Evaluation of non-synonym mutation in DGAT1 K232A as a marker for milk production traits in Ongole cattle and Murrah buffalo from Southern India

Sudhakar, K.; Thiruvenkadan, A.K.; Muthialu, Nagarajan; Saravanan, R.; Vinoo, R.; Metta, Muralidhar

doi:10.1007/s11250-021-02560-2

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…The high proportion of the lysine variant K232 observed in the current study might contribute to the higher fat content in native cattle in Ethiopia and other Bos indicus breeds than in HF cross cattle. The frequency of the lysine variant K232 in the investigated Boran * HF cross cattle is similar to that found in studies of other HF populations or their crosses, which have reported a range from 0.21 to 0.63 (Ahani et al, 2015;Akyüz et al, 2015;Komisarek and Kolenda, 2016;Bhat et al, 2017;Li et al, 2020;Krovvidi et al, 2021). The differences in the frequency of the lysine variant K232 among breeds might result from different breeding goals and the considerable Holstein contribution to the blood level of Boran * HF cross cattle.…”

Section: Allele and Genotype Frequencies Of The K232a Protein Variantsupporting

confidence: 85%

“…The finding in the present study indicated the tendency of fixation of DGAT1 K allele in Horro cattle did not support its use as a reliable universal marker for milk yield and composition traits for the population. Fixation of the DGAT1 K allele in ongole and buffalo breeds in India was noted by Krovvidi et al (2021), whereas Kaupe et al (2004) found fixation of the DGAT1 A allele in five Bos taurus breeds (Belgian Blue beef, Gelbvieh, Hereford, Pinzgaurer, and Slavonian Syrmian). The high proportion of the lysine variant K232 observed in the current study might contribute to the higher fat content in native cattle in Ethiopia and other Bos indicus breeds than in HF cross cattle.…”

Section: Allele and Genotype Frequencies Of The K232a Protein Variantmentioning

confidence: 99%

“…Grisart et al (2004) demonstrated that the lysine variant, which represents the "wild type" and is designated the K allele, is distinguished by a higher maximum rate of reaction of the enzyme involved in triglycerides synthesis than the alanine variant, thereby increasing the fat percentage in the animal's milk. The lysine variant has been linked to higher fat yield and fat and protein percentages, whereas the alanine variant has been linked to higher milk and protein yields (Grisart et al, 2002;Krovvidi et al, 2021;Elzaki et al, 2022;Gothwal et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The distribution of the allele frequencies of the DGAT1 K232A mutation has been studied in various Holstein populations and other exotic breeds, and the effects of DAGT1 polymorphisms on milk yield traits have been researched (Bovenhuis et al, 2016;Krovvidi et al, 2021;Li et al, 2021;Gothwal et al, 2022). Information on the effect of DGAT1 K232A on the daily milk yield and milk composition of different cattle breeds is scarce in tropical zebu and their crossbred population.…”

Section: Introductionmentioning

confidence: 99%

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Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

2023

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Non-conservative K232A substitution in the diacylglycerol acyl-CoA acyltransferase 1 (DGAT1) gene has been reported to explain variation in milk traits in cattle. The objective of this study was to estimate allele and genotype frequencies and to assess associations between K232A variants and milk yield and composition in cattle populations of Ethiopia. Blood samples for genomic DNA extraction and milk samples for analysis of milk components were collected from 92 randomly selected cattle of five Ethiopian breeds. Gene-specific primers were used to amplify 278 bp of the exon 8 region of DGAT1. Allele and genotype frequencies were calculated using Power Marker, and the GLM function of SAS software was used to assess the associations of detected genetic variation with milk traits. Boran * Holstein Friesian (HF) crosses produced a higher daily milk yield than the other breeds (p< 0.05), whereas the Boran and Begait breeds produced milk with higher fat and protein contents (p< 0.05), and Horro produced milk with a higher content of lactose, than the other breeds studied (p< 0.05). Alleles K and A and genotypes AA, KA, and KK were detected. The frequency of K232 ranged from 0.50 in Boran * HF crosses to 0.97 in the Horro population. The frequency of the KK and KA genotypes in the zebu population ranged from 0.50 to 0.94 and from 0.03 to 0.50, respectively. The AA genotype was associated with higher milk yield in Boran * HF crosses, whereas the KA genotype was associated with higher milk yield in the zebu populations (p< 0.05). The fat and lactose contents of milk produced with the KA genotype were lower than those of milk produced with the KK genotype in all genetic groups (p< 0.05). Substitution of one copy of the K allele led to a significant (p< 0.05) increase in fat content, of up to 0.81%, a decrease in daily milk yield of up to 3 L, and a decrease in lactose content of 0.58% in the sampled populations. The association study confirmed that the DGAT1 K232A marker had significant effects on daily milk yield, milk fat and lactose contents in the investigated cattle. These results suggested that the DGAT1 K232A marker may be utilized to accelerate future molecular breeding of dairy cattle, tropical zebu, their crossbreeds, after validation in a larger population.

