Evaluation of new Cu(II) complexes as a novel class of inhibitors against plant carbonic anhydrase, glutathione reductase, and photosynthetic activity in photosystem II

Abstract

Increasing inefficiency of production of important agricultural plants raises one of the biggest problems in the modern world. Herbicide application is still the best method of weed management. Traditional herbicides blocking only one of the plant metabolic pathways is ineffective due to the rapid growth of herbicide-resistant weeds. The synthesis of novel compounds effectively suppressing several metabolic processes, and therefore achieving the synergism effect would serve as the alternative approach to weed problem. For this reason, recently, we synthesized a series of nine novel Cu(II) complexes and four ligands, characterized them with different analyses techniques, and carried out their primary evaluation as inhibitors of photosynthetic electron transfer in spinach thylakoids (design, synthesis, and evaluation of a series of Cu(II) based metal-organic complexes as possible inhibitors of photosynthesis, J Photochem Photobiol B, submitted). Here, we evaluated in vitro inhibitory potency of these agents against: photochemistry and carbonic anhydrase activity of photosystem II (PSII); α-carbonic anhydrase from bovine erythrocytes; as well as glutathione reductase from chloroplast and baker's yeast. Our results show that all Cu(II) complexes excellently inhibit glutathione reductase and PSII carbonic anhydrase activity. Some of them also decently inhibit PSII photosynthetic activity.

Is bicarbonate directly used as substrate to participate in photosynthetic oxygen evolution

“…The data is represented by mean ± SE (n = 3 * 4). P, the algal proliferation multiple; f B , the proportion of bicarbonate added as carbon sources to the whole carbon sources used by microalgae; f b , the proportion of bicarbonate utilization pathway to the whole carbon utilization pathway of microalgae; *The initial concentration of NaHCO 3 added in medium; §The data in brackets were calculated values, whereas those out of brackets were corrected values considering the errors from the determination of d 13 C and the difference induced by growth status (Rodionova et al 2017).…”

Fast enzymatic HCO3- dehydration supports photosynthetic water oxidation in Photosystem II from pea

“…(i) there is an absence of relationship between CA and photosynthetic activities in PSII (McConnell et al, 2007); (ii) there is contamination by water-soluble CAs from the stroma and/or from the cytoplasm (Bricker and Frankel, 2011); and (iii) a very low CA activity is observed in PSII (Bricker and Frankel, 2011). However, later in several papers Karacan et al, 2016;Rodionova et al, 2017;, we have shown clear relationship between CA activity, O 2 evolution and photosynthetic electron transfer in PSII. In view of the earlier concerns (Bricker and Frankel, 2011;McConnell et al, 2007), we first checked the possibility of contamination of PSII (isolated by the procedure used in , which is usually the one we use in our research) by watersoluble and membrane bound CAs.…”

“…We thank the late Professor V.V. Klimov (who initiated Barber, G. Samuelsson and G. Govindjee : Vyacheslav (Slava) Klimov (1945Klimov ( -2017: A scientist par excellence, a great human being, a friend, and a Renaissance man. Photosynth.…”

Biological Properties of Transition Metal Complexes with Metformin and Its Analogues

“…Interestingly, several Cu(II) complexes were investigated as potential herbicides for effective weed management [165,166]. The assumption was based on the ability of redoxactive Cu(II) complexes to decrease GSH/GSSG ratio leading to the inhibition of protein synthesis and suppression of cell division [62,167].…”