Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (<i>Nicotiana tabacum</i>) cv. Coker 139

Dhillon, Sukhraj S.; Wernsman, E. A.; Miksche, Jerome P.

doi:10.1139/g83-029

Cited by 70 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar nuclear DNA content increase in anther-derived dihaploid plants was also detected using cytophotometric analysis and thermal denaturation kinetics in Nicotiana tabacum (Dhillon et al, 1983).…”

Section: Development-induced Dna Content Variationsupporting

confidence: 70%

Developmental and environmental variation in genomes

2009

Heredity

View full text Add to dashboard Cite

The genetic make-up of an organism, established at fertilization, is not conventionally expected to change during development unless mutation occurs. However, there is actually evidence that considerable variation can arise. Some of these changes may occur in response to the environment. This article reviews such variations in genome size or DNA content (excluding ploidy-level changes). The variation can be generated by processes, including high-frequency chromosomal recombination, transposition, cis-element-enhanced gene amplification and repetitivesequence-based changes in nuclear DNA content. Environmentally induced and developmentally regulated genomic variation (ED-genomic variation or ED-genetic variation) can be found in both coding and non-coding sequences, and is often non-Mendelian in its inheritance pattern. Changes can depend on development (for example, propagation method, seed/fruit position on plants, embryo stage, etc.) and occur in response to the environment (for example, light, temperature, herbicide, salinity, fertilizer, land slope direction, pathogen infection, etc.). Some plants have meiotic (or rejuvenation) corrections, which restore their genome sizes to a certain degree. However, Mendelian inheritance and acquired inheritance of the variants occur, and both inheritance types may be different expressions evolved for the same adaptive responses. With this perspective, the terms 'pure-breeding line' or 'stable cultivar' may only be appropriate for a given mode of reproduction or propagation, and for a given environment. ED-genomic variation appears to be an essential component of differentiation, development and adaptation. Consequently, modern molecular biology tools, such as microarray hybridization and new sequencing technology, should be directed towards a more comprehensive evaluation of ED-genomic variation.

show abstract

Section: Development-induced Dna Content Variationsupporting

confidence: 70%

Developmental and environmental variation in genomes

2009

Heredity

View full text Add to dashboard Cite

show abstract

“…Increases in the amount of nuclear DNA per cell were detected in doubled haploid plants of tobacco [6,7]. These plants had the expected 2n chromosome number, but more DNA than expected for diploid cells.…”

Section: Introductionmentioning

confidence: 81%

Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants

Moore

Sink

1988

Plant Mol Biol

View full text Add to dashboard Cite

Restriction fragment polymorphisms were used to identify and quantify the nuclear contributions from each parent to somatic hybrid plants between tomato (Lycopersicon esculentum Mill.) cv. Sub-Arctic Maxi and Solanum lycopersicoides Dun. Three single-copy clones, 2-13, 2-17, and 3-288, and a clone for the 45s ribosomal RNA, pHA2, all mapped to chromosome 2 of tomato, were used in analysis of 47 somatic hybrids. The amount of hybridizing probe for each parental band was quantified by densitometry of the autoradiograph film. Analyses with the three single-copy clones indicated that there were more than two S. lycopersicoides copies in most somatic hybrid plants. For at least one somatic hybrid there was a loss of one tomato copy. No evidence was found for more than two copies donated from tomato or loss of a copy from S. lycopersicoides. Most of the observed variation in copy number of the single-copy clones was consistent with chromosomal changes occurring in the suspension cells from which S. lycopersicoides parental protoplasts were derived.The number of copies of rDNA derived from each parent varied independently of the number of copies of single-copy clones from each parent. Changes in the copy number of rDNA occurred in both tomato and S. lycopersicoides genomes.

show abstract

“…There have been no convincing demonstrations of the type of mutation process that might be occurring in the microspore or pollen grain, or in their immediate products. Various suggestions have been made concerning possible qualitative or quantitative differences between the DNA of the vegetative cell of the pollen grain from which, at least in tobacco, the pollen plant is produced, and the DNA of the egg cell from which the normal zygote is derived (De Paepe eta!.,1981Brown and Wernsman, 1982;Dhillon et a!., 1983). However, such suggestions remain as speculation.…”

Section: Discussior'imentioning

confidence: 99%

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

et al. 1984

View full text Add to dashboard Cite

SUMMARYSeventy-four spontaneously doubled microspore-derived (M) lines of Hordeum vulgare cv. Sabarlis were grown in the field and their agronomic performance compared with that of 16 selfed seed-derived (S) lines. It was found that two of the characters scored, namely mildew susceptibility, and growth habit did not show any differences between S and M lines. In contrast, the other seven characters, days to awn emergence, plant height, neck length, number of fertile tillers, number of grains on the main tiller, grain yield on the main tiller, and thousand grain weight, all showed significant differences between the M lines and not between the S lines. Overall, the M populations tended to be later to awn emergence, and to be taller with longer necks and fewer tillers than the S population.Possible explanations for this variation are discussed in relation to other examples of "somaclonal variation" described amongst plants regenerated from cultured cells.

show abstract

Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum) cv. Coker 139

Cited by 70 publications

References 0 publications

Developmental and environmental variation in genomes

Developmental and environmental variation in genomes

Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

Contact Info

Product

Resources

About