Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions

Abstract

In addition to an initial socket discomfort, ocular prosthesis (OP) installation may allow the adherence of fungi and/or bacteria due to the superficial characteristics of the prosthesis’ material, use of inadequate cleansing solutions and methods, or because the void located between the internal portion of the prosthesis and the anophthalmic cavity (AC) mucosa. Objective: The aim of this study was to evaluate OP biofilm formation and the level of contamination of the internal portion of the OP and the AC in 24 patients. Material and Methods: Material was collected from the AC at the beginning of the study and 15 days after cleansing of the OP with 3 cleansing solutions: a neutral liquid soap, a multiuse solution for contact lens (Complete) and 0.12% chlorhexidine (Periogard). The collected materials were sowed in Petri dishes containing selective media for aerobic and facultative microorganisms, specifically staphylococci (Hipersalt agar with egg yolk), aerobic microorganisms (Brain Heart Infusion Blood Agar), streptococci (Mitis salivarius Agar), gram-negative bacilli (MacConkey Agar) and yeasts (Chromagar CandidaTM), incubated at 35°C or 37°C and the number of colony forming units were counted. Data were analyzed statistically by ANOVA, Friedman’s test and Spearman’s correlation. Results: Aerobic microorganisms, gram-negative bacilli and S. aureus were found in the OP biofilm and in the AC. There was statistically significant difference (p<0.05) between the number of microorganisms before and after the use of the cleansing solutions. Conclusion: There was positive correlation with respect to the microorganisms present in the OP biofilm and AC for the 4 proposed treatments, indicating that the decrease of OP contamination leads to AC contamination as well.

Custom Made Ocular Prosthesis at Optometry Clinic Universiti Kebangsaan Malaysia (UKM)

“…Generally, cleaning with mild antibacterial soap, multipurpose contact lens solution and chlorohexidine disinfectant has been found effective (Paranhos et al 2007). Abrasive disinfectant usage is discouraged as the acrylic material has been found to have physical property changes with disinfectant, which may decrease its durability (Moreno et al 2012).…”

Effect of different disinfectants on the microhardness and roughness of acrylic resins for ocular prosthesis

“…The samples in the NES, OPF, HYC and CHX groups were disinfected daily employing manual friction with gauze for 1 min, followed by rinsing in running water for 30 s 13,17 . The samples in the NES, OPF, HYC and CHX groups were disinfected daily employing manual friction with gauze for 1 min, followed by rinsing in running water for 30 s 13,17 .…”

Influence of different disinfecting solutions on the color change of artificial irises used in ocular prostheses

“…Nevertheless, in average, the ocular prostheses are only considered effective for a period of up to 5 years, and afterward, they need to be remade, mainly due to color instability of the artificial iris. The samples in the NES, OPF, HYC, and CHX groups were disinfected daily employing manual friction with gauze for 1 min, followed by rinsing in running water for 30 s . The samples in the EFF group were cleaned three times a week.…”

“…Nevertheless, in average, the ocular prostheses are only considered effective for a period of up to 5 years, and afterward, they need to be remade, mainly due to color instability of the artificial iris. The samples in the NES, OPF, HYC, and CHX groups were disinfected daily employing manual friction with gauze for 1 min, followed by rinsing in running water for 30 s. 10,31 The samples in the EFF group were cleaned three times a week. Cleaning involved immersing them in a solution of distilled water containing an effervescing tablet at an initial temperature of 378C for 15 min, followed by rinsing in running water for 30 s. 32 When not being disinfected, all of the samples were stored in saline solution at 37 6 28C.…”