Evaluation of Monoclonal Antibodies with Specificity for the Human Platelet HPA-1 Allotypes

Abstract

Two mouse monoclonal antibodies have been characterized. Both antibodies specifically recognized the amino acid sequences which are responsible for the presence of the HPA-la and HPA-lb alloantigens at the surface of human platelet. The monoclonality allied with an exquisite specificity of these antibodies allow a perfect typing of platelet HPA-1 status. A fast and reliable Elisa assay which fulfills all the needs and requirements of hematology laboratories has been set up and is described here. The Elisa assay has been tested on a population of 689 blood donors. The results agreed with the known phenotypic frequencies of the HPA-1 alloantigens.

A rapid and specific whole blood HPA‐1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb‐IIIa complexes

“…The use of MoAbs has already been described to discriminate HPA‐1a/1a from HPA‐1a/1b phenotypes. Indeed, Diaz et al (1993) developed a reliable enzyme‐linked immunosorbent assay (ELISA) using two well‐characterized MoAbs for HPA‐1 phenotyping. However, the ELISA is longer than our flow cytometric assay, which could give a result within 1 h after blood sampling.…”