Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics

Ji, Chenglong; Lu, Zhen; Xu, Lanlan; Li, Fēi; Cong, Ming; Shan, Xiujuan; Wu, Huifeng

doi:10.1016/j.envpol.2019.05.046

Cited by 39 publications

(25 citation statements)

References 73 publications

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“…Mitochondrial toxicity of Cd has been shown in clams (Ji et al, 2019), mitochondrial dysfunctions in mussel hemocytes are induced after an exposure to titanium particles (Wang et al, 2019) and infection of B. glabrata with nematodes induces suppression of mitochondrial oxidative metabolism (Tunholi-Alves et al, 2019). In this sense, we believe that investigating the consequences of environmental information on cellular metabolism is of great interest to first investigate the link between the environment and DNA methylation.…”

Section: 3-dna Methylation Is Responsive To the Environment In Mollmentioning

confidence: 99%

Epigenetic inheritance and intergenerational effects in mollusks

Fallet

Luquet

David

et al. 2020

Gene

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List of abbreviations 5mC5-methylcytosine, a cytosine that has been modified by the addition of a methyl group on its 5th carbon ADP Adenosine diphosphate AFLP Amplified fragment length polymorphism BLAST Basic Local Alignment Search Tool BS-Seq Bisulfite sequencing BS-PCR-Seq PCr amplification after bisulfite sequencing followed by sequencing Cd Cadium meDIP-seq Methylated DNA Immunoprecipitation sequencing MiRNAs Micro RNAs MSAP Methylation-sensitive amplified polymorphism MS-PCR Methylation-specific PCR pCO2 partial pressure of C02 PCR Polymerase chain reaction PTMs Post-Translational Modifications pH potential of Hydrogen qPCR quantitative Polymerase chain reaction RT-qPCR quantitative reverse transcription PCR RNA Ribonucleic acid RRBS Reduced representation bisulfite sequencing SAM S-adenosyl methionine SDS-PAGE Sodium Dodecil Sulfate PolyAcrylamide Gel Electrophoresis UV-HPLC Ultraviolet High performance liquid chromatography Abstract Recent insights in evolutionary biology have shed light on epigenetic variation that interacts with genetic variation to convey heritable information. An important characteristic of epigenetic changes is that they can be produced in response to environmental cues and passed on to later generations, potentially facilitating later genetic adaptation. While our understanding of epigenetic mechanisms in vertebrates is rapidly growing, our knowledge about invertebrates remains lower, or is restricted to model organisms. Mollusks in particular, are a large group of invertebrates, with several species important for ecosystem function, human economy and health. In this review, we attempt to summarize the literature on epigenetic and 4 intergenerational studies in mollusk species, with potential importance for adaptive evolution. Our review highlights that two molecular bearers of epigenetic information, DNA methylation and histone modifications, are key features for development in mollusk species, and both are sensitive to environmental conditions to which developing individuals are exposed. Further, although studies are still scarce, various environmental factors (e.g. predator cues, chemicals, parasites) can induce intergenerational effects on the phenotype (life-history traits, morphology, behaviour) of several mollusk taxa. More work is needed to better understand whether environmentally-induced changes in DNA methylation and histone modifications have phenotypic impacts, whether they can be inherited through generations and their role in intergenerational effects on phenotype. Such work may bring insight into the potential role of epigenetic in adaptation and evolution in mollusks.

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Section: 3-dna Methylation Is Responsive To the Environment In Mollmentioning

confidence: 99%

Epigenetic inheritance and intergenerational effects in mollusks

Fallet

Luquet

David

et al. 2020

Gene

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“…Recently, omics approaches in system biology, such as transcriptomics, proteomics and metabolomics, have been proposed to supervise the terrestrial or marine ecosystems and these methods have been used to elucidate the potential toxicity caused by toxic substances to organisms (Chen et al, 2018a,b;García-Sevillano et al, 2013;Ji et al, 2019;Williams et al, 2011). Metabolomics focuses exactly on the alterations of low molecular metabolites (<1000 Da) within cells, tissues or biofluids in organisms (Song et al, 2018;Viant et al, 2003) and offers fresh insights into the toxicological effects of environmental stressors (Jones et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

et al. 2019

Environmental Pollution

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Arsenic (As) is a metalloid element that is ubiquitous in the marine environment and its contamination has received worldwide attention due to its potential toxicity. Arsenic can induce multiple adverse effects, such as lipid metabolism disorder, immune system dysfunction, oxidative stress and carcinogenesis, in animals. Inorganic arsenic includes two chemical forms, arsenite (As (III)) and arsenate (As (V)), in natural environment. As (V) is the dominant form in natural waters. In the present study, metabolomic and proteomic alterations were investigated in juvenile rockfish Sebastes schlegelii exposed to environmentally relevant concentrations of As (V) for 14 d. The analysis of iTRAQ-based proteomics combined with untargeted NMR-based metabolomics indicated apparent toxicological effects induced by As (V) in juvenile rockfish. In details, the metabolites, including lactate, alanine, ATP, inosine and phosphocholine were significantly altered in As-treated groups. Proteomic responses suggested that As (V) could not only affected energy and primary metabolisms and signal transduction, but also influenced cytoskeleton structure in juvenile rockfish. This work suggested that the combined proteomic and metabolomic approach could shed light on the toxicological effects of pollutants in rockfish S. schlegelii.

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“…Mitochondria play key roles in ATP production, apoptosis, calcium cycle, fatty acid metabolism, amino acid metabolism, and heme biosynthesis, which have been frequently reported as the target of contaminant-induced toxicity (Ji et al, 2019;Meyer et al, 2013;Sokolova et al, 2005). As the complex I and III of oxidative phosphorylation, NADH dehydrogenase and cytochrome c reductase are vital in electron transporting.…”

Section: Metabolismmentioning

confidence: 99%

“…Omics technologies based on high-throughput analysis have the ability to provide a global view of the cellular processes of organisms in response to environmental changes, such as pollutant stimulus, and hence uncover the toxicological mechanism of pollutant (Zhang et al, 2018). Especially, integrated omics approach that enables to provide more abundant information at transcript, protein, and metabolite levels has been frequently used in ecology and toxicity researches (Ji et al, 2019;Poulson-Ellestad et al, 2014;Sun et al, 2016a). To identify molecular events and pathways potentially involved in TCPP toxicities, proteomic and metabolomic profiles in rockfish treated with TCPP were evaluated by isobaric tags for relative and absolute quantification (iTRAQ) and nuclear magnetic resonance (NMR) techniques, respectively.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics

Cited by 39 publications

References 73 publications

Epigenetic inheritance and intergenerational effects in mollusks

Epigenetic inheritance and intergenerational effects in mollusks

Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

Global responses to tris(1-chloro-2-propyl)phosphate (TCPP) in rockfish Sebastes schlegeli using integrated proteomic and metabolomic approach

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