Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

Silva, P.R.O.; Jesus, O. N. de; Creste, Silvana; Figueira, Antônio; Amorim, Édson Perito; Ferreira, Cláudia Fortes

doi:10.4238/2015.september.25.9

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…In this sense, different works have been carried out for characterization of SSR markers from M. balbisiana (Buhariwalla et al, 2005;Ravishankar et al, 2013;Wang et al, 2011) and M. acuminata (Amorim et al, 2012;Creste et al, 2006;Crouch et al, 1997Crouch et al, , 1998Miller et al, 2010;Wang et al, 2010), whereas other studies have been focused on the development of SSR markers suitable for analysis of both species and/or hybrid cultivars (Brisibe & Ubi, 2020;Kaemmer et al, 1997;Lagoda et al, 1998;Ravishankar et al, 2012;Silva et al, 2015). In other publications, sets of primers previously developed have been used for molecular characterization of Musa varieties (Bawin et al, 2019;Durai et al, 2018;Grapin et al, 1998), including cultivars from certain regions as Brazil (Creste et al, 2003(Creste et al, , 2004, Malawi (Changadeya et al, 2012), East Africa (Nzawele et al, 2018;Onyango et al, 2008;Perrier et al, 2019), Indonesia (Retnoningsih et al, 2009), the Philippines (dela Cruz et al, 2020) or Tanzania (Mgonja et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

et al. 2021

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Banana cultivars of agronomic interest have been genetically characterized using two different molecular markers. On the one hand, a panel of 14 trinucleotide single sequence repeats (SSRs or microsatellites) was optimized for homogeneous PCR conditions. It was tested with 50 individuals from seven cultivars, yielding 76 alleles and 5.4 ± 1.8 alleles per locus, while the presence of cultivar-exclusive alleles allowed the discrimination of all cultivars. On the other hand, a retrotransposon-based marker system named inter-primer binding site (iPBS) was implemented for the first time in the Musa genus. A total of 120 bands were detected in eight different Musa cultivars, from which 65.8% were polymorphic and 23.3% were cultivar exclusive. Both techniques allowed a cut-off identification of all cultivars studied, but overall, iPBS analysis was a more straightforward and economical choice. Despite the fact that we were unable to distinguish local banana varieties belonging to the same cultivar, new cultivar-specific molecular markers have been developed for the Musa genus, which could be used to guide new breeding programmes and maintain high quality of Plátano de Canarias.

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Section: Introductionmentioning

confidence: 99%

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

et al. 2021

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“…In recent research, many studies have been carried out on microsatellites on Musa spp. (Biswas et al, 2015;Christelova et al, 2016;Silva et al, 2015) providing information for further research.…”

Section: Introductionmentioning

confidence: 99%

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Quain¹,

Agyeman²,

Okyere³

et al. 2018

Int. J. Genet. Mol. Biol.

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Plantains are important staples in sub-Saharan Africa, however, production has been saddled by several constraints including pests and diseases and lack of improved technologies. In regard to genetic study, morphological characterization has identified about 100 local accessions to exist in Ghana. However, limited molecular characterization has been done on plantains. Characterization based on morphologic characteristics alone may be limited since expression of quantitative traits is subjective to strong environmental influence. Alternatively, molecular characterization techniques are capable of identifying polymorphism represented by differences in DNA sequences. The objective of this research was therefore to characterize local accessions of plantain using morphological and molecular techniques. This study sampled 38 local accessions from two regions in Ghana for characterization, taking into account all known morphological characteristics. Genomic DNA was isolated for molecular characterization using 18 SSR markers and 10 random primers. The SSR markers revealed 52 alleles, random primers revealed 67 alleles and the 29 morphological traits (loci) had 79 attributes (alleles). Similarity matrices of SSR markers revealed that 26.5% of the plantain accessions were duplicates; however, random amplified polymorphic DNA (RAPDs) system and morphological marker systems did not reveal any duplication among genotypes. Dendrogram generated on bases of both SSR and RAPDs indicated that a French plantain accession (Apem 01) and a False Horn accession (Apantu 26) were the most distantly related collections. None of the molecular marker methods used clearly clustered various phenotypically known collections into their respective distinct groups, although to some extent, this was demonstrated by morphological data set.

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“…An insight of genetic make-up may contribute to information, vital for both breeders and consumers. Several methods have been used to investigate the genetic variability present in Musa germplasm (Silva et al, 2015;Hippolyte et al, 2010). The development and application of technologies based upon molecular markers provide the only tools that are able to reveal polymorphism at the DNA sequence level, which are adequate to detect genetic variability between individuals and within populations (Kresovich et al, 1995) which will facilitate breeding efforts to improve the crop against biotic and abiotic stresses (Rodrigues et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Assessment of plantain (Musa sapientum L.) accessions genotypic groups relatedness using simple sequence repeats markers

Quain¹,

Agyeman²,

Dzomeku³

2018

Afr. J. Biotechnol.

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Plantains are important sources of high-calorie energy in Ghana. They are also of great socio-economic importance in the country, and very important sources of rural income. Although several species exist all over the world, plantains belonging to the AAB group are unique to West Africa and Ghanaian collections have unique features and peculiar taste. Morphological and biochemical characterization are the popular techniques used to characterize plantain genotypes in Ghana. Thus, there is limited report on molecular characterization of plantains genotypes. Characterization based on morphologic characteristics alone may be limited since the expression of quantitative traits is subjective to strong environmental influence. Alternatively, molecular characterization techniques are capable of identifying polymorphism represented by differences in DNA sequences. The objective of this research was therefore to conduct molecular characterization of Ghanaian local accessions of plantain and assess relationship amongst known genotypic groups (populations). This study sampled 40 accessions of plantains representing four popular genotypic groups. Simple Sequence Repeats (SSRs) were used to assess diversity in reference to a set of global Musa collections. The 40 accessions of plantain were clustered into populations as being French plantain, True Horn, False Horn, and Hybrid prior to analysis. PopGene version 32 was used to analyze the data. This revealed that the overall plantain population used have Shannon's Informative Index (I) value of 0.61±0.28 in the overall plantain population, 100% polymorphism for all loci, 2.7±0.67 and 1.81±0.45 for ne and na respectively. Average heterozygosity was 0.34±0.17, loci mMaCIR231 and mMaCIR07 were the most informative, having I values of 0.85 and 0.81 respectively. The Fis and Fit values were both negative indicating lack of inbreeding and the gene flow value was 0.533. The study also revealed relationship among the various populations (French plantain, True Horn, False Horn, and Hybrid) on basis of molecular characterization.

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Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

Cited by 4 publications

References 26 publications

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Assessment of plantain (Musa sapientum L.) accessions genotypic groups relatedness using simple sequence repeats markers

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