Evaluation of nasal culturing procedures and immunization as applied to the control of Bordetella bronchiseptica rhinitis in swine

Farrington, Daniel O.

doi:10.31274/rtd-180813-4449

Cited by 4 publications

(3 citation statements)

References 57 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Primary swab isolates are usually plated onto selective media containing penicillin, streptomycin, and nystatin (a fungus inhibitor) (56). Other types of selective media have also been used successfully (41,56,57).…”

Section: Downloaded Frommentioning

confidence: 99%

Biology of Bordetella bronchiseptica.

Goodnow¹

1980

Microbiological Reviews

278

166

View full text Add to dashboard Cite

Section: Downloaded Frommentioning

confidence: 99%

Biology of Bordetella bronchiseptica.

Goodnow¹

1980

Microbiological Reviews

278

166

View full text Add to dashboard Cite

“…Although various selective media have been proposed for use by different investigators, none has been proven to be entirely satisfactory. In some cases, specimens plated on selective media that did not yield B. bronchiseptica were not evaluated by another method (5,7,13). This is an important point, since it has been reported that selective media can retard the growth of B. bronchiseptica when the organism is present in low numbers (20).…”

mentioning

confidence: 99%

Nonradioactive colony lift-hybridization assay for detection of Bordetella bronchiseptica infection in swine

Ackermann

Dyer

1995

J Clin Microbiol

View full text Add to dashboard Cite

Current methods for the isolation and identification of Bordetella bronchiseptica from clinical samples are time-consuming and are based, in part, on subjective observations. We describe the use of a Bordetella-specific DNA probe in a nonradioactive colony lift-hybridization assay for the identification of B. bronchiseptica. Eleven of 82 clinical specimens were found to contain B. bronchiseptica by this method, while only 5 of these were reported to contain the organism when the specimens were analyzed by traditional methods. The chromosomal fragment containing a sequence complementary to the probe appeared to be conserved in B. bronchiseptica isolates from swine from a variety of sources. The assay is more rapid than culture and biochemical testing since it can be performed directly on primary culture plates, even when they are heavily contaminated with other bacterial species. Only minimal training is required to accomplish the assay successfully, and the results are easy to interpret.

show abstract

“…Surprisingly, there are only a few reports on the utilization of selective media for the isolation of these microorganisms. Since the work of Farrington and Switzer (12), MacConkey furaltadone medium is commonly used for the recovery of B. bronchiseptica. Isolation of P. multocida in piglets has been done mainly by passage in mice (19).…”

mentioning

confidence: 99%

Comparison of isolation methods for the recovery of Bordetella bronchiseptica and Pasteurella multocida from the nasal cavities of piglets

et al. 1993

View full text Add to dashboard Cite

Nasal swabs from 241 piglets from 12 herds with clinical atrophic rhinitis and 283 piglets from 14 herds without clinical atrophic rhinitis were examined for the presence ofBordeteUla bronchiseptica and/or Pasteurella multocida. For B. bronchiseptica, swabs were streaked on three selective media. Blood agar supplemented with cephalexin was the most satisfactory selective culture medium for the isolation of B. bronchiseptica. For P. multocida, swabs were also streaked on three selective media. Mice were also used for isolation ofP. multocida from the nasal cavities of pigs. The mouse inoculation test was not found to be the definitive test for the isolation of P. mulocida. A significant number of P. mutocida strains were avirulent in the mouse model. The modified Knight medium (without potassium tellurite) was the best single method for isolating P. multocida. However, a combination of mouse passage and direct culture on selective media increased the rate of isolation. There was no marked difference in the prevalence of B. bronchiseptica or P. multocida in swine herds with or without clinical atrophic rhinitis. Both capsular types A and D were present in the nasal cavities of the pigs with or without clinical atrophic rhinitis.

show abstract

Evaluation of nasal culturing procedures and immunization as applied to the control of Bordetella bronchiseptica rhinitis in swine

Cited by 4 publications

References 57 publications

Biology of Bordetella bronchiseptica.

Biology of Bordetella bronchiseptica.

Nonradioactive colony lift-hybridization assay for detection of Bordetella bronchiseptica infection in swine

Comparison of isolation methods for the recovery of Bordetella bronchiseptica and Pasteurella multocida from the nasal cavities of piglets

Contact Info

Product

Resources

About