Evaluation of miRNA 223/125a and COBLL1 Expressions and ROR-1 Levels as Reliable Markers in B- chronic Lymphocytic Leukemia

Abstract

Background: miRNA 223 /125a and Cordon-bleu Protein Like 1 (COBLL1) are novel biomarkers that can predict prognosis and guide treatment decisions in patients with chronic lymphocytic leukemia (CLL). Also, there is a growing interest in CLL monitoring based on flow cytometry of receptor tyrosine kinase-like orphan receptor-1 (ROR-1).Objective: This study aimed to evaluate the relationship between miRNA 223 /125a and COBLL1 expressions and ROR-1 expression in patients with CLL. Also, the study evaluated the relationship between the expression of these biomarkers with tumor staging and cancer progression. Methods: Our study included 40 patients newly diagnosed with B-CLL. In peripheral blood (PB), miRNA 223/125a and COBLL1 expressions were detected by real-time polymerase chain reaction (real-time PCR) and ROR-1 percentage was detected by flow cytometry before and after treatment. Results: High level of COBLL1 expression was statistically significantly associated with high ROR-1 percentage expression (P= 0.03). However, a high level of miRNA 223/125a expression was statistically significantly associated with low ROR-1 percentage expression (P=0.002). The sensitivity and specificity of ROR-1 as a predictor of high WBCs count after treatment were 96.6 and 81.1%, respectively. There was a statistically significant reduction of ROR-1 percentage after treatment compared to before treatment (P <0.001). Conclusion: ROR-1 percentage expression can be considered a possible prognostic predictor in CLL along with miRNA 223/125a and COBLL1 expressions. This can be explained by the significant correlation between ROR-1 and the studied molecular biomarkers; miRNA 223/125a and COBLL1. In addition, there was a significantly higher ROR-1 percentage in patients with higher WBC counts. Moreover, there was a significant reduction in ROR-1 percentage after treatment.

MicroRNAs: Potential prognostic and theranostic biomarkers in chronic lymphocytic leukemia

“…suggested miRNA 223/125a and Cordon‐bleu Protein Like 1 (COBLL1) as other prognosis predictive markers, since high levels of COBLL1 expression were shown to be significantly correlated with high levels of ROR‐1 (receptor tyrosine kinase‐like orphan receptor‐1) expression in a novel flowcytometry‐based CLL monitoring. However, low ROR‐1 percentage expression was statistically substantially correlated with high levels of miRNA 223/125a expression [134]. Furthermore, using a bioinformatics prediction approach, Bagheri et al.…”

Gene–Nutrient Interactions in Obesity: COBLL1 Genetic Variants Interact with Dietary Fat Intake to Modulate the Incidence of Obesity

“…COBLL1 (cordon-bleu WH2 repeat protein-like 1) gene is associated with neural tube formation [ 23 ], central obesity [ 24 ], fasting insulin [ 25 ], type 2 diabetes risk [ 26 ], blood lipids [ 27 ], CVD risk [ 28 ], gastric cancer [ 29 ], prostate cancer [ 30 ], and leukemia [ 31 , 32 ]. COBLL1 rs6717858 is associated with plasma leptin levels secreted from adipose tissue and obesity-related factors, such as body mass index (BMI), central obesity, waist circumference (WC), fat mass, and lipid levels (prominent effect in women) [ 26 , 27 , 33 , 34 , 35 ].…”