Evaluation of MiR-181a as a potential therapeutic target in osteoarthritis

Qian, Yuqiang; Jiang, Jiannong; Peng, Jun; Wang, Qiang; Shen, Yixin

doi:10.4314/tjpr.v16i5.14

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…The genetic locations of miR‐181a and miR‐181b are closely clustered together, as are miR‐181c and miR‐181d in humans, dogs, and mice. All members of the miR‐181 family have been shown to promote cell apoptosis by targeting proteins within the Bcl‐2 family that regulates the cell autophagy and apoptosis balance 42‐44 . In addition, miR‐181d targets leukemia inhibitory factor (LIF), which has been shown to enhance cardiac repair in rodent models after myocardial infarction 45,46 .…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicular microRNAs as potential biomarker for early detection of doxorubicin‐induced cardiotoxicity

Beaumier

Robinson

et al. 2020

Veterinary Internal Medicne

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Background: Long-term use of doxorubicin (DOX) is limited by cumulative dosedependent cardiotoxicity.Objectives: Identify plasma extracellular vesicle (EV)-associated microRNAs (miRNAs) as a biomarker for cardiotoxicity in dogs by correlating changes with cardiac troponin I (cTnI) concentrations and, echocardiographic and histologic findings.Animals: Prospective study of 9 client-owned dogs diagnosed with sarcoma and receiving DOX single-agent chemotherapy (total of 5 DOX treatments). Dogs with clinically relevant metastatic disease, preexisting heart disease, or breeds predisposed to cardiomyopathy were excluded.Methods: Serum concentration of cTnI was monitored before each treatment and 1 month after the treatment completion. Echocardiography was performed before treatments 1, 3, 5, and 1 month after completion. The EV-miRNA was isolated and sequenced before treatments 1 and 3, and 1 month after completion.Results: Linear mixed model analysis for repeated measurements was used to evaluate the effect of DOX. The miR-107 (P = .03) and miR-146a (P = .02) were significantly downregulated whereas miR-502 (P = .02) was upregulated. Changes in miR-502 were significant before administration of the third chemotherapeutic dose.When stratifying miRNA expression for change in left ventricular ejection fraction, upregulation of miR-181d was noted (P = .01). Serum concentration of cTnI changed significantly but only 1 month after treatment completion, and concentrations correlated with left ventricular ejection fraction and left ventricular internal dimension in diastole.Abbreviations: cTnI, cardiac troponin I; DOX, doxorubicin; E 0 , peak myocardial velocity in early diastole; ECG, electrocardiography; EV, extracellular vesicle; EV-miRNA, extracellular vesicle-associated miRNA; FAC, fractional area change; FS, fractional shortening; IVCT, isovolumic contraction time; IVRT, isovolumic relaxation time; LA:Ao, left atrium to aorta ratio; LVEF, left ventricular ejection fraction; LVIDdN, left ventricular end-diastolic internal diameter normalized to body weight; LVIDsN, left ventricular end-systolic internal diameter normalized to body weight; miRNAs, microRNAs; S 0 , peak myocardial velocity in systole.

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Section: Discussionmentioning

confidence: 99%

Extracellular vesicular microRNAs as potential biomarker for early detection of doxorubicin‐induced cardiotoxicity

Beaumier

Robinson

et al. 2020

Veterinary Internal Medicne

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“… 80 miR-181 played a stimulating role in apoptosis and decreased mitochondrial homeostasis of different cell types by targeting Bcl-2 and its associated receptor myeloid leukemia cell differentiation protein (Mcl-1). 81 , 82 , 83 Furthermore, Bcl-2 played a protective role for autophagy by binding to and stabilizing beclin1. The cardioprotective miR-30e, which is declined in rat cardiomyocytes after DOX, inhibited beclin 1, leading to a decrease in autophagy.…”

Section: Overview Of Potential Mechanisms Of Cardiotoxicity Associate...mentioning

confidence: 99%

Circulating MicroRNA as Biomarkers of Anthracycline-Induced Cardiotoxicity

Boen,

Cherubin,

Franssen

et al. 2024

JACC: CardioOncology

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“…The majority of the studies that performed RNA extraction from fresh cartilage samples, straightaway frozen the tissue in liquid nitrogen before performing the extraction procedure immediately after [8,[30][31][32][33][34][35][36][37][38][39][40][41][42][43], or stored them at −80 • C after freezing, until subsequent processing [1,7,[9][10][11]22,[44][45][46][47][48][49][50][51][52]. Alternatively, when immediate tissue freezing is not possible, the use of an RNA-stabilization solution should be considered.…”

Section: Tissue Collection and Storage Conditionmentioning

confidence: 99%

RNA Extraction from Cartilage: Issues, Methods, Tips

Pagani

Maglio

Sicuro

et al. 2023

IJMS

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The increase in degenerative diseases involving articular cartilage has pushed research to focus on their pathogenesis and treatment, exploiting increasingly complex techniques. Gene expression analyses from tissue are representative of the in vivo situation, but the protocols to be applied to obtain a reliable analysis are not completely cleared through customs. Thus, RNA extraction from fresh samples and specifically from musculoskeletal tissue such as cartilage is still a challenging issue. The aim of the review is to provide an overview of the techniques described in the literature for RNA extraction, highlighting limits and possibilities. The research retrieved 65 papers suitable for the purposes. The results highlighted the great difficulty in comparing the different studies, both for the sources of tissue used and for the techniques employed, as well as the details about protocols. Few papers compared different RNA extraction methods or homogenization techniques; the case study reported by authors about RNA extraction from sheep cartilage has not found an analog in the literature, confirming the existence of a relevant blank on studies about RNA extraction from cartilage tissue. However, the state of the art depicted can be used as a starting point to improve and expand studies on this topic.

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Evaluation of MiR-181a as a potential therapeutic target in osteoarthritis

Abstract: Purpose: To investigate

Cited by 7 publications

References 23 publications

Extracellular vesicular microRNAs as potential biomarker for early detection of doxorubicin‐induced cardiotoxicity

Extracellular vesicular microRNAs as potential biomarker for early detection of doxorubicin‐induced cardiotoxicity

Circulating MicroRNA as Biomarkers of Anthracycline-Induced Cardiotoxicity

RNA Extraction from Cartilage: Issues, Methods, Tips

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