Evaluation of morphological parameters of bone formation in Sprague–Dawley rats of different ages by<i>in vivo</i>fluorochrome labeling

Lu, Hongmei; Cui, Liao; Zuo, Changqing; Lin, Sien; Wu, Tie

doi:10.1080/11250003.2014.984781

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…) through increased connectivity as observed in our study. Our findings for BV/TV, Tb.Sp, Tb.Th, and Tb.N are in agreement with other studies, where similar patterns also were observed for loaded and normal bone growing process for aged rats and mice bone structure.…”

Section: Discussionsupporting

confidence: 93%

“…(83) Indeed, it has been shown that exercised animals have beneficial effects on BV/TV compared with nonexercised animals. (71,84) A significant change in Tb.Th is an indication of loading-induced positive effects on normal bone-growing phenomena, (85) and the change in Tb.N is directly associated with the change in BV/TV and Tb.Th. (86) This explains why the MI group showed discontinued positive effects on Tb.N after 14 weeks of age, simultaneously with BV/TV and Tb.Th (Fig.…”

Section: Cyclic Loading Temporarily Reduced Body Weight and Food Intamentioning

confidence: 99%

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Isolated Cyclic Loading During Adolescence Improves Tibial Bone Microstructure and Strength at Adulthood

et al. 2020

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Bone is a unique living tissue, which responds to the mechanical stimuli regularly imposed on it. Adolescence facilitates a favorable condition for the skeleton that enables the exercise to positively influence bone architecture and overall strength. However, it is still dubious for how long the skeletal benefits gained in adolescence is preserved at adulthood. The current study aims to use a rat model to investigate the effects of in vivo low‐ (LI), medium‐ (MI), and high‐ (HI) intensity cyclic loadings applied during puberty on longitudinal bone development, morphometry, and biomechanics during adolescence as well as at adulthood. Forty‐two young (4‐week‐old) male rats were randomized into control, sham, LI, MI, and HI groups. After a 5 day/week for 8 weeks cyclic loading regime applied on the right tibia, loaded rats underwent a subsequent 41‐week, normal cage activity period. Right tibias were removed at 52 weeks of age, and a comprehensive assessment was performed using μCT, mechanical testing, and finite element analysis. HI and MI groups exhibited reduced body weight and food intake at the end of the loading period compared with shams, but these effects disappeared afterward. HI cyclic loading increased BMD, bone volume fraction, trabecular thickness, trabecular number, and decreased trabecular spacing after loading. All loading‐induced benefits, except BMD, persisted until the end of the normal cage activity period. Moreover, HI loading induced enhanced bone area, periosteal perimeter, and moment of inertia, which remained up to the 52nd week. After the normal cage activity at adulthood, the HI group showed increased ultimate force and stress, stiffness, postyield displacement and energy, and toughness compared with the sham group. Overall, our findings suggest that even though both trabecular and cortical bone drifted through age‐related changes during aging, HI cyclic loading performed during adolescence can render lifelong benefits in bone microstructure and biomechanics. © 2020 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

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Section: Discussionsupporting

confidence: 93%

Section: Cyclic Loading Temporarily Reduced Body Weight and Food Intamentioning

confidence: 99%

Isolated Cyclic Loading During Adolescence Improves Tibial Bone Microstructure and Strength at Adulthood

et al. 2020

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“…Also, another study reported a greater BV/TV in the growing rat tibiae after 8-weeks of free fall exercise routine 70 . The observed increment in Tb.Th during the loading period is a part of normal bone development 71 . However, a significant increment in the loaded limbs (compared to shams) indicated an additional improvement in trabecular structure under impact loading conditions.…”

