Evaluation of nitrogen source, concentration and feeding mode for co-production of fucoxanthin and fatty acids in Phaeodactylum tricornutum

Wu, Zhengshuai; Qiu, Shuang; Abbew, Abdul-Wahab; Chen, Zhipeng; Liu, Yanlin; Zuo, Jinyu; Ge, Shijian

doi:10.1016/j.algal.2022.102655

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…Different nitrogen source including ammonia, nitrate, and urea, have been used for microalgal growth, as presented in Table 1. In large-scale microalgal cultivation, urea often holds a significant position among them because to its plentiful supplies, simple preparation, and inexpensive price [33]. Nonetheless, the results obtained in [23,33] show that among the three different nitrogen sources (NaNO3, (NH4)2CO3, and NH4Cl), sodium nitrate (NaNO3) is the best option, as both other nitrogen sources contain ammonium, which at higher concentrations (above 0.05 g/L) can drop the pH of the medium to nearly 1 [23].…”

Section: Media Compositionmentioning

confidence: 99%

“…In large-scale microalgal cultivation, urea often holds a significant position among them because to its plentiful supplies, simple preparation, and inexpensive price [33]. Nonetheless, the results obtained in [23,33] show that among the three different nitrogen sources (NaNO3, (NH4)2CO3, and NH4Cl), sodium nitrate (NaNO3) is the best option, as both other nitrogen sources contain ammonium, which at higher concentrations (above 0.05 g/L) can drop the pH of the medium to nearly 1 [23]. Chu No.10 medium, Blue-Green 11 medium (BG11), and Bristol and Bold's basal medium (BBM) were proved to be excellent media for microalgae cultivation.…”

Section: Media Compositionmentioning

confidence: 99%

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Microalgae cultivation: from CO₂ fixation to single-cell protein production

Ibrahim Mze,

Azmi,

Mohd Puad

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Bio-sequestration of CO2 using microalgae to recycle CO2 into valuable products such as single-cell proteins (SCP) is one of the most promising fields nowadays. Microalgae are able to use CO2 as their carbon source and subsequently build carbohydrates, proteins, nucleic acids, and lipids. Nevertheless, all microalgae strains do not have the same CO2 tolerance and culture conditions. Moreover, pure CO2 is less soluble in water, which leads to a low carbon capture and fixation rate. Thus, to optimise SCP production in relation to CO2 mitigation, studies of the enhancement of solubilization of CO2 in water as well as the determination of the optimum process parameter for CO2 fixation and SCP production need to be done. Consequently, this study aims to review the cultivation conditions for single-cell protein production.

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Section: Media Compositionmentioning

confidence: 99%

Section: Media Compositionmentioning

confidence: 99%

Microalgae cultivation: from CO₂ fixation to single-cell protein production

Ibrahim Mze,

Azmi,

Mohd Puad

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Many findings have revealed that different nitrogen concentrations can extensively alter the growth, biochemical composition, and synthesis of bioactive compounds, such as fatty acids and pigments [22,23]. In particular, the influence of nitrogen availability on fatty acids and FX, the two most abundant high-value compounds found in diatoms, has been studied extensively [24,25]. Previous studies have indicated that nitrogen limitation promotes lipid accumulation and reduces FX production.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation

Truong,

Park,

Winarto

et al. 2024

Marine Drugs

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This study aimed to investigate the regulation of fucoxanthin (FX) biosynthesis under various nitrogen conditions to optimize FX productivity in Phaeodactylum tricornutum. Apart from light, nitrogen availability significantly affects the FX production of microalgae; however, the underlying mechanism remains unclear. In batch culture, P. tricornutum was cultivated with normal (NN, 0.882 mM sodium nitrate), limited (LN, 0.22 mM), and high (HN, 8.82 mM) initial nitrogen concentrations in f/2 medium. Microalgal growth and photosynthetic pigment production were examined, and day 5 samples were subjected to fucoxanthin–chlorophyll a/c-binding protein (FCP) proteomic and transcriptomic analyses. The result demonstrated that HN promoted FX productivity by extending the exponential growth phase for higher biomass and FX accumulation stage (P1), showing a continuous increase in FX accumulation on day 6. Augmented FX biosynthesis via the upregulation of carotenogenesis could be primarily attributed to enhanced FCP formation in the thylakoid membrane. Key proteins, such as LHC3/4, LHCF8, LHCF5, and LHCF10, and key genes, such as PtPSY, PtPDS, and PtVDE, were upregulated under nitrogen repletion. Finally, the combination of low light and HN prolonged the P1 stage to day 10, resulting in maximal FX productivity to 9.82 ± 0.56 mg/L/day, demonstrating an effective strategy for enhancing FX production in microalgae cultivation.

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