Evaluation of Ovaprim and human chorionic gonadotropin doses on spawning induction and egg and larval quality of pinfish, Lagodon rhomboides

DiMaggio, Matthew A.; Broach, Jason S.; Ohs, Cortney L.

doi:10.1016/j.aquaculture.2013.07.012

Cited by 29 publications

(36 citation statements)

References 29 publications

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“…The large spawn (more than double of any other spawn) observed on 21 March may suggest complete egg release may have only occurred once and other spawns could have possibly contained eggs just from a single female. If such were true, not achieving maximum egg release would appear a negative characteristic of protracted non‐induced L. rhomboides spawning, however the batch fecundity estimate in the present experiment was well within the range (12·7–65·0 eggs g −1 female M B ) for L. rhomboides after they have been hormonally induced to spawn (DiMaggio et al, ; Broach et al, ).…”

Section: Discussionmentioning

confidence: 46%

“…5·0%) to metamorphosis for a marine finfish (DiMaggio et al, ). Broodfish can be induced to spawn using commonly available hormones including Ovaprim (Syndel; http://www.syndel.com) and Chorulon (Intervet International; http://www.merck.com) and produce high quality larvae (DiMaggio et al, ). Their most positive culture trait is perhaps their ability to spawn volitionally for a protracted period of time in captivity without hormonal induction (authors, pers.…”

Section: Introductionmentioning

confidence: 99%

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Protracted volitional spawning of pinfish Lagodon rhomboides and changes in egg quality and fatty‐acid composition throughout the spawning season

Broach

Ohs

Breen

2017

Journal of Fish Biology

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Spawning performance of pinfish Lagodon rhomboides without use of hormonal aids was monitored over an extended season. Nearly three million eggs were obtained from 75 spawns collected over a 90-day consecutive period from a single population of four brood fish (1M:1F). A mean ± s.d. batch fecundity of 30·27 ± 22·64 eggs g female was estimated with 98·0 ± 0·06% of the batch composed of floating eggs which were 1·04 ± 0·04 mm in diameter and 85·71 ± 27·59% fertile. Floating eggs successfully hatched 54·65 ± 29·13% of the time which yielded larvae that were 2·59 ± 0·24 mm in length. Fatty acids within floating eggs were largely represented by polyunsaturated fatty acids (45·30 ± 2·14% of total fatty acids) of which linoleic acid [(c18:2n-6cis) 3·49 ± 1·69% trifluoroacetic acid (TFA)] and docosahexaenoic acid (DHA) [(c22:6n-3) 28·47 ± 1·48% TFA] represented the majority of fatty acids for n-6 and n-3 polyunsaturated fatty acids, respectively. The strongest correlations between fatty acids and hatching success and larval survival to first feeding were observed for the DHA:EPA (eicosapentaenoic acid; c20:5n-3) ratio and total n-6 polyunsaturated fatty-acids levels, respectively. These data demonstrate potential for producers to rely on natural spawns for extensive egg production and provide a baseline for future development of natural spawning protocols of captive L. rhomboides.

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Section: Discussionmentioning

confidence: 46%

Section: Introductionmentioning

confidence: 99%

Protracted volitional spawning of pinfish Lagodon rhomboides and changes in egg quality and fatty‐acid composition throughout the spawning season

Broach

Ohs

Breen

2017

Journal of Fish Biology

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“…Pinfish and pigfish larvae at hatch and first feeding were larger and very close to the size reported for each species in literature (Schimmel ; DiMaggio et al . , ), during the static incubation experiments vs. those observed in the other two experiments. This may be a manifestation of differences in the quality of the spawns obtained from the broodfish.…”

Section: Discussionmentioning

confidence: 75%

“…This may be due to differences in metabolism or to the larger size of eggs and embryos for pinfish (egg diameter: ~900 μm) compared to pigfish (egg diameter: ~800 μm) (Cardeilhac ; Schimmel ; Sutter & McIlwain ; DiMaggio et al . , ) and the overall greater biomass of eggs stocked at each density leading to a greater capacity for oxygen consumption and waste production by the developing embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Surveys, both past and recent, demonstrate the strong market potential for cultured pinfish and pigfish sold as bait to supplement wild‐caught sources when seasonal shortages occur (Adams, Lazur, Zajicek & Zimet ). Research on both species has increased in recent years, demonstrating their desirable culture traits including successful juvenile grow out at high densities (Ohs, Grabe, DeSantis, Rhyne & DiMaggio ; Ohs, Grabe, DeSantis, DiMaggio & Rhyne ; DiMaggio, Ohs, Broach & Sink ) and when fed appropriate dietary rations (Oberg, Faulk & Fuiman ), and relative ease of spawning using hormonal aids (DiMaggio, Broach & Ohs , ) and manipulation of photo‐thermal cycles (Jason S Broach, personal observation). Larvae of both species have been successfully cultured in recirculating systems; however, survival for both species to complete metamorphosis into juveniles is low (≤10%; DiMaggio, Grabe, DeSantis & Ohs ; DiMaggio, Broach, Ohs & Grabe ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of egg stocking density on egg hatchability, larval quality and water quality for pinfish, Lagodon rhomboides , and pigfish, Orthopristis chrysoptera

