Evaluation of osteointegrative and osteoinductive properties of silicon doped ceramics in a model of rabbit’s femur fractures

Abstract

Bone regeneration is one of the most complex and unique types of tissue regeneration, although quite long in time, comparatively, for example, with soft tissues, but provides the complete identity of the damaged site with normal bone. The most complex fractures are fragmentation, which can be occurs within wide range - 25-60% of the total number of all fractures. In such cases, due to the loss of contact with soft tissues, the fragments lose blood supply and regeneration, which leads to different bone size defect. This condition cause limitation of the main mechanisms of bone consolidation – endoostal and intramembrane ossification. In this regard, a strategic medical treatment is the replacement of bone defect with biological or synthetic material, which creates a site for the processes of reparative osteogenesis. The most widespread combined biocompatible materials in the various combinations of β-tricalcium phosphate and hydroxyapatite ("Maxresorb®", "Perossal®", "calc-i-oss®CRYSTAL", "easy-graft®CRYSTAL"), or composite composites based on bioactive and biogenic materials: hydroxylapatite + collagen (Biostite, Collagraft, Avitene, Collola, Hapkol, Collapan, MP Composite); hydroxylapatite + tricalcium phosphate + collagen ("Hydroxyapol", "Collapolum"); hydroxylapatite + collagen + sulfated glycosaminoglycans ("Biomatrix", "Osteomatrix", "Bioimplant"). Unfortunately, in veterinary medicine osteotropic materials developed for humane medicine are used only. Recently, a separate group of biocompatible composites based on the combination of hydroxyapatite with β-tricalcium phosphate, doped with magnesium, sodium, potassium, zinc, copper, aluminum, strontium, silicon, germanium, in order to provide them with specific properties - antibacterial, osteoinductive, antitumor, immunomodulating, etc. However, the spectrum of biological effects of these ions on bone metabolism is extremely diverse, and therefore the use of composite ceramics doped with microelement ions requires a comprehensive clinical and experimental justification. The purpose of the study is to evaluate the osteointegration and osteoinductive properties of ceramics based on hydroxyapatite and β-tricalcium phosphate doped with silicon for model fractures of the femur in rabbits. The work is done on rabbits of Californian breed at the age of 3 months. and a weight of about 2.5 kg. To substantiate the ceramics GTlKg-2, 2 groups of 10 rabbits were formed in each, in which model bone defects were formed in the distal parts of the hip dysthymia. Animals of the experimental group defects filled with granules of ceramics. In the rabbits of the control group, the defect was left to heal under a blood clot. Animals were extracted from the experiment at the 21st and 42nd day. X-ray and histomorphological studies were performed. On the 21st day of reparative osteogenesis, rabbits of all groups fully rested on the injured limb, signs of inflammatory reaction were absent in the experimental group, and the control marked the pronounced seal of the periosteum across the entire surface of the femur. It should be noted that hydroxyapatite ceramics does not possess x-ray contrast properties. On the 42nd day of regeneration of rabbits both groups fully rested on injured limb, signs of inflammatory reaction of soft tissues in the area of injury were absent. Radiologically, in animals of the experimental group in the place of bone defect, spot osteosclerosis was detected in the form of a clearly defined white heel, opposite to which the contour of the periosteum was sealed. At the same time, on the control X-rays, along with a well-defined, but more elongated septum of the periodontal, revealed a bone marrow panossus at the site of the injury, with a clearly defined extension of the eclipse. Substantially complemented macromorphological picture of bone biopsy. In particular, in the case of replacement of bone defect GTlKg-2, at the 21st day in the traumatic areas a limited and moderate periosteal reaction was noted. Along with this, in control animals, in this period, it was not completely replaced by fibrous cartilaginous tissue, as evidenced by its craterial appearance. Histologically, in the control animals, the bone defect formed a cartilage tissue along the periphery, and the bone beams, which were at a certain distance from the place of the defect, were at the stage of resorption. In the case of its replacement granules GTlKg-2 formed bone-ceramic regenerate, that is, the intervals between the granules are filled with bone tissue. The obtained results give grounds to consider that GTlKg-2 contributes to the formation of bone tissue due to its osteointegration and osteoinductive properties. Key words: reparative osteogenesis, osteointegration, osteocytes, osteoblasts, hydroxyapatite composite with β-tricalcium phosphate, doped with silicon.

Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator

“…Репаративний остеогенез як складний і багатокомпонентний, генетично запрограмований біологічний процес, лежить в основі відновлення структурної організації та цілісності кісткової тканини після травми. Ключовими ланцюгами остеорепаративного процесу є резорбція через запалення пошкодженої тканини, диференціювання клітин, їх проліферація та утворення нової кістки з її ремоделюванням під дією біомеханічних факторів, формування органічного позаклітинного матриксу, його мінералізація (Diedukh & Nikolchenko, 2009;Gajko & Brusko, 2013;Rublenko et al, 2018).…”

“…У випадку надання їм остеоіндуктивних властивостей вони потребують оцінки не тільки місцевої, а й загальної реакції організму. Зокрема, як було вказано в ряді робіт (Rublenko et al, 2018;Chemerovskyi, 2020), за використання гідроксиапатиту, легованого кремнієм, репаративний остеогенез прискорюється за рахунок скорочення запально-резорбтивної стадії та прискорення проліферативної, головним чином з боку ендоосту з ранньою мінералізацією кісткового регенерату. При цьому динаміка гематологічних показників є типовою для консолідації довгих трубчастих кісток, що свідчить про відсутність вираженої загальної реакції організму на імплантацію гідроксиапатитної кераміки, легованої кремнієм.…”

X-ray and macromorphological evaluation of reparative osteogenesis by implantation of hydroxyapatite composite doped with germanium

“…Найбільший її рівень у собак і котів -6-15 %, менший у коней -7 %, досить низький -1,6-1,8 % у жуйних і свиней [2]. При цьому серед травм локомоторного апарату в тварин-компаньйонів досить велику частку -25-60 %, займають осколкові переломи довгих трубчастих кісток, які є найбільш складними і потребують комплексного підходу в лікуванні [6,7], оскільки їх консолідація у 7-32 % випадків ускладнюється різними формами незрощень, псевдосуглобами, контрактурами чи остеомієлітами.…”