Evaluation of microbial antagonists and essential oils in the control of Sclerotium cepivorum in garlic under controlled conditions

Zapata-Narváez, Yimmy Alexander; Gómez-Marroquín, Magda; Botina-Azain, Blanca Lucia

doi:10.18781/r.mex.fit.2002-2

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…The microbial biomass of liquid biopesticides has a larger inhibition zone than the biochemical content. Other studies have also proven the effect of antagonistic microbes in inhibiting the growth of the phytopathogen with inhibition up to 95%, higher than the inhibition of oregano and eucalyptus essential oils, which is 92% [41]. This is related to the mode of action of antagonistic microbes, which is specific or has a narrow range of pest species [42].…”

Section: Figure 3 Importance Value Index (Ivi) Of Nine Bacterial Isol...mentioning

confidence: 97%

Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot

Aini,

Suharjono

2024

Jurnal Biotropika

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Shallot base rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses, thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that 2 bacterial isolates B7 and B2 had the highest Importance Value Index of 94.21% and 90.59% respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.

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Section: Figure 3 Importance Value Index (Ivi) Of Nine Bacterial Isol...mentioning

confidence: 97%

Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot

Aini,

Suharjono

2024

Jurnal Biotropika

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“…This saprophytic aerobic fungus produces a great variety of metabolites, including enzymes such as cellulases, glucanases and chitinases (Cotes 2011). Due to the production of these enzymes, T. koningiopsis Th003 can degrade sclerotia from phytopathogenic fungi such as R. solani, Sclerotinia sclerotiorum and Sclerotium cepivorum (Smith et al 2013;Zapata et al 2020). Th003 is the active ingredient of the waterdispersible granules formulated bio-fungicide TRICOTEC® WG.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel capsules as new delivery system for Trichoderma koningiopsis Th003 to control Rhizoctonia solani in rice (Oryza sativa)

Barrera

García

Gómez-Marroquín

et al. 2022

Preprint

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Biological control (BCAs) such as Trichoderma spp. minimize fungicides inputs in agriculture, and increase both the plant nutrition and the efficacy against fungal diseases. Novel bioproducts in crop management are called to guarantee sustainable antagonism activity of BCAs and increase the acceptance of the farmers. The encapsulation in polymeric matrices play a prominent role for providing an effective carrier/protector and long-lasting bioproduct. This research aimed to compare two hydrogel capsules prototypes based on alginate (P1) and amidated pectin (P2), containing Trichoderma koningiopsis Th003 conidia. Capsules were prepared by the ionic gelation method and calcium gluconate as crosslinker was used. The capsules were characterized by various properties such as conidia releasing at different pH values, drying conidia survival, storage stability, and biocontrol activity against sheath blight (R. solani) in rice. P2 prototype provided higher survival to Th003 up to 98% in fluid bed drying, faster conidia release at pH 5.8, storage stability > 6 months at 18°C, and a disease reduction up to 67%. Amidated pectin provides advantages on drying survival and conidia release at pH 5.8. Both biopolymers facilitate the antagonistic activity against R. solani, and therefore can be incorporated in hydrogel capsules for novel bioinoculants development. This research highlights the knowledge regarding the influence of biopolymer in hydrogel capsules on survival and shelf-life of Trichoderma spp conidia.

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Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

Moreno-Velandia,

Izquierdo-García,

García-Arias

et al. 2024

Eur J Plant Pathol

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Clubroot disease caused by Plasmodiophora brassicae is the most devastating disease in Brassicaceae plants. Control of clubroot is limited because of the survival of resting spores of the pathogen in the soil for years. Crop rotation, liming of the soil, fungicides and resistant cultivars have not been totally efficient in reducing the damages of the disease or pathogen spread. Although biocontrol with microorganisms has shown potential against clubroot, the variable results when using individual strains have made implementation difficult. Thus, the aim of this work was to determine whether a consortium of selected beneficial microorganisms is more efficient in controlling clubroot in broccoli than single strains. Single strains of bacteria from the Lysinibacillus, Bacillus and Pseudomonas genera (5 × 107 cells ml−1) and Trichoderma spp. (1 × 106 conidia ml−1) were evaluated in an initial screening. Four strains showing the best performance on plant growth and reduction of clubroot were evaluated as consortia in a further experiment. Treatments were applied in nursery (4 ml plant−1), and to the soil one week before transplant and the day of transplant (60 ml plant−1 each time). Plant shoot and root dry weight and the incidence and severity of clubroot were recorded. Supernatant from liquid cultures of Pseudomonas migulae Pf014 and Lysinibacillus xylanilyticus Br042, and washed cells of Bacillus velezensis Bs006 and B. pumilus Br019 promoted shoot growth of the plants. Single applications of Trichoderma asperellum Th034, Bs006, Br042 and Pseudomonas fluorescens Ps006 showed biocontrol potential against clubroot. However, the consortia built of Bs006, Br042 and Ps006 showed the highest reduction of clubroot incidence (78%) and severity (74%). A synergistic interaction by this consortium was found compared with the efficacy of single strains. To our knowledge, this work reports for the first time a consortium based on three rhizobacteria with high potential to control clubroot disease.

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Evaluation of microbial antagonists and essential oils in the control of Sclerotium cepivorum in garlic under controlled conditions

Cited by 4 publications

References 23 publications

Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot

Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot

Hydrogel capsules as new delivery system for Trichoderma koningiopsis Th003 to control Rhizoctonia solani in rice (Oryza sativa)

Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

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