Evaluation of mycobacteria growth indicator tubes for susceptibility testing of Mycobacterium tuberculosis to isoniazid and rifampin

Susceptibility Testing with the Manual Mycobacteria Growth Indicator Tube (MGIT) and the MGIT 960 System Provides Rapid and Reliable Verification of Multidrug-Resistant Tuberculosis

“…The INH susceptibility results generated by the MGIT methods were in full agreement with the expected results but disagreed in 3 to 6% of the cases with the BACTEC 460 results. In preliminary studies which compared the manual MGIT and BACTEC 460 methods, highly similar results were obtained (13,14). The INH and RMP susceptibility results obtained in the studies which compared the MGIT 960 and BACTEC 460 methods were also similar to our results (1,3,16).…”

“…The fluorescence can be detected by manual transillumination with a 365-nm UV light, e.g., a Wood's lamp. Preliminary studies have reported that the MGIT method can also be used for AST (4,12,13,14,18).…”

Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis

“…Growth is detected by comparing the fluorescence in the drug-containing tubes and growth control allowing determination of susceptibility or resistance. Several studies have evaluated the MGIT manual system for DST of M. tuberculosis against the first-line drugs INH, RIF, ethambutol (EMB) and streptomycin (SM) with good results (Reisner et al, 1995;Palaci et al, 1996;. These studies found good accuracy for detecting resistance, especially for INH and RIF.…”

Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system

“…The MGIT system is read manually, requiring only a UV lamp, and the tubes are easily inoculated. Preliminary studies of drug susceptibility testing of M. tuberculosis strains have shown promising results (11)(12)(13).…”