2016

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Pinfish (Lagodon rhomboides Linnaeus) and pigfish (Orthopristis chrysoptera L.) are relatively new, cultured species commonly used as marine baitfish in the south‐eastern United States and currently have no defined protocols for egg incubation. Thus, experiments were conducted to determine efficient egg stocking densities during static and flow‐through incubation that yielded higher quality larvae. Eggs of each species were incubated statically at 250, 500, 1000 and 2000 eggs L−1, and after incubation, egg hatching success, larval survival to first feeding, larval morphometrics and water quality were assessed. Stocking densities above 250 eggs L−1 led to significant reductions in pinfish hatching success and water quality degradation, evident from decreased dissolved oxygen and pH levels and increased nitrogenous wastes. Increased stocking densities for pigfish also resulted in significant water quality degradation, although hatching success was unaffected up to a density of 1000 eggs L−1. A high flow‐through water exchange rate of 2000% daily resulted in significant reductions in nitrogenous wastes and greater stability in dissolved oxygen and pH levels during incubation when compared to static treatments of the same egg density. Additionally, the high exchange rate of 2000% was critical in maintaining high hatching success and larval survival to first feeding at stocking densities of 1000 eggs L−1 for pinfish and up to 4000 eggs L−1 for pigfish. No clear patterns in larval morphometrics were observed among stocking densities. Static incubation densities of 250 and 1000 eggs L−1 are recommended for pinfish and pigfish, respectively.

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Effects of different spawning agents on serum levels of reproductive steroid hormones and cortisol level in adult female Barbus sharpeyi (Gunther, 1874)

Mohammadian

Malekpouri

Zare

et al. 2015

Fish Physiol Biochem

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The question of whether, as hormone therapies, spawning agents differ from each other to induce physiological pathways of gametogenesis and oocyte maturation in fish remains important, because it could modify undesirable changes, regulated by endocrine systems of individual fish. A series of experimental treatments were applied to investigate the underlying mechanism(s) in which female bunnei (Barbus sharpeyi) fish respond differently to hormone therapies. Female broodstocks were injected twice (with 12 h interval) by three different treatments namely A, B and C. The treatment A received carp pituitary extract (CPE) + luteinizing hormone-releasing hormone analogs (LHRHα2) (0.5 mg CPE kg(-1) BW for first injection and 2 mg CPE kg(-1) BW + 10 µg LHRHα2 kg(-1) for second injection), treatment B received CPE (0.5 and 3.5 mg kg(-1) BW), and treatment C received ovaprim (0.1 and 0.15 ml kg(-1) BW). Blood samples were collected at four different time intervals, including prior to injections, 6 h after first injection, 6 h after second injection and at the time of spawning, and serum steroid hormones, including testosterone, progesterone and estradiol-17β as well as cortisol, were measured. Results showed significant increases in serum estradiol-17β following all treatments, but the most profound response was found in treatments A and B. Testosterone was higher in larger broodfish than in small-sized broodfish (>1.5 vs. <1.5 kg) in all treatments. CPE led to higher concentration of testosterone rather than two other treatments. CPE also increases the progesterone following first injection and approximately remains unchanged till the end of experiment. Change in progesterone level was only significant after second injection of ovaprim as well as after spawning compared with previous time. Linear regression analyses indicated that cortisol had adverse effects on progesterone and testosterone levels of weight group <1.5 kg. These results suggest that among inducing agents, applied here, CPE can provide more reasonable response in reproduction of female B. sharpeyi.

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Evaluation of Ovaprim and human chorionic gonadotropin doses on spawning induction and egg and larval quality of pinfish, Lagodon rhomboides

Cited by 29 publications

References 29 publications

Protracted volitional spawning of pinfish Lagodon rhomboides and changes in egg quality and fatty‐acid composition throughout the spawning season

Protracted volitional spawning of pinfish Lagodon rhomboides and changes in egg quality and fatty‐acid composition throughout the spawning season

Effects of egg stocking density on egg hatchability, larval quality and water quality for pinfish, Lagodon rhomboides , and pigfish, Orthopristis chrysoptera

Effects of different spawning agents on serum levels of reproductive steroid hormones and cortisol level in adult female Barbus sharpeyi (Gunther, 1874)

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