Evaluation of miR-181b and miR-126-5p expression levels in T2DM patients compared to healthy individuals: Relationship with NF-κB gene expression

Dehghani, Mohammadreza; Zarch, Seyed Mohsen Aghaei; Mehrjardi, Mohammad Yahya Vahidi; Nazari, Majid; Babakhanzadeh, Emad; Ghadimi, Hamidreza; Zeinali, Fahime; Talebi, Mehrdad

doi:10.1016/j.endien.2020.09.001

Cited by 2 publications

(3 citation statements)

References 28 publications

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“…The significance of circulating miRNA is two-fold, as they are potential non-invasive markers and mediators of crosstalk between multiple signalling pathways and act differently in physiological and pathological conditions. 20 The findings of this study suggest that miR-181b-5p and miR-330- 3p participate in the development of T2DM through an interconnected network. The miR-181 family is a critical regulator of AT function.…”

Section: Discussionmentioning

confidence: 63%

Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus

Roy

Purohit

Khokhar

et al. 2023

Journal of Obesity & Metabolic Syndrome

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Background: Growth differentiation factor-15 (GDF-15) is involved in insulin resistance and diabetes. In this study, we determine the associations of GDF-15 with miR-181b-5p, miR-330-3p, SMAD7, and insulin resistance in visceral adipose tissue (VAT) and peripheral blood mononuclear cells (PBMCs) in type 2 diabetes mellitus (T2DM) patients.Methods: Sixty patients, equally divided into those with T2DM and non-diabetic controls, were recruited for gene expression analysis. Protein-protein interaction (STRING), target prediction (miRNet), and functional enrichment were conducted accordingly.Results: Our study showed that VAT and PBMCs had similar expression profiles, where GDF-15 and miR-181b-5p were upregulated, whereas SMAD7 and miR-330-3p were downregulated. Serum GDF-15 could differentiate between T2DM and non-diabetic patients (P<0.001). Target prediction revealed a microRNA (miRNA)-messenger RNA (mRNA) regulatory network, transcription factors, and functional enrichment for the miRNA that suggested involvement in T2DM pathogenesis. Conclusion:VAT GDF-15 is associated with insulin resistance and is possibly regulated by miR-181b-5p, miR-330-3p, and SMAD7.

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Section: Discussionmentioning

confidence: 63%

Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus

Roy

Purohit

Khokhar

et al. 2023

Journal of Obesity & Metabolic Syndrome

View full text Add to dashboard Cite

show abstract

“…Blood mRNA studies on T2D patients without complications have identified elevated levels of inflammatory and senescence markers, as well as metabolically relevant receptor genes [ 61 , 62 , 63 , 64 ]. Plasma NF-kB expression levels were increased in pre-diabetic patients and further increased in T2D patients [ 62 ], suggesting that NF-kB can be a biomarker of early T2D diagnosis. In contrast, blood leptin mRNA levels were reduced in T2D patients compared to non-diabetic controls [ 65 ].…”

Section: Transcriptomics Biomarkers Of T2dmentioning

confidence: 99%

“…This miRNA is highly enriched in endothelial cells and is important for angiogenesis and wound repair [ 107 ]. It is down-regulated in T2D patients compared to non-diabetic controls and those with impaired glucose tolerance [ 62 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 ]. It is also further down-regulated in T2D patients with complications [ 111 , 115 , 119 ] and increases after six months of treatment (insulin with diet control and exercise) [ 116 ].…”

Section: Epigenomics Biomarkers For T2dmentioning

confidence: 99%

Circulating Nucleic Acid-Based Biomarkers of Type 2 Diabetes

Padilla-Martínez

Wojciechowska

Szczerbiński

et al. 2021

IJMS

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Type 2 diabetes (T2D) is a deficiency in how the body regulates glucose. Uncontrolled T2D will result in chronic high blood sugar levels, eventually resulting in T2D complications. These complications, such as kidney, eye, and nerve damage, are even harder to treat. Identifying individuals at high risk of developing T2D and its complications is essential for early prevention and treatment. Numerous studies have been done to identify biomarkers for T2D diagnosis and prognosis. This review focuses on recent T2D biomarker studies based on circulating nucleic acids using different omics technologies: genomics, transcriptomics, and epigenomics. Omics studies have profiled biomarker candidates from blood, urine, and other non-invasive samples. Despite methodological differences, several candidate biomarkers were reported for the risk and diagnosis of T2D, the prognosis of T2D complications, and pharmacodynamics of T2D treatments. Future studies should be done to validate the findings in larger samples and blood-based biomarkers in non-invasive samples to support the realization of precision medicine for T2D.

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Evaluation of miR-181b and miR-126-5p expression levels in T2DM patients compared to healthy individuals: Relationship with NF-κB gene expression

Cited by 2 publications

References 28 publications

Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus

Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus

Circulating Nucleic Acid-Based Biomarkers of Type 2 Diabetes

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