Evaluation of nutrient supplementation to charcoal-treated and untreated rice straw hydrolysate for xylitol production by Candida guilliermondii

Mussatto, Solange I.; Roberto, Inês Conceição

doi:10.1590/s1516-89132005000300020

Cited by 22 publications

(6 citation statements)

References 14 publications

(10 reference statements)

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“…In the first phase, we compared two common detoxification procedures—separation by adsorption inhibitors on activated carbon and over-liming. Theoretically, overliming is effective due to precipitation or chemical destabilization of inhibitors [ 13 ] and activated charcoal could improve the fermentability of hydrolysate by absorbing phenolic compounds and other inhibitory substances [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Production of Polyhydroxyalkanoates Using Hydrolyzates of Spruce Sawdust: Comparison of Hydrolyzates Detoxification by Application of Overliming, Active Carbon, and Lignite

et al. 2017

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Polyhydroxyalkanoates (PHAs) are bacterial polyesters which are considered biodegradable alternatives to petrochemical plastics. PHAs have a wide range of potential applications, however, the production cost of this bioplastic is several times higher. A major percentage of the final cost is represented by the price of the carbon source used in the fermentation. Burkholderia cepacia and Burkholderia sacchari are generally considered promising candidates for PHA production from lignocellulosic hydrolyzates. The wood waste biomass has been subjected to hydrolysis. The resulting hydrolyzate contained a sufficient amount of fermentable sugars. Growth experiments indicated a strong inhibition by the wood hydrolyzate. Over-liming and activated carbon as an adsorbent of inhibitors were employed for detoxification. All methods of detoxification had a positive influence on the growth of biomass and PHB production. Furthermore, lignite was identified as a promising alternative sorbent which can be used for detoxification of lignocellulose hydrolyzates. Detoxification using lignite instead of activated carbon had lower inhibitor removal efficiency, but greater positive impact on growth of the bacterial culture and overall PHA productivity. Moreover, lignite is a significantly less expensive adsorbent in comparison with activated charcoal and; moreover, used lignite can be simply utilized as a fuel to, at least partially, cover heat and energetic demands of fermentation, which should improve the economic feasibility of the process.

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Section: Resultsmentioning

confidence: 99%

Production of Polyhydroxyalkanoates Using Hydrolyzates of Spruce Sawdust: Comparison of Hydrolyzates Detoxification by Application of Overliming, Active Carbon, and Lignite

et al. 2017

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“…Growth of Escherichia coli LY01 on xylose, for example, was only affected by syringaldehyde concentrations higher than 2.5 g/l [18], while growth of Candida shehatae and Pichia stipitis was affected only by concentrations of this compound higher than 1.5 g/l [2]. The concentration thresholds used in the present work were chosen based on concentration values found in rice straw hemicellulosic hydrolysate obtained by diluted-acid hydrolysis (1% w/v H 2 SO 4 , 120°C, 30 min), which has been reported as a fermentation medium with great potential for use in xylitol production [12]. Understanding the inhibitory action of the toxic compounds present in this hydrolysate may be of great importance to improve bioconversion results achieved to date.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii

Pereira¹,

Mussatto²,

Roberto³

2010

J Ind Microbiol Biotechnol

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The inhibitory action of acetic acid, ferulic acid, and syringaldehyde on metabolism of Candida guilliermondii yeast during xylose to xylitol bioconversion was evaluated. Assays were performed in buffered and nonbuffered semidefined medium containing xylose as main sugar (80.0 g/l), supplemented or not with acetic acid (0.8-2.6 g/l), ferulic acid (0.2-0.6 g/l), and/or syringaldehyde (0.3-0.8 g/l), according to a 2(3) full factorial design. Since only individual effects of the variables were observed, assays were performed in a next step in semidefined medium containing different concentrations of each toxic compound individually, for better understanding of their maximum concentration that can be present in the fermentation medium without affecting yeast metabolism. It was concluded that acetic acid, ferulic acid, and syringaldehyde are compounds that may affect Candida guilliermondii metabolism (mainly cell growth) during bioconversion of xylose to xylitol. Such results are of interest and reveal that complete removal of toxic compounds from the fermentation medium is not necessary to obtain efficient conversion of xylose to xylitol by Candida guilliermondii. Fermentation in buffered medium was also considered as an alternative to overcome the inhibition caused by these toxic compounds, mainly by acetic acid.

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“…For detoxification of PPWH, charcoal treatment was performed using 2.5% activated charcoal in PPWH while kept on shaking for one hour. Subsequently the supernatant was neutralized with pellets of sodium hydroxide followed by filtration ( Mussatto and Roberto, 2005 , González et al, 2003 ).…”

Section: Methodsmentioning

confidence: 99%

Pomegranate peels waste hydrolyzate optimization by Response Surface Methodology for Bioethanol production

Chaudhary

Hussain

Aihetasham

et al. 2021

Saudi Journal of Biological Sciences

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Unwanted agricultural waste is largely comprised of lignocellulosic substrate which could be transformed into sugars. The production of bioethanol from garbage manifested an agreeable proposal towards waste management as well as energy causation. The goal of this work is to optimize parameters for generation of bioethanol through fermentation by different yeast strains while Saccharomyces cerevisiae used as standard strain. The low cost fermentable sugars from pomegranate peels waste (PPW) were obtained by hydrolysis with HNO 3 (1 to 5%). The optimum levels of hydrolysis time and temperature were elucidated via RSM (CCD) ranging from 30 to 60 min and 50 to 100 °C respectively. The result shows that optimum values (g/L) for reducing sugars was 61.45 ± 0.01 while for total carbohydrates was 236 ± 0.01. These values were found when PPW was hydrolyzed with 3% HNO 3 , at 75 °C for one hour. The hydrolyzates obtained from the dilute HNO 3 pretreated PPW yielded a maximum of 0.43 ± 0.04, 0.41 ± 0.03 g ethanol per g of reducing sugars by both Metchnikowia sp . Y31 and M. cibodasensis Y34 at day 7 of ethanologenic experiment. The current study exhibited that by fermentation of dilute HNO 3 hydrolyzates of PPW could develop copious amount of ethanol by optimized conditions.

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Evaluation of nutrient supplementation to charcoal-treated and untreated rice straw hydrolysate for xylitol production by Candida guilliermondii

Abstract: Xylitol was produced by

Cited by 22 publications

References 14 publications

Production of Polyhydroxyalkanoates Using Hydrolyzates of Spruce Sawdust: Comparison of Hydrolyzates Detoxification by Application of Overliming, Active Carbon, and Lignite

Production of Polyhydroxyalkanoates Using Hydrolyzates of Spruce Sawdust: Comparison of Hydrolyzates Detoxification by Application of Overliming, Active Carbon, and Lignite

Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii

Pomegranate peels waste hydrolyzate optimization by Response Surface Methodology for Bioethanol production

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