Evaluation of nutritional quality and antioxidant potential of pigeonpea genotypes

Abstract

Three released cultivars, forty four advance breeding lines and three wild species of pigeonpea ( L. Millsp) were evaluated for nutritional, antinutritional traits and antioxidant potential so as to identify promising genotypes. The average content of total soluble sugars, starch and total soluble proteins was found to be 43.66, 360.51 and 204.54 mg/g, respectively. Antioxidant potential in terms of free radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), reducing power, hydroxyl radical scavenging activity and superoxide anion radical scavenging activity was estimated. The diversity was observed in genotypes with all the traits. AL 1960, AL 2000, AL 2009 and AL 2046 had high total soluble proteins, medium antinutritional factors and good antioxidant potential. AL 201, AL 1931, AL 1932, AL 1960, AL 2046, AL 2049 and AL 2060 had good nutritional value as protein and starch content ranged from 20 to 23 and 42 to 52%, respectively. Wild species 1CP15683/W15 had lower carbohydrates, proteins, and antinutritional traits while high antioxidant potential due to high total phenols, DPPH, FRAP and reducing power. The diversity observed in genotypes with all the traits could be further used to develop nutritionally important genotypes.

The Effects of Domestication on Secondary Metabolite Composition in Legumes

“…Several wild chickpea accessions have higher seed saponin levels than cultivated chickpea accessions (Kaur et al, 2019). Several wild pigeonpea (Cajanus scarabaeoides) accessions have higher seed saponin contents than the cultivated pigeonpea accessions (Cajanus cajan; Sekhon et al, 2017). However, cultivated pigeonpea accessions do not necessarily have lower seed saponin contents than the wild accessions (Sekhon et al, 2017).…”

“…Several wild pigeonpea (Cajanus scarabaeoides) accessions have higher seed saponin contents than the cultivated pigeonpea accessions (Cajanus cajan; Sekhon et al, 2017). However, cultivated pigeonpea accessions do not necessarily have lower seed saponin contents than the wild accessions (Sekhon et al, 2017). Seed saponin content is not the sole factor leading to the insect resistance of legumes.…”

Comparison of cultivated and wild chickpea genotypes for nutritional quality and antioxidant potential

“…Chickpea seed powder was extracted twice with 15 ml of 80% ethanol and 70% ethanol by keeping the tubes fitted with water condensers in boiling water bath for 20 min. The supernatants after each extraction were collected and pooled and total soluble sugars were estimated by using phenol sulphuric acid method as described previously by Sekhon et al (2017). The collected dried soluble sugar free residue was used to extract and estimate starch using perchloric acid.…”

“…Antinutritional factors such as phytic acid, tannins, trypsin inhibitor and saponins were determined from powdered chickpea seeds by the methods as described previously by Sekhon et al (2017). Phytic acid was extracted with 1.2% HCl and precipitated with ferric chloride and inorganic phosphorus was estimated.…”

“…The pooled supernatants were used to measure antioxidant capacity of chickpea genotypes. Total phenols, flavonols, total reducing power, ferric reducing antioxidant power and free radical scavenging activities such as DPPH (2,2-diphenyl-1-picryl hydrazyl) free radical, hydroxyl radical, superoxide anion radical and nitric oxide radical were estimated in seeds of cultivated and wild species by the methods as described previously by Sekhon et al (2017).…”