Evaluation of Nephroprotective Effects of Hydroalcoholic Extract of Cyperus scariosus Linn. in Gentamicin-induced Acute Kidney Injury in Wistar Albino Rats

Gajjar, Khushboo K.; Aiwale, Amol S.; Anovadiya, Ashish; Mevada, Ashvin V.; Baxi, Seema; Tripathi, C. B.

doi:10.17795/jjnpp-34452

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“…In the current study, gentamicin (100 mg/kg/day) administration in albino mice displayed toxicity symptoms, as indicated by increase in serum levels of creatinine, urea, BUN, and uric acid, as well as changes in kidney cellular structure (i.e., tubular atrophy, thinning, and edema in renal parenchyma). In a study conducted by Khushboo K. et al, gentamicin 100 mg/kg/day was also shown to induce nephrotoxicity with similar symptoms [21]. Gentamicin-induced toxic effects were observed to be partially reversed in animals treated with hydroalcoholic extract of Cucumis melo seeds.…”

Section: Discussionmentioning

confidence: 99%

HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of Cucumis melo Seeds in Gentamicin-Induced Renal Damage

et al. 2019

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Background and objectives: Cucumis melo, of family Cucurbitaceae, has traditionally been used to treat variety of kidney disorders. However to best of our knowledge there is no scientific study available that validates its renaoprotective uses. Therefore, this study aimed to evaluate nephroprotective effects of hydroalcoholic extract of Cucumis melo seeds (CMHE) and to identify its phytoconstituents. Materials and Methods: HPLC was performed to identify key phytochemicals of CMHE. Gentamicin (100 mg/kg/day, i.p) was administered to induce nephrotoxicity in Swiss albino mice for 8 days. Gentamicin (100 mg/kg/day, i.p) and oral CMHE were co-administered to mice at doses of 250 and 500 mg/kg to evaluate protective effects of CMHE. Normal control group mice were administered normal saline. Changes in body weights, biochemical and histopathological studies were conducted to establish nephroprotective effects of CMHE. Results: HPLC analysis indicated presence of quercetin, m-coumaric acid, gallic acid, chlorogenic acid, and trans-4-hydroxy-3-methoxy cinnamic acid in CMHE. Mice treated with CMHE showed significant increase in body weight and decrease in kidney weight as compared with toxic control group. Dose-dependent significant decrease in total blood urea nitrogen, serum creatinine, serum urea, and uric acid levels were observed in CMHE-treated groups as compared with toxic control group. Histopathological analysis of CMHE-treated groups showed improvement in kidney structures as compared with toxic control group. Conclusions: Biochemical, histopathological, and phytochemical screening of hydroalcoholic extract of Cucumis melo seeds suggest that it has nephroprotective potential. Furthermore, standardization of extract against identified phytochemicals, as well as long-term toxicological studies are suggested before commencement of clinical trials.

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Section: Discussionmentioning

confidence: 99%