Evaluation of MiR-15a and MiR-16-1 as prognostic biomarkers in chronic lymphocytic leukemia

Braga, Tatiane Vieira; Evangelista, Fernanda Cristina Gontijo; Gomes, Lorena Caixeta; Araújo, Sérgio Schusterschitz da Silva; Carvalho, Maria das Graças; Sabino, Adriano de Paula

doi:10.1016/j.biopha.2017.05.144

Cited by 34 publications

(28 citation statements)

References 39 publications

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“…≤ 0.05) were analyzed with DASHR and UCSC human genome browser for sequence annotations. Five of the novel miRs (novelmiR_4291, novelmiR_1520, novelmiR_1559, novelmiR_1732 and novelmiR_4370) showed homology with a multitude of tRNA molecules located on chromosomes 1,6,7,9,11,12,14,15,16, and 17 (supplementary table 1). The novelmiR_4370 showed homology with a piRNA-36225 (alias piRNA-28374; GenBank: DQ598159.1) as well.…”

Section: Annotation Of Novel Mirsmentioning

confidence: 99%

“…A few studies have identified karyotype specific miR signatures in CLL that could discriminate patients harboring del(17p), del(11q), del(13q), trisomy 12, and normal karyotype 12,13 . In patients with commonly encountered del(13q14), the co-localized tumor suppressive miR-15a and miR-16-1 get deleted, leading to increased BCL-2 expression that facilitates initiation of CLL 14 . Del(11q) is associated with co-deletion of miR-34b/ c clusters 15 as well as elevated levels of miR-769-5p and miR-338-3p 16 while trisomy 12 has been shown to be associated with up-regulation of miR-181a and down-regulation of miR-155, miR-148a, and miR-483-5p in CLL 12,16 .…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq profiling of deregulated miRs in CLL and their impact on clinical outcome

et al. 2020

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Abnormal expression patterns of regulatory small non-coding RNA (sncRNA) molecules such as microRNAs (miRs), piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs) play an important role in the development and progression of cancer. Identification of clinically relevant sncRNA signatures could, therefore, be of tremendous translational value. In the present study, genome-wide small RNA sequencing identified a unique pattern of differential regulation of eight miRs in Chronic Lymphocytic Leukemia (CLL). Among these, three were up-regulated (miR-1295a, miR-155, miR-4524a) and five were down-regulated (miR-30a, miR-423, miR-486*, let-7e, and miR-744) in CLL. Altered expression of all these eight differentially expressed miRs (DEMs) was validated by RQ-PCR. Besides, seven novel sequences identified to have elevated expression levels in CLL turned out to be transfer RNA (tRNA)/piRNAs (piRNA-30799, piRNA-36225)/snoRNA (SNORD43) related. Multivariate analysis showed that miR-4524a (HR: 1.916, 95% CI: 1.080-3.4, p value: 0.026) and miR-744 (HR: 0.415, 95% CI: 0.224-0.769, p value: 0.005) were significantly associated with risk and time to first treatment. Further investigations could help establish the scope of integration of these DEM markers into risk stratification designs and prognostication approaches for CLL.

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Section: Annotation Of Novel Mirsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNA-Seq profiling of deregulated miRs in CLL and their impact on clinical outcome

et al. 2020

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“…Since miR-16b and miR-100-3p are closely related to cell proliferation and apoptosis [33][34][35], the roles of miR-100-3p and miR-16b during cold acclimation were investigated. ZF4 cells were transfected with miR-100-3p mimics, miR-16b mimics, miR-100-3p inhibitor or miR-16b inhibitor, respectively, and incubated at 10˚C as cold treatment, then cell viability was detected.…”

