Evaluation of Novel Mouse-Specific Germ Cell Gene Expression in Embryonic Stem Cell-Derived Germ Cell-Like Cells<i>In Vitro</i>with Retinoic Acid Treatment

Tabari, Maryam Gholamitabar; Jorsaraei, Seyed Gholam Ali; Ghasemzadeh-Hasankolaei, Mohammad; Ahmadi, Ali Asghar; Amirikia, Mehdi

doi:10.1089/cell.2017.0057

Cited by 3 publications

(8 citation statements)

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“…They revealed differentiation of the cell lines by forming EBs via coexpression of Oct4 and Mvh for identifying the novel PGCs in vitro (Eguizabal et al, ). Gholamitabar Tabari et al () assessed the effects of retinoic acid on expressing the gene of mice‐specific germ cells in ESCs‐derived germ cells in vitro. They showed the increased expression level of Mvh in ESCs given treatment with RA in comparison to the ones of without RA (Gholamitabar Tabari et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Gholamitabar Tabari et al () assessed the effects of retinoic acid on expressing the gene of mice‐specific germ cells in ESCs‐derived germ cells in vitro. They showed the increased expression level of Mvh in ESCs given treatment with RA in comparison to the ones of without RA (Gholamitabar Tabari et al, ). Thus, this study finding had consistency with the present research outputs.…”

Section: Discussionmentioning

confidence: 99%

“…no. R2625‐50MG; Eguizabal, Shovlin, Shovlin, Durcova‐Hills, Surani, & McLaren, ; Ghasemzadeh‐Hasankolaei, Eslaminejad, Batavani, & Sedighi‐Gilani, ; Gholamitabar Tabari et al, ; Huang et al, ; Li et al, ; Xuemei et al, ) at days 0, 4 and 7. miPSCs‐derived EBs that had not treated with RA were used as a negative control (Figure ). The protocol of differentiating miPSCs into PGCs in vitro via inducing RA has been performed based on the previous studies (Eguizabal et al, ; Li et al, ; Yang et al, ; Zhu et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Diagnosing miPSCs differentiation into germ cells has been done mainly via analysing germ cell‐specific gene and expressing protein in complicated stem cells cultures. Several research showed expressing genes such as Ddx4 (Mvh) , Stra8 , AKAP3 and Sycp3 , which enhanced progressing nascent PGCs to begin meiosis and generate haploid cells specific to germ cell growth (Gholamitabar Tabari, Jorsaraei, Jorsaraei, Ghasemzadeh‐Hasankolaei, Ahmadi, & Amirikia, ; Li et al, ; Silva et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

et al. 2019

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This research aimed to explore the impacts of retinoic acid (RA)/17β-estradiol (E) induction and embryoid body formation to enhance differentiation of mouse-induced pluripotent stem cells (miPSCs) into male germ cells in vitro. Flow cytometry and qPCR were conducted to describe miPSCs differentiation process. Various temporal expression profiles of germ cell-related genes were traced. Stra8 gene expression increased in the RA group on the 4th day compared to other groups. The RA group experienced a more significant increase than E group. The expression of Sycp3 increased in RA + E group on 4th day compared with other groups. Expression of AKAP3 enhanced in the RA + E group than other groups on day 4. Moreover, miPSCs showed that this gene expression in the RA + E group was increased in comparison to RA and E groups on day 7. AKAP3 gene expression on day 7 of miPSCs decreased in RA and E groups. Flow cytometry data indicated that 3%-8% of the cells in sub-G1 stage were haploid after RA and E induction compared to other groups on day 4. This study showed that miPSCs possess the power for differentiating into male germ cells in vitro via formation of embryoid body by RA with/or E induction.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

et al. 2019

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“…no. R2625‐50MG) at days 0, 4, and 7. The items that were not treated with RA served as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Retinoic acid and/or progesterone differentiate mouse induced pluripotent stem cells into male germ cells in vitro

