Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Abdul-Hafeez, Essam Y.; Karamova, Nazira S.; Ilinskaya, О. N.

doi:10.17816/ecogen16355-61

Cited by 6 publications

(7 citation statements)

References 33 publications

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“…However, bearing in mind that diabetic patients would use the fruit extract for long-term management of symptoms of diabetes, there is a need to determine the mutagenic properties of the fruit extract. While some papers have reported anti mutagenic properties of the stem bark of Kigelia Africana [21], the results may not be generalizable since there could be differences in the chemical composition of the fruit compared to the stem bark.…”

Section: Reported Research Findingsmentioning

confidence: 81%

Commentary on the Antidiabetic Activity of Kigelia Africana

Muyenga-Akapelwa¹,

Ezeala²,

Mushabati³

et al. 2021

JPRM

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Healthcare financing is the process of utilizing financial inputs necessary for health interventions. Health facilities do not routinely access the monthly government grant due to challenges in the access and utilization process. It is unclear if the lack of grant receipt affects strategic health indicators and thus access quality healthcare provision in rural Zambia. This study aimed at investigating the effect of funding access on health facility performance. The study analyzed data for 15 health facilities in Chikankata district from 2014 to 2018. Data was collected from the health management information system and analyzed against accounting records to determine if facilities performed better in antenatal care, Immunization and Institutional deliveries when they received their monthly grants. Grant receipt had a statistically significant (p=0.04) association with performance in maternal and child health indicators, measured by a mean composite score OR 1.46, 95% CI [1.03, 2.08]. The association between grant receipt and indicator performance was most significant on institutional deliveries OR 1.75, 95% [1.13, 2.73]. Grant receipt by the facilities was associated with an improvement in maternal and child health indicators. This demonstrates the need for effective healthcare financing mechanisms that consider the monthly grant in improving performance by facilities. Performance Based Financing mechanisms should however be combined with direct financing mechanisms to holistically improve primary healthcare. The implications of these findings are that as much as practical, funding disbursement modalities must ensure that all health facilities receive some funding

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Section: Reported Research Findingsmentioning

confidence: 81%

Commentary on the Antidiabetic Activity of Kigelia Africana

Muyenga-Akapelwa¹,

Ezeala²,

Mushabati³

et al. 2021

JPRM

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“…The mutant Salmonella typhimurium strain (TA100) that requires histidine for growing in minimal media is suitable for measuring the antimutagenic activity of mutagenic substances. [20][21][22] In this phase, the anti-mutagenic effect of the extract was evaluated by adding S 9 (The sterile extract of the mouse liver containing microsomal enzymes). The cytochrome oxidase enzyme (P450), which inactivates oxidant and toxic compounds, can be found in the membrane of liver cells, especially the endoplasmic reticulum membrane).…”

Section: Anti-mutagenic Activity Assaymentioning

confidence: 99%

“…Then, the experiment was repeated three times, and petri dishes were placed in an incubator (at 37°C for 24 hours). 21,22 The positive control contained NaN 3 (1.5 μg) as a mutagen per plate, and plates without any NaN 3 or the plant extract, which only consisted of 0.5 mL sterile distilled water, were considered as the negative control.…”

Section: Anti-mutagenic Activity Assaymentioning

confidence: 99%

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Investigating Anti-mutagenic Activities of Lantana camara L. (Verbenaceae) Applying Salmonella typhimurium and the Ames Test

Zare

2020

Int J Basic Sci Med

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Introduction: Genetic mutations have a significant role in causing cancers, and plants are effective on cancer recovery by producing metabolites. In this regard, the present study aimed to evaluate the Lantana camera anti-mutation effects applying Salmonella typhimurium in the Ames test. Methods: To this end, the plant was prepared from the Iran National Botanical Garden in 2018 (Tehran, Iran), and the methanolic extracts of its leaves and flowers were obtained by the percolation method. Then, anti-mutagenic activities were studied by the Ames method and the assessment of the rate of reverse mutations in mutant Salmonella typhimurium. Mutant strains cannot grow on minimal mineral media thus only those bacteria that have acquired a wild genotype after reverse mutation in the presence of the mutagen are able to grow on this medium. The plant extract, along with a mutagen substance was used to evaluate its anti-mutagenic effects by counting grown colonies and calculating the mean mutation inhibitory index according to the "Ong" formula. Finally, anti-mutagenic activities were retested by adding the sterile extract of the mouse liver (S9), and the data were analyzed by SPSS statistical software, version 22.Results: In general, the results showed that the mean number of grown colonies decreased significantly despite the plant material in comparison with the standard. According to the "Ong" formula, the percentage of inhibition was [1-T/M]×100. Based on the results, T grew a number of colonies on each petri dish despite the mutagen and extract, and M grew a number of colonies in positive control plates. Eventually, mutation inhibition percentages in leaf extracts were significantly higher than those of flower extracts, which were 75.59 ± 0.73 (+S9) and 84.79 ± 0.17 (-S9), as well as 49.57 ± 0.55 (+S9) and 62.32 ± 0.23 (-S9), respectively (P < 0.05). Conclusion: In general, the leaves and flowers of L. camara demonstrated anti-mutagenic activities with higher activities in the leaves compared to flowers.

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“…Since all living organisms have chemically similar DNA, chemicals that cause mutations in bacteria may also cause mutations in humans. As a result, bacteria can be used as a diagnostic tool to identify mutagenic substances that may cause cancer in humans without the need to wait a long time for the development of cancer in the living organism [7,8]. A novel bacterial system has been developed in the current study using auxotroph's tryptophan Escherichia coli.…”

mentioning

confidence: 99%

Design of a bacterial system using Escherichia coli to detect the mutagenic effect of some drugs

Hezam¹

2023

QJPS

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The current study aimed to use the auxotroph's tryptophan Escherichia coli as a test to detect Ibuprofen's mutagenic potential. the Ames test was used to detect reverse mutations in auxotroph's E. coli treated with Ibuprofen. The results showed a significant increase (P< 0.05) in reverse colonies of auxotroph's E. coli treated with 250 and 500 mg/mL of Ibuprofen compared to the negative control. The polymerase chain reaction and DNA Sequencing were used to detect the reverse mutations in the try gene of the auxotroph's E. coli. The results showed the presence of the try gene in all isolates treated with 250 and 500 mg/mL of Ibuprofen at a percentage of (100%). The try gene was sequenced using BLAST software and compared to the gene sequence of a standard isolate. The genetic variation analysis of the try gene isolates treated with Ibuprofen revealed that point mutations in the DNA of the try gene altered protein translation.

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Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Cited by 6 publications

References 33 publications

Commentary on the Antidiabetic Activity of Kigelia Africana

Commentary on the Antidiabetic Activity of Kigelia Africana

Investigating Anti-mutagenic Activities of Lantana camara L. (Verbenaceae) Applying Salmonella typhimurium and the Ames Test

Design of a bacterial system using Escherichia coli to detect the mutagenic effect of some drugs

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