Evaluation of methods for nitrogen‐15 analysis of inorganic nitrogen in soil extracts. II. Diffusion methods

Herman, Donald J.; Brooks, P. D.; Ashraf, Muhammad; Azam, F.; Mulvaney, R. L.

doi:10.1080/00103629509369400

Cited by 47 publications

(29 citation statements)

References 7 publications

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“…We assumed that the background 15 N enrichment of the NH 4 þ and NO 3 À pools were at natural abundance, 0.3663 atom %. The 15 N enrichment of NH 4 þ and NO 3 À were determined using the diffusion technique (Herman et al, 1995) and analysis on an isotope ratio mass spectrometer (PDZ Europa, Limited, Crewe, UK). Gross N mineralization and nitrification rates as well as gross NH 4 þ and NO 3 À consumption rates were calculated according to Kirkham and Some study sites were co-located, leading to 27 distinct locations represented; global positioning system (GPS) coordinates for each site are listed in Table S1.…”

Section: Gross Nitrogen Cycling Assaysmentioning

confidence: 99%

Cross-biome assessment of gross soil nitrogen cycling in California ecosystems

Yang

Ryals

Cusack

et al. 2017

Soil Biology and Biochemistry

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Section: Gross Nitrogen Cycling Assaysmentioning

confidence: 99%

Cross-biome assessment of gross soil nitrogen cycling in California ecosystems

Yang

Ryals

Cusack

et al. 2017

Soil Biology and Biochemistry

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“…Soil extracts were analyzed colorimetrically for NH 4 þ and NO 3 À concentrations on a Lachat autoanalyzer (Milwaukee, WI). Soil extracts were prepared for 15 N isotope analysis by acid-trap diffusion (Herman et al 1995) and analyzed using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (IRMS; Sercon Ltd., Cheshire, UK) at the University of California, Davis (UCD).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Microbially mediated nitrogen retention and loss in a salt marsh soil

2015

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Abstract. Salt marshes currently play an important role as filters for upslope nitrogen (N) inputs. This could change in the future with sea level rise, warming and eutrophication, which are expected to favor monocultures over diverse plant communities. We explored patterns in gross N cycling, dissimilatory nitrate (NO 3 À ) reduction to ammonium (NH 4 þ ) (DNRA), and denitrification in a salt marsh soil under two typical redox conditions (aerobic and anaerobic), and in soils under plant communities manipulated to simulate potential future composition (forb and graminoid monocultures). Natural salt marsh soils exhibited high potential gross N mineralization rates, averaging 50.4 6 5.7 lg N g À1 d À1 under aerobic conditions; rates declined to 23.6 6 3.4 lg N g À1 d À1 under an N 2 headspace. Microbial NH 4 þ uptake and gross nitrification together accounted for only 14 % of gross N mineralization. Nitrogen retention via DNRA and microbial uptake greatly exceeded N losses via denitrification. Gross nitrification rates were greater in the forb and graminoid monocultures than in the control. This effect may be mediated by the lower plant biomass in the monocultures than in the control, which may have reduced competition between plants and nitrifiers for NH 4 þ . Soil NO 3 À concentrations and net nitrous oxide (N 2 O) fluxes were greatest for the forb monoculture, likely due to higher soil oxygen (O 2 ) concentrations in these plots. Our results suggest that salt marsh soils with a diverse plant community have high potential rates of N mineralization and microbial N retention, and the establishment of forb monocultures could lead to greater ecosystem N losses.

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“…We determined NH 4 + and NO 3 -concentrations colorimetrically (Lachat QuikChem 8000 Flow Injector Analyzer, Latchat-Zellwger Instruments, Milwaukee WI USA). Extracts were prepared for isotope analysis by diffusion (Herman et al 1995), and N-isotope ratios were measured using an automated nitrogen-carbon analyzer coupled to an isotope ratio mass spectrometer (ANCA-IRMS; PDZ Europa Limited, Crew, UK). Gravimetric soil moisture was determined for all soil samples by drying 10 g soil at 105 o C to a constant weight.…”

Section: Initial Processing Of Samples Was Done In the Field And At Tmentioning

confidence: 99%

Plant and Microbial Controls on Nitrogen Retention and Loss in a Humid Tropical Forest

Templer

Silver

Pett‐Ridge

et al. 2008

Ecology

148

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Humid tropical forests are generally characterized by the lack of nitrogen (N) limitation to net primary productivity, yet paradoxically have high potential for N loss. were not measured in our fluxes, suggesting that other important pathways for N retention and loss (e.g., denitrification to N 2 ) are important in this system. The high proportion of mineralized N that was rapidly nitrified and the fates of that NO 3 -highlight the key role of gross nitrification as a proximate control on N retention and loss in humid tropical forest soils. Furthermore, our results demonstrate the importance of the coupling between DNRA and plant uptake of NH 4 + as a potential N conserving mechanism within tropical forests.3

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Evaluation of methods for nitrogen‐15 analysis of inorganic nitrogen in soil extracts. II. Diffusion methods

Cited by 47 publications

References 7 publications

Cross-biome assessment of gross soil nitrogen cycling in California ecosystems

Cross-biome assessment of gross soil nitrogen cycling in California ecosystems

Microbially mediated nitrogen retention and loss in a salt marsh soil

Plant and Microbial Controls on Nitrogen Retention and Loss in a Humid Tropical Forest

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