Mahabadi

Tameh

Talaei

et al. 2019

J of Cellular Biochemistry

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Numerous reagents were employed for differentiating induced pluripotent stem cells (iPSCs) into male germ cells; however, the induction procedure was ineffective. The aim of this study was to improve the in vitro differentiation of mice iPSCs (miPSCs) into male germ cells with retinoic acid (RA) and progesterone (P). miPSCs were differentiated to embryoid bodies (EBs) in suspension with RA with or without progesterone for 0, 4, and 7 days. Then, the expression of certain genes at different stages of male germ cell development including Ddx4 (pre meiosis), Stra8 (meiosis), AKAP3 (post meiosis), and Mvh protein was examined in RNA and/or protein levels by real-time polymerase chain reaction or flow cytometry, respectively. The Stra8 gene expression increased in the RA groups on all days. But, expression of this gene declined in RA + P groups. In addition, an increased expression of Ddx4 gene was observed on day 0 in the P group. Also, a significant upregulation was observed in the expression of AKAP3 gene in the RA + P group on days 0 and 4. However, gene expression decreased in P and RA groups on day 7. The expression of Mvh protein significantly increased in the RA group on day 7. The Mvh expression was also enhanced in the P group on day 4, but it decreased on day 7, while this protein upregulated on day 0 and 7 in the RA + P group. The miPSCs have the capacity for in vitro differentiation into male germ cells by RA and/or progesterone. However, the effects of these inducers depend on the type of combination and an effective time. K E Y W O R D Sdifferentiation, induced pluripotent stem cells, male germ cells, progesterone, retinoic acid | INTRODUCTIONMany couples with the infertility problem encounter environmental and genetic factors. 1,2 Today, assisted reproductive technology helps patients to treat their infertility problems that otherwise wasn't possible with common cures. However, patients with abnormal eggs or azoosperima and mutation in germ cells cannot take advantage of assisted reproductive technology. 3,4 According to the research, under certain conditions the stem cells can differentiate into germs cells that opens an avenue for treating infertility. [5][6][7] Stem cells, as undifferentiated cells, exist in all stages of life, i.e. embryonic, fetal, and adult stages, and create differentiated cells that in turn How to cite this article: Mahabadi JA, Tameh AA, Talaei SA, et al. Retinoic acid and/or progesterone differentiate mouse induced pluripotent stem cells into male germ cells in vitro.

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RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Yan

Liu

et al. 2019

Journal Cellular Physiology

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Previous studies have shown that primordial germ cell‐like cells (PGCLCs) can be obtained from human, porcine and mouse skin‐derived stem cells (SDSCs). In this paper, we found retinoic acid (RA), the active derivative of vitamin A, accelerated the growth of porcine primordial germ cells (pPGCs) and porcine PGCLCs (pPGCLCs) which were derived from porcine SDSCs (pSDSCs). Moreover, flow cytometry results revealed that the proliferation promoting effect of RA was attenuated by U0126, a specific inhibitor of extracellular signal‐regulated kinase (ERK). Western blot analysis showed the protein level of ERK, phosphorylated ERK, cyclin D1 (CCND1), and cyclin‐dependent kinase 2 (CDK2) increased after stimulation with RA, and this effect could also be abolished by U0126. Our data revealed that ablation of ERK expression by U0126 should significantly decrease proliferation of pPGCLCS. This reduction was because CCND1 and CDK2 proteins level decrease and subsequently the pPGCLCs were arrested in the G0/G1 phase. In addition, we also confirmed RA indeed promoted the proliferation of pPGCs isolated from porcine fetal genital ridges in vitro. Furthermore, our data indicated that DNA methylation pattern were changed in pPGCLCs and this pattern were more similar to pPGCs.

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Evaluation of Novel Mouse-Specific Germ Cell Gene Expression in Embryonic Stem Cell-Derived Germ Cell-Like CellsIn Vitrowith Retinoic Acid Treatment

Cited by 3 publications

References 41 publications

Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

Retinoic acid and/or progesterone differentiate mouse induced pluripotent stem cells into male germ cells in vitro

RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

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