Evaluation of Mycobacterium tuberculosis Early Secreted Antigenic Target 6 Recombinant Protein as a Diagnostic Marker in Skin Test

Moradi, Jale; Mosavari, Nader; Ebrahimi, Mahmoud; Arefpajohi, Reza; Tebianian, Majid

doi:10.1016/j.phrp.2014.12.002

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…A study in Iran also showed that the recombinant early secretory antigenic target 6 is more specific marker to detect of Mycobacterium tuberculosis infection than Tuberculin skin test [18]. In our study 38 cases (32%) did not have a BCG scar and PPD test was negative in these cases.…”

Section: Discussionsupporting

confidence: 45%

A New Formula for BCG Scar and Tuberculin Test Response

Ghaderi¹

2017

MOJI

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Section: Discussionsupporting

confidence: 45%

A New Formula for BCG Scar and Tuberculin Test Response

Ghaderi¹

2017

MOJI

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“…Importantly, others and we have demonstrated that the MM thickness is around 7–8 nm 6 , 8 despite the presence of the very long chain of MA (up to C90), which raises important questions about the exact native conformation of MA. This outer membrane is also supposed to contain porins for the uptake of small hydrophilic molecules – porins characterized only in the case of M. smegmatis ( Msm ) 16 and membrane machineries involved in the secretion of virulence factors such as the well described ESAT-6 and Cfp10 proteins 17 – 19 , whose mechanism of translocation across the MM remains largely unknown. In contrast to the OL and MM, the PM has been well studied and proved to be similar of those to other microorganisms 3 .…”

Section: Introductionmentioning

confidence: 99%

Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane

Chiaradia

Lefebvre

Parra

et al. 2017

Sci Rep

196

183

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The mycobacterial envelope is unique, containing the so-called mycomembrane (MM) composed of very-long chain fatty acids, mycolic acids (MA). Presently, the molecular composition of the MM remains unproven, due to the diversity of methods used for determining its composition. The plasma membranes (PM) and the native MM-containing cell walls (MMCW) of two rapid-growing mycobacterial species, Mycobacterium aurum and M. smegmatis, were isolated from their cell lysates by differential ultracentrifugation. Transmission electron microscopy and biochemical analyses demonstrated that the two membranes were virtually pure. Bottom-up quantitative proteomics study indicated a different distribution of more than 2,100 proteins between the PM and MMCW. Among these, the mannosyltransferase PimB, galactofuranosyltransferase GlfT2, Cytochrome p450 and ABC transporter YjfF, were most abundant in the PM, which also contain lipoglycans, phospholipids, including phosphatidylinositol mannosides, and only a tiny amount of other glycolipids. Antigen85 complex proteins, porins and the putative transporters MCE protein family were mostly found in MMCW fraction that contains MA esterifying arabinogalactan, constituting the inner leaflet of MM. Glycolipids, phospholipids and lipoglycans, together with proteins, presumably composed the outer leaflet of the MM, a lipid composition that differs from that deduced from the widely used extraction method of mycobacterial cells with dioctylsulfosuccinate sodium.

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“…Of note, in spite of their individual variability in terms of induration size and duration, the animal model displayed highly reproducible. Moreover, several studies have also demonstrated that injection of PPD in guinea pigs was highly sensitive to infection (Dharmadhikari et al, 2011;Moradi et al, 2015) and developed strong TST after infection with Mtb (Kalra et al, 2010). The clinical and pathological changes observed in the infected guinea pigs were similar to those infected through the respiratory or subcutaneous routes in guinea pigs.…”

Section: Discussionmentioning

confidence: 99%

Guinea pig infected with Mycobacterium tuberculosis via oral consumption

Jianfeng

Guo

et al. 2018

Journal of Applied Animal Research

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Guinea pig represents a highly suitable animal model for study of Mycobacterium tuberculosis (Mtb)infection, as it demonstrates similarities to Mtb pulmonary infection in humans. It is known that guinea pigs can be efficiently infected by the respiratory or subcutaneous exposure to Mtb. However, information on Mtb infection through oral route is almost absent in the literature. Here, we examined whether guinea pigs can be infected by drinking Mtb containing water. Our findings confirmed that the guinea pigs could be infected with Mtb via drinking virulent water. The infected guinea pigs developed uniform oval-craterform ulcers at the 30th day after infection. Bacterial cultures showed Mtb growth in the lungs and spleens from the guinea pigs infected with high dose of Mtb. In addition, the infected animals had histopathological granulomatous lesions in lungs, spleens and mesenteric lymph nodes. We provide the compelling evidence that the guinea pigs could be infected by drinking Mtb containing water. The clinical and pathological observations in the infected animals were similar to those found in guinea pigs infected via the respiratory or subcutaneous routes.

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Evaluation of Mycobacterium tuberculosis Early Secreted Antigenic Target 6 Recombinant Protein as a Diagnostic Marker in Skin Test

Cited by 6 publications

References 12 publications

A New Formula for BCG Scar and Tuberculin Test Response

A New Formula for BCG Scar and Tuberculin Test Response

Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane

Guinea pig infected with Mycobacterium tuberculosis via oral consumption

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