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Section: Allele and Genotype Frequencies Of The K232a Protein Variantsupporting

confidence: 85%

Section: Allele and Genotype Frequencies Of The K232a Protein Variantmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

2023

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“…Functional and correlation analyses have identified FATP1 [43], LXRα [44], PPARγ [45], ELOVL6 [46], HSD17B4 [47], BTN1A1, XDH, PLIN1, PLIN2 [48,49], ABCD1 [50] and INSIG2 [51] as having regulatory roles in milk fat synthesis and lactation. In particular, DGAT1 [52,53], FASN [54] and PPARGC1A [55] influenced milk production, as did Casein [56] and OAT [57]. Omics-based studies have also been used to investigate milk production.…”

Section: Advances In Molecular Breeding Of China Dairy Buffalomentioning

confidence: 99%

Genetic Bases and Molecular Breeding of Key Economic Traits in China Dairy Cattle: A Progress Report

Ma¹,

Li²,

Xue³

et al. 2022

IJCCR

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Animal growth is a coordinated developmental process that requires altering the expression of hundreds to thousands of genes to modify many biochemical and biological signaling cascades. The genetic analysis of economic traits and molecular breeding research are hotspots for animal husbandry and, in the case of Chinese dairy cattle, much progress has been made in recent years. Actually, the level of information of molecular events at the transcriptional, biochemical, hormonal, and metabolite levels underlying animal development process has increased considerably. The current review summarizes progress in research into the genetic basis of economic traits and molecular breeding of dairy cows, dual-purpose cattle and dairy buffalo, to better understand the molecular switch involved in development process with important consequences from a breeding point of view.

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Polymorphism of the Leptin and Diacylglycerol-O-Acyltransferase Genes in Holsteinized Black and White Bulls

Lamara¹,

Zagidullin

Ahmetov³

et al. 2022

Agrobiotechnologies and Digital Farming

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Allelic polymorphism was studied and the frequency of occurrence of genotypes for leptin (LEP) and diacylglycerol-O-acyltransferase (DGAT1) genes in Black-and-White bulls was determined, their belonging to different genealogical lines of Holstein cattle. Among the animals of the analyzed sample, there was the highest frequency of genotypes LEP CT (53.4 %), DGAT1 AA (50.0 %) and alleles LEP C (0.61), DGAT1 A (0.72). The studied sample of bull breeds by identification to the Holstein breed was structurally represented by the genealogical lines of V. Aidial (77.6%), M. Chiftein (8.6%), R. Sovering and S.T. Rokit (6.9% each). Similar studies of these bulls belonging to different genealogical lines showed that the highest occurrence of genotypes and alleles was LEP CT (57.8%), DGAT1 AA, DGAT1 AK (46.7% each), LEP C (0.60), DGAT1 A (0.70) on the line of V. Aidial, LEP CC, LEP CT (by 50.0%), DGAT1 AA (100.0%), LEP C (0.75), DGAT1 A (1.0) by R. Sovering line, LEP CC, LEP CT (50.0% each), DGAT1 AA (75.0%), LEP C (0.75), DGAT1 A (0.88) by S.T. Rokit, LEP CC, LEP TT (40.0% each), DGAT1 AK (60.0%) on the line of M. Chieftain, respectively. However, it should be noted that according to the M. Chieftain line, the frequency of occurrence of alleles LEP C, LEP T and DGAT1 A, DGAT1 K for the two studied genes was equal and amounted to 0.50. It should also be noted that in the population of bulls with different genotypes for the LEP, DGAT1 genes and unequal linear affiliation to the Holstein breed, the genetic balance is not disturbed.

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Evaluation of non-synonym mutation in DGAT1 K232A as a marker for milk production traits in Ongole cattle and Murrah buffalo from Southern India

Cited by 4 publications

References 26 publications

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia

Genetic Bases and Molecular Breeding of Key Economic Traits in China Dairy Cattle: A Progress Report

Polymorphism of the Leptin and Diacylglycerol-O-Acyltransferase Genes in Holsteinized Black and White Bulls

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