Evaluation of molecular typing for epidemiological study of Chlamydia trachomatis genital infections

Rodríguez, Patricia; Barbeyrac, Bertille de; Persson, Kenneth; Dutilh, B; Bebear, Cécile

doi:10.1128/jcm.31.8.2238-2240.1993

Cited by 42 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the difference between the two genders is the greatest for genotype E and F, the difference does not reach statistical significance (Pearson's chi-square test: genotype E, P = 0.1122 a = 0.05; genotype F, P = 0.2568, a = 0.05) for either of the genotypes. Our results are in concordance with several studies all over the world that assessed unselected or selected populations (Rodriguez et al, 1993;Ikehata et al, 2000;Millman et al, 2004;Gao et al, 2007;Mossman et al, 2008;Jurstrand et al, 2010;Machado et al, 2011) as shown in (Lee et al, 2006;Gao et al, 2007), pregnant women (Ikehata et al, 2000), and women suffering urethritis or cervicitis (Zheng et al, 2007) found genotype E to be dominant. Regardless of the difference in the most prevalent genotype found in a specific study group, all studies including our own seem to be in concordance that genotypes D, E, and F are the most common genotypes in urogenital chlamydial infections as found by Naher & Petzoldt (1991) as early as 20 years ago.…”

Section: Discussionsupporting

confidence: 93%

Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay

Keše

Potočnik

Matičič

et al. 2011

FEMS Immunol Med Microbiol

View full text Add to dashboard Cite

The aim of the present study was to develop and validate a multitarget pyrosequencing-based protocol for basic Chlamydia trachomatis genotyping directly from clinical samples and to characterize the distribution of genotypes among Slovenian sexually active population. The newly developed combination of assays that targets the variable domains VD-I and VD-IV of the C. trachomatis ompA gene, was optimized and validated with 11 reference C. trachomatis strains and by comparison to complete ompA conventional sequencing. In addition, 183 clinical specimens which were previously diagnosed as C. trachomatis positive were evaluated by pyrosequencing. The pyrosequencing products showed a 100% match to corresponding sections of the respective conventional ompA sequences. Based on our results the most frequent genotype in urogenital samples was E (51.1%) followed by F (21.4%), G and K (6.9%), D (6.1%), H (3.8%), J (2.3%) and Ia and Ja (0.8%). In conjunctiva samples the genotype distribution was E (63.3%), D and F (13.3%), K (6.7%) and G (3.3%). Pyrosequencing thus proved itself to be a rapid method for C. trachomatis typing, which is important for better understanding the pathogenesis and epidemiology of this pathogen.

show abstract

Section: Discussionsupporting

confidence: 93%

Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay

Keše

Potočnik

Matičič

et al. 2011

FEMS Immunol Med Microbiol

View full text Add to dashboard Cite

show abstract

“…A study also pointed out that type E was not only the most prevalent type but also very stable, with few changes even between strains of different geographical origins, as illustrated by isolates collected in France and Sweden (Rodriguez et al, 1993). It was subsequently shown that PCR could be made directly from cervical scrapes (Lan et al, 1993), which made typing possible for laboratories without access to culture facilities.…”

Section: Molecular Methods: Omp1mentioning

confidence: 99%

TypingChlamydia trachomatis: from egg yolk to nanotechnology

Pedersen

Herrmann

Frimodt‐Møller³

2009

FEMS Immunology &Amp; Medical Microbiology

View full text Add to dashboard Cite

A historical review is provided of the various methods used for half a century to differentiate and type Chlamydia trachomatis strains. Typing of C. trachomatis is an important tool for revealing transmission patterns in sexual networks, and enabling association with clinical manifestations and pathogenicity. Serotyping using the major outer membrane protein (MOMP) has been the mainstay of epidemiological work for several decades. However, the development of nucleic acid amplification techniques (NAAT) and easy access to sequencing have shifted the focus from MOMP serotypes to omp1 genotypes. However, insufficient epidemiological resolution is achieved by characterization of both MOMP and omp1. This calls for new high-resolution genotyping methods applying for example a multilocus variable number tandem repeat assay (MLVA) or multilocus sequence typing (MLST). The futuristic nanotechnology already seems at hand to further simplify and automate the high-resolution genotyping method based on NAAT and sequencing of various targets in the C. trachomatis genome. Thereby, a high throughput can be achieved and more epidemiological information can be obtained. However, it is important to realize that culture of C. trachomatis may still be needed to detect and characterize new variants of C. trachomatis.

show abstract

“…PCK amplification and RFLP analysis were used to detect and characterize serovars of C. trachomatic as described previously [5,0]. From nasopharyngeal specimens, IINA was extracted in lysis buffer (10 mM Tris-HC1, Tween-20 0.5'%, 1 (Milligan Chrp., Kedford, MA, USA) and purified by high-pressure liquid chromatography.…”

Section: Pcr-rflp Analysismentioning

confidence: 99%

Unclassified serovars of Chlamydia trachomatis isolated from Japanese infants

Numazaki

Ikehata

Chiba

et al. 1998

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Objective: To analyze antigenic and genetic variations of Chlamydia trachomatis among the serovars obtained from Japanese infants. Methods:The polymerase chain reaction (PCR) was used to amplify a large part of the major outer-membrane protein gene, and restriction fragment length polymorphism (RFLP) was used to identify the serovars of C. trachomatis from nasopharyngeal and conjunctival swabs from Japanese infants and neonates. Results:The typing of 10 nasopharyngeal isolates gave the following results: seven E, one H, and two unclassified serovars. The typing of seven conjunctival isolates gave the following results: five D, one F, and one unclassified serovar. Reactive patterns of these unclassified strains, determined by PCR-RFLP, to monoclonal antibodies were different from those of 15 reference serovars.Conclusions: Characterization of unclassified variants will allow more detailed epidemiologic studies of perinatal C. trachomatis infections i n Japan.

show abstract

Evaluation of molecular typing for epidemiological study of Chlamydia trachomatis genital infections

Cited by 42 publications

References 13 publications

Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay

Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay

TypingChlamydia trachomatis: from egg yolk to nanotechnology

Unclassified serovars of Chlamydia trachomatis isolated from Japanese infants

Contact Info

Product

Resources

About