Evaluation of micro-fungi associated with leaf spot of Allanblackia floribunda Oliv. in Southern Nigeria

Olasupo, Olusola O.; Adegeye, Adebola O.; Olajuyigbe, Samuel

doi:10.5897/ajmr2020.9307

Cited by 14 publications

(2 citation statements)

References 17 publications

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“…After solidification, surface-sterilized samples were inoculated with the help of clean forceps. The inoculated samples were incubated at 28 °C for 15 days [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

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Bioactive Components and Health Potential of Endophytic Micro-Fungal Diversity in Medicinal Plants

Muthukrishnan¹,

Prakathi²,

Sivakumar³

et al. 2022

Antibiotics

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The endophytic fungi that reside inside medicinal plants have the potential to produce various pharmaco-potential bioactive compounds. The endophytic fungi Graminicolous helminthosporium, Bipolaris australiensis and Cladosporium cladosporioides were isolated from different medicinal plants. The GC-MS analysis of intra- and extracellular products of endophytic fungi revealed the presence of various bioactive metabolites, such as Anthracene, Brallobarbital, Benzo [h] quinolone, Ethylacridine, 2-Ethylacridine, Cyclotrisiloxane, 5 methyl 2 phenylindolizine, and 1,4-Cyclohexadien-1-one, etc. The phytochemical composition analysis of endophytic fungus extracts also revealed the presence of flavonoids, phenols, saponins, carbohydrates, glycosides, and proteins. The intra- and extracellular endophytic extracts exhibited strong antibacterial and antioxidant activity, which was screened with the agar-well diffusion method and DPPH, H2O2, and nitric oxide scavenging activity, respectively. The bioactive compounds identified in the endophytic extracts from GC-MS profiling served as ligands for molecular-docking analysis to investigate the anticancer potential against non-small cell lung carcinoma receptor EGFR. Molecular docking results showed that compounds, such as Brallobarbital, and 5 methyl 2 phenylindolizine had the lowest E- min values, which suggests that these compounds could be used in anticancer drug development. Thus, the isolated endophytic fungal species can be used to produce various bioactive compounds that could be used in novel drug development from natural sources and reduce the environmental burden of synthetic chemical drugs.

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“…After solidification, surface-sterilized samples were inoculated with the help of clean forceps. The inoculated samples were incubated at 28 °C for 15 days [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…The fungal strain was taken with a sterile needle and placed on the microscopic slides loaded with a few drops of stain. Then the fungal strain in the slides was subjected to teasing to spread the conidia and spores of the fungi and then examined under a microscope [ 11 ].…”

Section: Methodsmentioning

confidence: 99%