Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples

Chaivisuthangkura, Parin; Pengsuk, Chalinan; Longyant, Siwaporn; Sithigorngul, Paisarn

doi:10.1016/j.mimet.2013.09.013

Cited by 17 publications

(17 citation statements)

References 34 publications

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“…Through these test procedures, the result could be obtained within approximately 20 min after the sample had been introduced. Several methods for V. cholerae detection that have been published recently show a wide range of detection limits, for example, 10-100 CFU via PCR-based method by Mehrabadi et al [26], 10 CFU using a dipstick test by Chakraborty et al [27], and 5 × 10 5 cfu/mL through an immunochromatographic strip test by Chaivisuthangkura et al [28]. However, these limits could not be compared due to the different target analytes, detection reporters and antibody system used in each assay.…”

Section: Dot Fluorescence Immunoassay Stripmentioning

confidence: 99%

Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip

Thepwiwatjit

Thattiyaphong

Limsuwan

et al. 2014

Journal of Nanomaterials

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This paper describes an application of Rubpy dye-doped silica nanoparticles (RSNPs) as signal reporter in a dot fluorescence immunoassay strip for rapid screening ofVibrio choleraO1 (VCO1). These nanoparticles have a spherical shape with an average diameter of 45 nm. They appear luminescent orange when excited with a 312 nm UV lamp. Based on the sandwich immunoassay principle, a test strip was made of a nitrocellulose membrane dotted with monoclonal antibodies against VCO1 as analyte capture molecules. After introducing a test sample, followed by polyclonal rabbit anti-VCO1 antibody conjugated RSNPs as detection reporters and one washing step, the presence or absence of the target bacteria could be identified under UV light by naked eyes. A positive sample would signal a bright orange dot on the strip. The proposed assay had a detection limit of4.3×103 cfu/mL and was successfully applied as a rapid screening test for VCO1 in food samples with high sensitivity, specificity, and accuracy.

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Section: Dot Fluorescence Immunoassay Stripmentioning

confidence: 99%

Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip

Thepwiwatjit

Thattiyaphong

Limsuwan

et al. 2014

Journal of Nanomaterials

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“…However, at 30 days, both dilutions of bacterial suspension showed lighter immunoreactivity (Figure ). This result indicated that the strip could remain function under storage at room temperature equivalent to at least 2 years (Paek, Lee, Cho, & Kim, ), which is also similar to the shelved lives of strips for white spot syndrome virus (Wangman, Siriwattanarat, et al, ), yellow‐head virus (Sithigorngul et al, ), infectious myonecrosis virus (Wangman et al, ), V. cholerae O1 (Chaivisuthangkura et al, ) and V. cholerae O139 (Pengsuk et al, ).…”

Section: Resultsmentioning

confidence: 57%

“…hepatopancreas, stomach, faecal) and environment materials, sample enrichment was needed before PCR analysis to increase VP AHPND detection effectiveness. After enrichment for 4–6 hr, the broth could be directly used as a PCR template (Han, Tang, Piamsomboon, & Pantoja, ; Sirikharin et al, ) and the detection limit of the bacteria was approximately 10 4 cfu/ml (Sirikharin et al, ), which was close to the detection limit of strip tests of V. cholerae O1 and O139 (Chaivisuthangkura et al, ; Pengsuk et al, ).…”

Section: Resultsmentioning

confidence: 90%

“…A similar result was obtained using the bacterial suspension, in which the strip could detect the bacteria at 10 7 cfu/ml, which is equivalent of a sensitivity 10 times lower than dot blotting (10 6 cfu/ml, Figures and ). In strip tests for the detection of V. cholerae O1 (Chaivisuthangkura, Pengsuk, Longyant, & Sithigorngul, ) and V. cholerae O139 (Pengsuk, Chaivisuthangkura, Longyant, & Sithigorngul, ), the detection limit was similar or better than the dot blot method. Therefore, it is hoped that we can increase the sensitivity of the strip to match or outperform the sensitivity of the dot blot method by producing high‐sensitivity MAbs that bind to far distant epitopes on the antigen (Sithigorngul, Rukpratanporn, Chaivisuthangkura, Sridulyakul, & Longyant, ; Wangman, Siriwattanarat, et al, ).…”

Section: Resultsmentioning

confidence: 98%

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Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease

Wangman

Chaivisuthangkura

Taengchaiyaphum

et al. 2019

Journal of Fish Diseases

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Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VP AHPND ), were employed to develop a rapid strip test. One MAb was conjugated to colloidal gold to bind to ToxB at the application pad, and another MAb was used to capture colloidal gold MAb-protein complexes at the test line (T) on the nitrocellulose strip. To validate test performance, a downstream control line (C) of How to cite this article: Wangman P, Chaivisuthangkura P, Taengchaiyaphum S, Pengsuk C, Sithigorngul P, Longyant S. Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease. J Fish Dis.

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“…However, for food sample analysis, IFIs have higher false‐positive rates than with ELISA and PCR (Bohaychuk and others ), and their low sensitivity sometimes delays the results due to cultural enrichment necessity (Shim and others ; Sithigorngul and others ). A strip test using AuNPs as indicators for the detection of V. cholerae O1 was developed and evaluated in spiked seafood samples (Chaivisuthangkura and others ). Without cultural enrichment, bacterial concentrations of 5 × 10 5 to 10 6 CFU/mL in seafood samples were detected.…”

Section: Immunological‐based Methodsmentioning

confidence: 99%

Culture‐Independent Rapid Detection Methods for Bacterial Pathogens and Toxins in Food Matrices

Wang

Salazar

2015

Comp Rev Food Sci Food Safe

221

152

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Rapid detection of bacterial pathogens and toxins in foods is necessary to provide real-time results to mitigate foodborne illness outbreaks. Cultural enrichment methods, although the most widely used, are time-consuming and therefore inadequate for rapid pathogen detection from food samples. The development of novel "rapid" detection methods has decreased detection time dramatically. This review presents an overview of detection methods for various foodborne pathogens, including Listeria monocytogenes, Salmonella enterica, and shiga toxin-producing Escherichia coli, and bacterial toxins in food matrices, with emphasis on those methods which do not require cultural enrichment. Discussed methods include nucleic acid-, immunological-, and biosensor-based techniques. A summary of each type of detection method is given, including referenced methods from the literature. Since these discussed methods do not require cultural enrichment, there is a higher probability of interference from the food matrices. Therefore, the review also discusses the potential interference of food components on detection methods and addresses preprocessing strategies to overcome matrix associated inhibition and to concentrate low quantities of pathogens and toxins in food. Development of rapid and sensitive detection technologies advances and ensures public health safety and security.

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Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples

Cited by 17 publications

References 34 publications

Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip

Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip

Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease

Culture‐Independent Rapid Detection Methods for Bacterial Pathogens and Toxins in Food Matrices

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