Evaluation of methods for pore generation and their influence on physio-chemical properties of a protein based hydrogel

Abstract

HighlightsGeneration of a THPC linked BSA hydrogel and characterization by cryo electron microscopy.Evaluation of techniques to create tunable pore sizes and shapes including channel like structures.Characterization of materials by confocal laser scanning microscopy.Modification of hydrogels with cell adhesive peptides (RGD).Comparison of cancer cell adhesion (A549 and MCF7) on the material.

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

“…These engineered scaffolds promoted the hepatocyte cell function, characterized by large colony formation in the predefined chambers within 1 week, which secreted albumin and urea more effectively than highly porous materials . These scaffolds were prepared through the combination of solid freeform fabrication (SFF), microreplication, and freeze‐drying approaches . The fabrication process is described in Figure a.…”

“…[161][162][163] These scaffolds were prepared through the combination of solid freeform fabrication (SFF), 164 microreplication, 165 and freeze-drying approaches. 166 The fabrication process is described in Figure 4a. Initially, the desired shape was programmed using a computer-aided design (CAD) software from which a resin mold, typically from polydimethylsiloxane (PDMS), was prepared.…”

A Cerberus‐Inspired Anti‐Infective Multicomponent Gatekeeper Hydrogel against Infections with the Emerging “Superbug” Yeast Candida auris

“…Since the overall hardness of the composite material mainly depends on the elastic properties of the carrier layer (Figure 2F) and thus on the BSA concentration. [ 29,31 ] This promises that the mechanical properties of the intended final wound dressing can be tuned, without disturbing the burst release of an encapsulated drug, necessary for a convenient fast active treatment. The release rate of the 10% BSA hydrogel as a prototype reached its maximum within the first 2 h (Figure 4G), resulting in an almost complete release of the compound.…”

“…[ 15 ] The purified YFP‐LecB fusion protein was fully functional in binding C. auris and thus labeling the cells for fluorescence microscopy ( Figure 5 A). The BSA/THPC hydrogel, whose properties can easily be tailored and which has been shown to be biocompatible, [ 29,31 ] was decorated with recombinant YFP‐LecB by chemical cross‐linking [ 15 ] using bismaleimideoethane with fluorescence signals of the labeled material linear increasing with YFP‐LecB concentrations and resulting in homogenous functionalization of the material (Figure 5B). The increasing YFP‐LecB coverage was almost identical with an increase of binding capacity of the YFP‐LecB hydrogel for C. auris reaching a complete coverage of approximately 90 000 cells mm −2 at lectin concentrations of 300 × 10 −9 m, which is in accordance with the calculated value for the maximal coverage of 90 000 cells mm 2 taking an average yeast diameter of 3–4 µm into account [ 4 ] (Figure 5C,D).…”

Image analysis method for heterogeneity and porosity characterization of biomimetic hydrogels

“…To validate the method, the screenshot images of a hydrogel network from a previously published work where the corresponding pore diameters have been reported, were analysed with the proposed method using OTSU threshold and setting 0.05 max watershed threshold. The obtained results of the proposed method (17 µm) were compared with the available reported measurement (15 µm from Figure 4) 10 .…”