Evaluation of NaOH treatment of human dura mater implants to obviate Creutzfeldt- Jakob Disease transmission

Clinical effectiveness of processed and unprocessed bone

“…Two treatments known to inactivate prions are the uses of high concentrations of sodium hydroxide or sodium hypochlorite. For soft tissues, there are concerns about the effects of these treatments on the collagenous matrix (Kearney & Johnson, 1991). However, they may be suitable for bone where the collagen is somewhat protected by mineral.…”

Revision Myringoplasty with Solvent‐Dehydrated Human Dura Mater (Tutoplast)

“…The first case of iatrogenic Creutzfeldt-Jakob disease who received cadaveric dura was reported in 1987 14 . Treatment of dura with 1 N sodium hydroxide for 1 hour removes the prions 15 . A new procedure to inactivate the Creutzfeldt-Jakob disease agent was adopted in 1987, and the solvent-processed graft has appeared on the market since then.…”

“…14 Treatment of dura with 1 N sodium hydroxide for 1 hour removes the prions. 15 A new procedure to inactivate the Creutzfeldt-Jakob disease agent was adopted in 1987, and the solventprocessed graft has appeared on the market since then. Transmission of disease after solvent-processed dura graft has not been reported so far, nor did we see any diseases in the follow-up period in our cases.…”

Yorkshire Regional Tissue Bank—Circa 50 Years of Tissue Banking

“…Sixteen years on, yet another new disease, vCJD, has arisen to challenge tissue bankers in the UK and Europe. Rigorous donor testing and validation of sterilisation techniques were early priorities (Kearney 1987;Kearney 1988;Aguirregoicoa and Kearney 1989;Kearney 1989;Kearney and Johnson 1991;Kearney et al 1993;Kearney 1997;. Although the basic philosophy for banking of tissues remained Weerasena et al 1992;Ketheesan et al 1994;Harris et al 1995;Kearney and Lomas 1997;Kearney 1999;Lomas et al 2000;Lomas et al 1999;Fisher et al 1990;Butterfield et al 1990;, developments in cryopreservation of tissues Ingham et al 1993;Ketheesan et al 1997;) and the new field of tissue engineering, as applied to skin cell culture (Rowling et al 1988;Taylor et al 1990;Rowling et al 1990;Walters et al 1995;Khammo et al 1997;Daniels et al 1997;Ingham et al 1998), and blood vessels (Haynes et al 1990, Haynes and, were explored within the continuing R&D programme.…”