Section: Roles Of Mir-100-3p and Mir-16b During Cold Acclimationmentioning

confidence: 99%

Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells

Jiang

Luo

et al. 2020

PLoS ONE

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MicroRNAs (miRNAs) play vital roles in various biological processes under multiple stress conditions by leading to mRNA cleavage or translational repression. However, the detailed roles of miRNAs in cold acclimation in fish are still unclear. In the present study, highthroughput sequencing was performed to identify miRNAs from 6 small RNA libraries from the zebrafish embryonic fibroblast ZF4 cells under control (28˚C, 30 days) and cold-acclimation (18˚C, 30 days) conditions. A total of 414 miRNAs, 349 known and 65 novel, were identified. Among those miRNAs, 24 (19 known and 5 novel) were up-regulated, and 23 (9 known and 14 novel) were down-regulated in cold acclimated cells. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses indicated that the target genes of known differentially expressed miRNAs (DE-miRNA) are involved in cold acclimation by regulation of phosphorylation, cell junction, intracellular signal transduction, ECM-receptor interaction and so on. Moreover, both miR-100-3p inhibitor and miR-16b mimics could protect ZF4 cells under cold stress, indicating the involvement of miRNA in cold acclimation. Further study showed that miR-100-3p and miR-16b could regulate inversely the expression of their target gene (atad5a, cyp2ae1, lamp1, rilp, atxn7, tnika, btbd9), and that overexpression of miR-100-3p disturbed the early embryonic development of zebrafish. In summary, the present data show that miRNAs are closely involved in cold acclimation in zebrafish ZF4 cells and provide information for further understanding of the roles of miRNAs in cold acclimation in fish.

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“…A certain subset of CRC arises from the sequential accumulation of genetic and epigenetic alterations. microRNAs (miRNA/miR) have been revealed as candidates in tumor progression in various cancer types, and changes in their expression levels are consequently being investigated with the purpose of identifying clinical biomarkers (3)(4)(5)(6)(7)(8)(9). miRNAs constitute a class of small, non-coding RNA molecules, [18][19][20][21][22][23][24][25][26][27] nucleotides in length that function as post-transcriptional regulators of gene expression, either serving as oncogenes or tumor suppressor genes (10,11).…”

Section: Introductionmentioning

confidence: 99%

Upregulation of microRNA‑31 is associated with poor prognosis in patients with advanced colorectal cancer

et al. 2020

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Colorectal cancer (CRC) manifests after the accumulation of genetic and epigenetic alterations along with tumor microenvironments. MicroRNA (miRNA/miR) molecules have been revealed to serve in critical roles in the progression various types of cancer, and their expression level is often an important diagnostic, predictive or prognostic biomarker. The aim of the present study was to evaluate the potential of miRNAs as prognostic biomarkers for patients with advanced CRC. miRNA arrays were performed on CRC specimens obtained from tumors with various molecular statuses [e.g. KRAS proto-oncogene, GTPase (KRAS)/B-Raf proto-oncogene, serine/threonine kinase (BRAF)/microsatellite instability (MSI)], and their paired normal mucosal specimens. The miRNA array revealed that miR-31-5p (miR-31) was specifically upregulated in CRCs with the BRAF V600E mutation, the results of which were supported by subsequent analysis of a dataset retrieved from The Cancer Genome Atlas (TCGA) database, which contained information regarding 170 patients with CRC including 51 BRAF-mutant CRCs. Of our cohort of 67 patients with stage IV CRC, 15 (22%) and 4 (6%) showed KRAS and BRAF V600E mutations, respectively. Since the median miR-31 expression was 3.45 (range, 0.004-6330.531), the cut-off value was chosen as 3.5, and all tumors were categorized into two groups accordingly (high-/low-miR-31 expression). The high miR-31 expression group (n=33) was significantly associated with a poorer mortality (univariate hazard ratio=2.12; 95% confidence interval, 0.23-0.95; P= 0.03) and exhibited a shorter median survival time (MST; 20.1 months) compared with the low miR-31 expression group (n=34) (MST, 38.3 months; P=0.03), indicating that miR-31 is a promising prognostic biomarker for patients with advanced CRC. Thus, performing a functional analysis of miR-31 expression may lead to the development of new targeted therapies for the various genetic subtypes of CRC.

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Evaluation of MiR-15a and MiR-16-1 as prognostic biomarkers in chronic lymphocytic leukemia

Cited by 34 publications

References 39 publications

RNA-Seq profiling of deregulated miRs in CLL and their impact on clinical outcome

RNA-Seq profiling of deregulated miRs in CLL and their impact on clinical outcome

Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells

Upregulation of microRNA‑31 is associated with poor prognosis in patients with advanced colorectal cancer

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