Section: Discussionmentioning

confidence: 99%

High Impact Exercise Improves Bone Microstructure and Strength in Growing Rats

Mustafy

Londono

Moldovan

et al. 2019

Sci Rep

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Physical activity is beneficial for skeletal development. However, impact sports during adolescence, leading to bone growth retardation and/or bone quality improvement, remains unexplained. This study investigated the effects of in vivo low (LI), medium (MI), and high (HI) impact loadings applied during puberty on bone growth, morphometry and biomechanics using a rat model. 4-week old rats (n = 30) were divided into control, sham, LI, MI, and HI groups. The impact was applied on the right tibiae, 5 days/week for 8 weeks mimicking walking (450 µε), uphill running (850 µε) and jumping (1250 µε) conditions. Trabecular and cortical parameters were determined by micro-CT, bone growth rate by calcein labeling and toluidine blue staining followed by histomorphometry. Bio-mechanical properties were evaluated from bending tests. HI group reduced rat body weight and food consumption compared to shams. Bone growth rate also decreased in MI and HI groups despite developing thicker hypertrophic and proliferative zone heights. HI group showed significant increment in bone mineral density, trabecular thickness, cortical and total surface area. Ultimate load and stiffness were also increased in MI and HI groups. We conclude that impact loading during adolescence reduces bone growth moderately but improves bone quality and biomechanics at the end of the growing period.

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“…As for Tb.Th, Tb.N, and Tb.Sp, no difference was found between the irradiated and control tibiae for the 0.83 Gy group ( Fig 6 ), while Tb.Th and Tb.N were significantly increased in the growing period for this group ( Table 2 ). The increment of Tb.Th during the growing period indicates normal bone growth process [ 24 , 60 , 61 ]. Moreover, the observed increase in Tb.N is associated with the concomitant increase in BV/TV for the young age period [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Can repeated in vivo micro-CT irradiation during adolescence alter bone microstructure, histomorphometry and longitudinal growth in a rodent model?

et al. 2018

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In vivo micro-computed tomography (micro-CT) can monitor longitudinal changes in bone mass and microstructure in small rodents but imposing high doses of radiation can damage the bone tissue. However, the effect of weekly micro-CT scanning during the adolescence on bone growth and architecture is still unknown. The right proximal tibia of male Sprague-Dawley rats randomized into three dose groups of 0.83, 1.65 and 2.47 Gy (n = 11/group) were CT scanned at weekly intervals from 4th to 12th week of age. The left tibia was used as a control and scanned only at the last time point. Bone marrow cells were investigated, bone growth rates and histomorphometric analyses were performed, and bone structural parameters were determined for both left and right tibiae. Radiation doses of 1.65 and 2.47 Gy affected bone marrow cells, heights of the proliferative and hypertrophic zones, and bone growth rates in the irradiated tibiae. For the 1.65 Gy group, irradiated tibiae resulted in lower BMD, Tb.Th, Tb.N and a higher Tb.Sp compared with the control tibiae. A decrease in BMD, BV/TV, Tb.Th, Tb.N and an increase in Tb.Sp were observed between the irradiated and control tibiae for the 2.47 Gy group. For cortical bone parameters, no effects were noticed for 1.65 and 0.83 Gy groups, but a lower Ct.Th was observed for 2.47 Gy group. Tibial bone development was adversely impacted and trabecular bone, together with bone marrow cells, were negatively affected by the 1.65 and 2.47 Gy radiation doses. Cortical bone microstructure was affected for 2.47 Gy group. However, bone development and morphometry were not affected for 0.83 Gy group. These findings can be used as a proof of concept for using the reasonable high-quality image acquisition under 0.83 Gy radiation doses during the adolescent period of rats without interfering with the bone development process.

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Evaluation of morphological parameters of bone formation in Sprague–Dawley rats of different ages byin vivofluorochrome labeling

Cited by 9 publications

References 15 publications

Isolated Cyclic Loading During Adolescence Improves Tibial Bone Microstructure and Strength at Adulthood

Isolated Cyclic Loading During Adolescence Improves Tibial Bone Microstructure and Strength at Adulthood

High Impact Exercise Improves Bone Microstructure and Strength in Growing Rats

Can repeated in vivo micro-CT irradiation during adolescence alter bone microstructure, histomorphometry and longitudinal growth in a rodent model?

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