Evaluation of optimal water fluoridation on the incidence and skeletal distribution of naturally arising osteosarcoma in pet dogs

Rebhun, Robert B.; Kass, Philip H.; Kent, Michael S.; Watson, Katherine D.; Withers, Sita S.; Culp, William T. N.; King, Aaron A.

doi:10.1111/vco.12188

Cited by 5 publications

(2 citation statements)

References 27 publications

(62 reference statements)

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“…Fluoride is considered one of the most common environmental risk factors, especially in drinking water. 2 The epidemiological evidence of the relationship between fluoride and osteosarcoma risk has been examined in animals [3][4][5] and humans, [6][7][8][9][10][11][12][13][14][15][16][17] with conflicting results. Some studies have reported that fluoride could affect the proliferation or progression of osteosarcoma, while others have declared no obvious correlation between them.…”

Section: Introductionmentioning

confidence: 99%

Integrative analyses of key genes and regulatory elements in fluoride‐affected osteosarcoma

Jin

Guo

et al. 2019

J of Cellular Biochemistry

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Osteosarcoma is one of the most malignant tumors in adolescents with severe outcomes while fluoride is one of the most abundant elements in the environment. Epidemiological evidence has elucidated the relationship between fluoride and osteosarcoma, but the molecular mechanisms are extremely complicated. Microarray profiles were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) in the progression of fluoride‐affected osteosarcoma. The functional enrichment analysis was performed, a protein‐protein interaction network, a microRNA‐messenger RNA (mRNA) and a transcription factors‐mRNA regulatory network were constructed and performed using Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape. A total of 171 DEGs were identified. The functions and pathways of the DEGs were enriched in nucleolus, protein ubiquitination, protein binding, RNA transport, and the spliceosome. Eighteen hub genes were identified and functional analysis revealed that these genes are mainly enriched in protein binding, nucleoplasm, and ribosomal RNA processing. Survival analysis showed that the hub genes may be involved in the invasion or recurrence of osteosarcoma. In conclusion, the DEGs and hub genes with their regulatory elements identified in this study will help us understand the molecular mechanisms underlying fluoride‐affected osteosarcoma and provide candidate targets for future research.

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Section: Introductionmentioning

confidence: 99%

Integrative analyses of key genes and regulatory elements in fluoride‐affected osteosarcoma

Jin

Guo

et al. 2019

J of Cellular Biochemistry

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“…However, at exposure levels that are generally well above the exposure levels correspond to intentional fluoridation of drinking water [ 16 ]. Additional studies have further supported the presumption that drinking water fluoridation is not associated with an increased risk of osteosarcoma [ 17 , 18 , 19 , 20 , 21 ] nor with altered thyroid function [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

A Physiologically-Based Pharmacokinetic Modeling Approach Using Biomonitoring Data in Order to Assess the Contribution of Drinking Water for the Achievement of an Optimal Fluoride Dose for Dental Health in Children

Jean

Wassef

Gagnon

et al. 2018

IJERPH

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Due to an optimal fluoride concentration in drinking water advised for caries prevention purposes, the population is now exposed to multiple sources of fluoride. The availability of population biomonitoring data currently allow us to evaluate the magnitude of this exposure. The objective of this work was, therefore, to use such data in order to estimate whether community water fluoridation still represents a significant contribution toward achieving a suggested daily optimal fluoride (external) intake of 0.05 mg/kg/day. Therefore, a physiologically-based pharmacokinetic model for fluoride published in the literature was used and adapted in Excel for a typical 4-year-old and 8-year-old child. Biomonitoring data from the Canadian Health Measures Survey among people living in provinces with very different drinking water fluoridation coverage (Quebec, 2.5%; Ontario, 70% of the population) were analyzed using this adapted model. Absorbed doses for the 4-year-old and 8-year-old children were, respectively, 0.03 mg/kg/day and 0.02 mg/kg/day in Quebec and of 0.06 mg/kg/day and 0.05 mg/kg/day in Ontario. These results show that community water fluoridation contributes to increased fluoride intake among children, which leads to reaching, and in some cases even exceeding, the suggested optimal absorbed dose of 0.04 mg/kg/day, which corresponds to the suggested optimal fluoride intake mentioned above. In conclusion, this study constitutes an incentive to further explore the multiple sources of fluoride intake and suggests that a new balance between them including drinking water should be examined in accordance with the age-related physiological differences that influence fluoride metabolism.

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Demographic characteristics, site and phylogenetic distribution of dogs with appendicular osteosarcoma: 744 dogs (2000-2015)

et al. 2019

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ObjectiveTo report demographic characteristics of a contemporary population of dogs with appendicular osteosarcoma and assess the relationship between demographic characteristics, site distribution, and phylogenetic breed clusters.DesignRetrospective case series.MethodsA search of the Veterinary Medical Database was performed for dogs with appendicular osteosarcoma as a new diagnosis. Entries were reviewed for the sex, neuter status, age at diagnosis, breed, affected limb, and tumor location. The reported breed for purebred dogs was used to categorize each dog into one of five phylogenetic groups based on microsatellite analysis.Results744 client-owned dogs were included in the study. Study dogs were represented by a male-to-female ratio of 0.95:1.0, the majority of which (80.9%) were neutered. Most dogs were diagnosed between 7–10 years of age. The majority (77.8%) of dogs were large or giant-breed dogs. Purebred dogs comprised 80.4% of the population. The most common purebred breed affected by OS was the Rottweiler (17.1%). The most common phylogenetic group represented was Mastiff-Terrier (M-T, 26.3%). OS was more commonly located in the forelimb (64.2%) versus the hindlimb (35.8%), and the humerus was the most common site (20.9%). The distribution of age groups and tumor locations were significantly different between phylogenetic clusters. The distribution of age groups and neuter status were significantly different between size groups.Conclusions and significanceThe demographic data of canine appendicular OS are similar to previous reports. The data on phylogenetic associations can guide future studies aimed at evaluating the genomic mutations that contribute to OS development and its biological behavior.

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Evaluation of optimal water fluoridation on the incidence and skeletal distribution of naturally arising osteosarcoma in pet dogs

Cited by 5 publications

References 27 publications

Integrative analyses of key genes and regulatory elements in fluoride‐affected osteosarcoma

Integrative analyses of key genes and regulatory elements in fluoride‐affected osteosarcoma

A Physiologically-Based Pharmacokinetic Modeling Approach Using Biomonitoring Data in Order to Assess the Contribution of Drinking Water for the Achievement of an Optimal Fluoride Dose for Dental Health in Children

Demographic characteristics, site and phylogenetic distribution of dogs with appendicular osteosarcoma: 744 dogs (2000-2015)

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