Evaluation of multiple genetic markers for typing drug-resistant Mycobacterium tuberculosis strains from Poland

Sajduda, Anna; Dziadek, Jarosław; Kotłowski, Roman; Portaels, Françoise

doi:10.1016/j.diagmicrobio.2005.12.004

Cited by 15 publications

(17 citation statements)

References 22 publications

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“…The calculated discriminatory power of this method was higher than those of spoligotyping and MIRU-VNTR typing and almost equivalent to that of IS6110-RFLP typing (406)(407)(408).…”

Section: Methods Based On Repetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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“…The calculated discriminatory power of this method was higher than those of spoligotyping and MIRU-VNTR typing and almost equivalent to that of IS6110-RFLP typing (406)(407)(408).…”

Section: Methods Based On Repetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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“…Our results have shown the importance of a combined strategy for M. tuberculosis typing, in which clusters defined upon spoligotyping would be subject to further differentiation using methods with high discriminatory power. 33,42 Spoligotyping and IS6110-Mtb1-Mtb2 PCR produced clustering rates of 75% and 39%, respectively. The combination of these 2 methods provided certain and reliable evidence of the relationship of the tested strains.…”

Section: -39mentioning

confidence: 99%

“…19, 33,34 Increasing differentiating power of the genetic methods used in the study correlated with the reduced number of strains constituting possible epidemiological groups. In spoligotyping, such groups were composed of 157 strains (75%), whereas in IS6110-Mtb1-Mtb2 PCR, the number of strains constituting potential epidemic groups was reduced to 82 (39%).…”

Section: -10mentioning

confidence: 99%

Transmission of tuberculosis among people living in the border areas of Poland, the Czech Republic, and Slovakia

Kozińska¹,

Zientek²,

Augustynowicz–Kopeć³

et al. 2019

Polish Archives of Internal Medicine

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“…Although the IS 6110 -Mtb1-Mtb2 PCR method has not been exploited sufficiently, the results from the insofar performed studies suggest its usefulness in the molecular epidemiological investigations of TB. Most notably the discriminatory power of this method has been demonstrated as almost equivalent to that of IS 6110 -RFLP typing [67, 148, 149]. …”

Section: Methods Based On Gc-rich Sequencesmentioning

confidence: 99%

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

Jagielski

Ingen

Rastogi

et al. 2014

BioMed Research International

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In the epidemiology of tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases, as in all infectious diseases, the key issue is to define the source of infection and to disclose its routes of transmission and dissemination in the environment. For this to be accomplished, the ability of discerning and tracking individual Mycobacterium strains is of critical importance. Molecular typing methods have greatly improved our understanding of the biology of mycobacteria and provide powerful tools to combat the diseases caused by these pathogens. The utility of various typing methods depends on the Mycobacterium species under investigation as well as on the research question. For tuberculosis, different methods have different roles in phylogenetic analyses and person-to-person transmission studies. In NTM diseases, most investigations involve the search for environmental sources or phylogenetic relationships. Here, too, the type of setting determines which methodology is most suitable. Within this review, we summarize currently available molecular methods for strain typing of M. tuberculosis and some NTM species, most commonly associated with human disease. For the various methods, technical practicalities as well as discriminatory power and accomplishments are reviewed.

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Evaluation of multiple genetic markers for typing drug-resistant Mycobacterium tuberculosis strains from Poland

Cited by 15 publications

References 22 publications

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Transmission of tuberculosis among people living in the border areas of Poland, the Czech Republic, and Slovakia

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

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