Evaluation of Osseointegration and Bone Healing Using Pure-Phase β - TCP Ceramic Implant in Bone Critical Defects. A Systematic Review

García, Daniel; Mingrone, Larissa Eckmann; Sá, Marcelo Jorge Cavalcanti de

doi:10.3389/fvets.2022.859920

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…It takes 3-6 months for bone trabeculae to be reconstructed at the site of the bone tissue defect (Garcia et al, 2022). To study the stability of the met/α-CSH/nHA materials, degradation, and metformin release experiments were carried out.…”

Section: Resultsmentioning

confidence: 99%

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Liu

Fu²,

Lv³

et al. 2022

Front. Bioeng. Biotechnol.

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This study aimed to examine the effects of loading different concentrations of metformin onto an α-hemihydrate calcium sulfate/nano-hydroxyapatite (α-CSH/nHA) composite. The material characteristics, biocompatibility, and bone formation were compared as functions of the metformin concentration. X-ray diffraction results indicated that the metformin loading had little influence on the phase composition of the composite. The hemolytic potential of the composite was found to be low, and a CCK-8 assay revealed only weak cytotoxicity. However, the metformin-loaded composite was found to enhance the osteogenic ability of MC3T3-E1 cells, as revealed by alkaline phosphate and alizarin red staining, real-time PCR, and western blotting, and the optimal amount was 500 µM. RNA sequencing results also showed that the composite material increased the expression of osteogenic-related genes. Cranial bone lacks muscle tissue, and the low blood supply leads to poor bone regeneration. As most mammalian cranial and maxillofacial bones are membranous and of similar embryonic origin, the rat cranial defect model has become an ideal animal model for in vivo experiments in bone tissue engineering. Thus, we introduced a rat cranial defect with a diameter of 5 mm as an experimental defect model. Micro-computed tomography, hematoxylin and eosin staining, Masson staining, and immunohistochemical staining were used to determine the effectiveness of the composite as a scaffold in a rat skull defect model. The composite material loaded with 500 µM of metformin had the strongest osteoinduction ability under these conditions. These results are promising for the development of new methods for repairing craniofacial bone defects.

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Section: Resultsmentioning

confidence: 99%

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Liu

Fu²,

Lv³

et al. 2022

Front. Bioeng. Biotechnol.

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“…That is, only the presence of a sufficient amount of bone tissue in the region of the alveolar ridge makes the favourable prognosis for orthopedic treatment with dental implant placement. To date, dentistry has accumulated sufficient experience in the development and implementation of various osteoplastic techniques for guided tissue regeneration, in particular, in dental implantation, o in order to restore the amount of the jaw bone, taking into account the specific clinical situation [6,7].…”

Section: Introductionmentioning

confidence: 99%

Experimentall Substantiation for Applying Actovegin in Combination With Osteoplastic Materials in Mandibular Bone Tissue Regeneration

Hefzollesan

Mammadov

Arkhmamаdova

et al. 2022

Act. Probl. of the Modern Med.

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The aim of the study is to perform a comparative assessment of the effectiveness of applying various osteoplastic materials in combination with Actovegin in stimulating bone tissue regeneration. Materials and methods. In order to study the osteoplastic properties and determine the peculiarities of the course of reparative processes, as well as to substantiate the treatment method we elaborated, in vivo experimental studies were carried out on 36 male rabbits weighing from 1.5 to 2.5 kg at the Scientific Research Centre of the Azerbaijan Medical University. The animals were kept in a vivarium under standard conditions on the standard diet and were under the study for 60 days since the inclusion in the experimental model. Having been included into the study, the animals were randomly divided into 3 groups of 12 rabbits in each: in the animals of Group I, the main group, individual bone defects were formed on both sides of the lower jaw; the subgroup A included animals, whose artificially formed defects on one side of the jaw were closed with an allograft and platelet-rich fibrin exudate (PRF), subgroup B included animals with the same modelled damage, who received the treatment mentioned above and additional Actovegin; Group II was a comparison group, received a mixture of autogenous bone, PRF and Actovegin injected into jaw defects; Group III was the control group, which received PRF and Actovegin injected intravenously in a dose of 1 mg daily for 2 weeks. Results. Neutrophilic infiltration was found at the maximum level (1.33±1.07) in the area of the mandibular bone defect in subgroup A of I group, where only the allograft was used without additional Actovegin. In group III, who received the combination with PRF and intravenous administration of Actovegin, the average rate of lymphocytic infiltration by the end of the experiment was 1.75±0.75 that correlates with the severity of the degree of bone defect closure. Vascularization or blood supply to pre-damaged bone tissue, in particular to the residual area of the bone defect, was detected at the lowest level in the group of animals, who did not receive Actovegin during the implantation of osteotropic material into the defect site (1.25±0.45). Vascularization, which impacts the bone tissue regeneration, was the most intensive when applying Actovegin (1.91±0.66). In the samples obtained in group II and subgroup B, more capillaries and, accordingly, a high density of the capillary network (p<0.01) were detected in the area of the bone defect more often than in other groups of animals. Conclusion. Inflammatory changes (neutrophilic, lymphocytic and plasma cell infiltration) in the residual zone, which is part of the bone defect, were less pronounced in the group of topical application of autologous bone and Actovegin. At the same time, it was noted that the amount of eosinophilic infiltration and granulomatous reactions were more intense when using an allograft.

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Silver and magnesium co-doped β-TCP nanoparticles as potential materials for bone tissue engineering

Noori,

Khamis,

Shather

et al. 2024

Appl. Phys. A

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Evaluation of Osseointegration and Bone Healing Using Pure-Phase β - TCP Ceramic Implant in Bone Critical Defects. A Systematic Review

Cited by 8 publications

References 15 publications

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Experimentall Substantiation for Applying Actovegin in Combination With Osteoplastic Materials in Mandibular Bone Tissue Regeneration

Silver and magnesium co-doped β-TCP nanoparticles as potential materials for bone tissue engineering

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