erythrocyte activities were determined by a colorimetric method at 340 nm. Our study showed significant elevation of Hcy and TBARS levels in patients compared to controls. While we noted significant decrease in TAS levels, SOD, CAT, GPx and GR activities in patients compared to the control group. We also noted positive correlations between: Hcy-TBARS, SOD-TAS, CAT-TAS, GR-TAS, GPx-TAS, SOD-CAT and GPx-GR. However negative correlations between: Hcy-SOD, Hcy-CAT, Hcy-GPx, Hcy-GR, Hcy-TAS, TBARS-SOD, TBARS-CAT, TBARS-GPx, TBARS-GR and TBARS-TAS were founded. This unsuitability, elevated prooxidant rate parameters and decrease of antioxidant parameters in patients compared to controls allows supporting the hypothesis that OS is involved in the genesis and progression of atherosclerosis. INTRODUCTION: Inappropriate oxidation of biomolecules is a hazard associated with all aerobic life. Harmful oxidation is often mediated by reactive oxygen species (ROS) that are generated by a wide range of biological processes, including mitochondrial respiration and both enzymatic and non-enzymatic chemical reactions 1. The fine balance between ROS and antioxidants-that "neutralize" ROS before their react with cellular components and alter their structure or function 2 is disturbed when excessive amounts of free radicals (FR) are produced or antioxidant capacity is decreased 3 .

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“…Other studies support the hypothesis that Hcy is a pro-oxydant marker. In fact, the sulfhydryl groups in Hcy were oxidized to disulfide, catalyzed by the transition metals which product several OFR (hydroperoxides: H 2 O 2 ), and initiates lipid peroxidation responsible of endothelial injury 11,28 . Erythrocyte SOD activity was significantly decreased in IHD cases wich is in agreement with several studies such as those reported by Almzaiel AJT et al 14,29,30 .…”

Section: Fig 2: Negative Correlations Among Ihd Patients (Hs_crp-tasmentioning

confidence: 99%

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2016

IJPSR

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Ischemic heart disease (IHD) is the major cause of morbidmortality in most countries. The involvement of inflammation and oxidative stress (OS) in the development of atherosclerosis, ischemic cardiopathy triggering phenomen, has been investigated. The high sensitive C-reactive protein (hs_CRP) was measured in serum by immunoenzymatic method. Serum total homocysteine (Hcy) concentration was measured by fluorescence polarization immunoessay. Plasma levels of substances reacting with thiobarbituric acid (TBARS) were carried out by fluorimetric method (Yagi). Erythrocyte activity of superoxide dismutase (SOD) and plasma total antioxidant status (TAS) were determined by a colorimetric method at 505nm and 600nm, respectively. Erythrocyte activity of catalase (CAT) was measured by colorimetric assay (Goth). Our study showed significant elevation of hs_CRP, TBARS and Hcy levels in patients compared to controls. While we noted significant decrease in TAS levels, SOD and CAT activities in patients compared to the control group. We also noted positive correlations between: hs_CRP-Hcy, hs_CRP-TBARS, Hcy-TBARS, SOD-TAS, CAT-TAS and negative correlations between: hs_CRP-SOD, hs_CRP-CAT, hs_CRP-TAS, TBARS-SOD, TBARS-CAT, TBARS-TAS, Hcy-SOD, Hcy-CAT and Hcy-TAS. This unsuitability, elevated pro-oxidant rate parameters (Hcy and TBARS) and decrease of antioxidant parameters (SOD, CAT and TAS), is a consequence of inflammation, one of the mechanisms which cause atherogenesis. So our study supports the hypothesis involvement of inflammation and OS in the genesis and progression of atherosclerosis.

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Evaluation of oxidant and antioxidant status in patients with vitamin B12 deficiency

Cited by 9 publications

References 12 publications

Vitamin B12 in Relation to Oxidative Stress: A Systematic Review

Vitamin B12 in Relation to Oxidative Stress: A Systematic Review

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