Evaluation of optimal concentration and exposure duration of valproic acid alone or in combination with ViraDuctin to augment adenovirus transduction in human adipose stem cells

Negahdari, Babak; Khorramizadeh, Mohammad Reza; Modarresi, Mohammad Hosein; Hartoonian, Christine; Shokrghozar, Mohammad Ali; Azadmanesh, Kayhan

doi:10.1016/j.jcyt.2013.08.015

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The hADSCs were isolated from human subcutaneous adipose tissue of patients undergoing surgery with ages ranging from 30 to 50 years, according to the previous study by Negahdari et al [25]. Briefly, adipose tissue was chopped and washed with PBS buffer and then digested with 0.02 mg ml −1 Collagenase Type I (Sigma-Aldrich, Germany) solution under constant shaking for 60 min at 37 °C.…”

Section: Biological Evaluations 251 Hadscs Isolation Seeding and Cult...mentioning

confidence: 99%

Osteogenic potential of Rosuvastatin immobilized on silk fibroin nanofibers using argon plasma treatment

2018

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To begin developing a silk fibroin (SF) nanofibrous scaffolds that could promote osteogenesis, whilst enabling to deliver an active amount of Rosuvastatin (RSV) to the cells in long-time period, the present study aims to immobilize RSV onto the SF nanofibers through the argon radio frequency. Thus, the effect of plasma exposure times (0, 1, 3, and 5 min) was investigated on the morphology, loading efficiency, release profile, and osteogenesis activity. The successful loading of RSV on the SF nanofibers was proved by Fourier transformed infrared spectroscopy, Differential scanning calorimetry, and energy dispersive spectroscopy. In vitro drug release studies demonstrated that the RSV release was prolonged over a period of 21 d for plasma treated mats, while the non-plasma treated samples released the whole drug after 72 h. Moreover, the dose of RSV was controlled by the plasma exposure times, in which the highest amount of the released RSV was achieved after 3 min exposing to plasma. As suggested by MTT assay, the released amounts of RSV had no toxicity on the seeded human adipose tissue-derived stem cells and enhanced their proliferation. Moreover, using real-time reverse transcriptase-polymerase chain reaction and alizarin red staining proved that RSV-immobilized SF mats stimulate both early and late osteogenic gene differentiation in comparison with pure SF nanofibers. However, the highest differentiation was observed on the SF nanofibers treated with argon plasma for 3 min. The results support the potential of plasma treatment on sustained release of the RSV from SF nanofibers for osteogenesis enhancement.

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Section: Biological Evaluations 251 Hadscs Isolation Seeding and Cult...mentioning

confidence: 99%

Osteogenic potential of Rosuvastatin immobilized on silk fibroin nanofibers using argon plasma treatment

2018

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Electrospun core-sheath poly(vinyl alcohol)/silk fibroin nanofibers with Rosuvastatin release functionality for enhancing osteogenesis of human adipose-derived stem cells

Kalani

Nourmohammadi

Negahdari

et al. 2019

Materials Science and Engineering: C

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Evaluation of optimal concentration and exposure duration of valproic acid alone or in combination with ViraDuctin to augment adenovirus transduction in human adipose stem cells

Cited by 2 publications

References 11 publications

Osteogenic potential of Rosuvastatin immobilized on silk fibroin nanofibers using argon plasma treatment

Osteogenic potential of Rosuvastatin immobilized on silk fibroin nanofibers using argon plasma treatment

Electrospun core-sheath poly(vinyl alcohol)/silk fibroin nanofibers with Rosuvastatin release functionality for enhancing osteogenesis of human adipose-derived stem cells

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